2026-03-15T20:47:33Z https://tokushima-u.repo.nii.ac.jp/oai

oai:tokushima-u.repo.nii.ac.jp:02003997 2025-04-28T06:01:33Z 1713829974766:1713854681789:1716364518663:1716421310005 1713853213384:1713853295607

A rapid and simple system of detecting deletions on the Y chromosome related with male infertility using multiplex PCR 梅野, 真由美 ウメノ, マユミ Umeno, Mayumi 新家, 利一 シンカ, トシカツ Shinka, Toshikatsu 10363160 佐藤, 陽一 サトウ, ヨウイチ Sato, Youichi 佐藤 サトウ Sato 陽一 ヨウイチ Youichi Yang, Xin-Jun 馬場, 嘉信 ババ, ヨシノブ Baba, Yoshinobu イワモト, テルアキ イワモト, テルアキ Iwamoto, Teruaki 中堀, 豊 ナカホリ, ユタカ Nakahori, Yutaka open access Y chromosome AZF multiplex PCR microchip electrophoresis azoospermia Around 10% of males with idiopathic azoospermia or oligozoospermia, which are important causes of male infertility, have partial deletions on the long arm of the Y chromosome. To develop a rapid and accurate detection system for screening major Y deletions found in infertile men, we developed a multiplex PCR system that can simultaneously amplify five loci on the Y chromosome, SRY, AMELY, DBY, RBMY, DAZ and one locus on the X chromosome, AMELX. The size of the PCR products was designed to increase gradually from the distal Yp to the distal Yq. Our system could detect deletions of three major candidate regions for the azoospermic factor, AZFa, AZFb and AZFc on the Y chromosome together with sex. The gradual increase in the size of the PCR products was convenient for imaging the location of deletions on the Y chromosome. Moreover, the multiplex PCR system was combined with microchip-based electrophoresis, and the PCR products derived from each locus were separated within 4min. Our system is useful for screening Y chromosomes bearing the structural anomalies including three major AZF deletions found among azoospermic or oligozoospermic males. 2006-02 eng journal article VoR 160577 https://tokushima-u.repo.nii.ac.jp/records/2003997 13431420 AA11166929 The journal of medical investigation : JMI 53 1-2 147 152 https://tokushima-u.repo.nii.ac.jp/record/2003997/files/jmi_53_1-2_147.pdf application/pdf 122 KB 2017-12-07