2026-03-09T08:47:06Z https://tokushima-u.repo.nii.ac.jp/oai

oai:tokushima-u.repo.nii.ac.jp:02001579 2025-04-28T06:01:25Z 1713829974766:1713854681789:1716364517058:1716365669729 1713853213384:1713853295607

Gene gun-mediated skin transfection with FL gene suppresses the growth of murine fibrosarcoma 30748588 阿部, 彰子 アベ, アキコ Abe, Akiko 阿部 アベ Abe 彰子 アキコ Akiko 古本, 博孝 フルモト, ヒロユキ Furumoto, Hiroyuki 吉田, 加奈子 ヨシダ, カナコ Yoshida, Kanako 吉田 ヨシダ Yoshida 加奈子 カナコ Kanako 加藤, 剛志 カトウ, タケシ Kato, Takeshi 西條, 康代 サイジョウ, ヤスヨ Saijo, Yasuyo 西條 サイジョウ Saijo 康代 ヤスヨ Yasuyo 20160070 苛原, 稔 イラハラ, ミノル Irahara, Minoru 苛原 イラハラ Irahara 稔 ミノル Minoru open access fms-like tyrosine kinase 3 ligand gene gun dendritic cells Aim : Particle-mediated transfection is known as an efficient method of nonviral gene transfer. Flt3 ligand (FL) is a growth factor for hematopoietic progenitors ; it promotes the growth of dendritic cells (DC). DCs are powerful antigen-presenting cells (APCs) and show a remarkable capacity to stimulate antigen-specific T-cell responses. In this study, we intended to investigate the suppressive effect on tumor growth by gene gun-mediated transfer of FL in a murine model. Methods : C57BL/6J mice were injected intradermally with MCA205 cells. DNA (pNGVL-hFLex)-coated gold particles were delivered to themouse skin surrounding the target tumor. The expression of FL was determined by RT-PCR. Analyses by immunohistochemistry and fluorescence-activated cell sorter (FACS) revealed an increase in the number of DC after treatment with FL. Results : Gene gun-mediated pNGVL-hFLex transfer significantly inhibited the growth of the MCA205 tumor. FL transfer markedly increased the number of CD11c+ DCs in the tumor tissue. Further, the FL-transfected mice exhibited a significantly higher number of CD80+ MHC-II cells. Conclusion : We successfully performed FL therapy using an in vivo gene gun in order to effectively mobilize DCs in situ and induce suppressive immunity. 2011-02 eng journal article VoR 248981 https://tokushima-u.repo.nii.ac.jp/records/2001579 13431420 AA11166929 The journal of medical investigation : JMI 58 1-2 39 45 https://tokushima-u.repo.nii.ac.jp/record/2001579/files/LID201112081005.pdf application/pdf 255 KB 2011-12-08