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oai:tokushima-u.repo.nii.ac.jp:02004087 2025-04-28T06:42:49Z 1713853213384:1713853295607

Structure models of G72, the product of a susceptibility gene to schizophrenia 70596816 加藤, 有介 カトウ, ユウスケ Kato, Yusuke 加藤 カトウ Kato 有介 ユウスケ Yusuke 00175564 福井, 清 フクイ, キヨシ Fukui, Kiyoshi 福井 フクイ Fukui 清 キヨシ Kiyoshi open access G72 ab initio method structure prediction schizophrenia D-amino acid oxidase The G72 gene is one of the most susceptible genes to schizophrenia and is contained exclusively in the genomes of primates. The product of the G72 gene modulates the activity of D-amino acid oxidase (DAO) and is a small protein prone to aggregate, which hampers its structural studies. In addition, lack of a known structure of a homologue makes it difficult to use the homology modeling method for the prediction of the structure. Thus, we first developed a hybrid ab initio approach for small proteins prior to the prediction of the structure of G72. The approach uses three known ab initio algorithms. To evaluate the hybrid approach, we tested our prediction of the structure of the amino acid sequences whose structures were already solved and compared the predicted structures with the experimentally solved structures. Based on these comparisons, the average accuracy of our approach was calculated to be ∼5 Å. We then applied the approach to the sequence of G72 and successfully predicted the structures of the N- and C-terminal domains (ND and CD, respectively) of G72. The predicted structures of ND and CD were similar to membrane-bound proteins and adaptor proteins, respectively. Oxford University Press 2016-11-03 eng journal article AM 322896 https://tokushima-u.repo.nii.ac.jp/records/2004087 https://doi.org/10.1093/jb/mvw064 17562651 0021924X AA12096002 AA00694073 The Journal of Biochemistry 161 2 223 230 https://tokushima-u.repo.nii.ac.jp/record/2004087/files/jb_161_2_223.pdf application/pdf 7.64 MB 2017-11-22