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Bone morphogenetic protein signaling in distal patterning and intercalation during leg regeneration of the cricket, Gryllus bimaculatus BMP signaling in distal patterning and intercalation during leg regeneration of the cricket, Gryllus bimaculatus Role of BMP signaling in leg regeneration 50435525 石丸, 善康 イシマル, ヨシヤス Ishimaru, Yoshiyasu 石丸 イシマル Ishimaru 善康 ヨシヤス Yoshiyasu バンドウ, テツヤ バンドウ, テツヤ Bando, Tetsuya 00253229 大内, 淑代 オオウチ, ヒデヨ Ohuchi, Hideyo 大内 オオウチ Ohuchi 淑代 ヒデヨ Hideyo 40156211 野地, 澄晴 ノジ, スミハレ Noji, Sumihare 野地 ノジ Noji 澄晴 スミハレ Sumihare 80322254 三戸, 太郎 ミト, タロウ Mito, Taro 三戸 ミト Mito 太郎 タロウ Taro open access This is the peer reviewed version of the following article: Ishimaru, Y, Bando, T, Ohuchi, H, Noji, S, Mito, T. Bone morphogenetic protein signaling in distal patterning and intercalation during leg regeneration of the cricket, Gryllus bimaculatus. Develop. Growth Differ. 2018; 60: 377– 386., which has been published in final form at https://doi.org/10.1111/dgd.12560. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited. Dachsous/Fat decapentaplegic Gryllus bimaculatus intercalation leg regeneration The cricket, Gryllus bimaculatus, is a classic model of leg regeneration following amputation. We previously demonstrated that Gryllus decapentaplegic (Gb’dpp) is expressed during leg regeneration, although it remains unclear whether it is essential for this process. In this study, double-stranded RNA targeting the Smad mathers-against-dpp homolog, Gb’mad, was employed to examine the role of Bone morphogenetic protein (BMP) signaling in the leg regeneration process of Gryllus bimaculatus. RNA interference (RNAi)-mediated knockdown of Gb’mad led to a loss of tarsus regeneration at the most distal region of regenerating leg segments. Moreover, we confirmed that the phenotype obtained by knockdown of Dpp type I receptor, Thick veins (Gb’tkv), closely resembled that observed for Gb’mad RNAi crickets, thereby suggesting that the BMP signaling pathway is indispensable for the initial stages of tarsus formation. Interestingly, knockdown of Gb’mad and Gb’tkv resulted in significant elongation of regenerating tibia along the proximodistal axis compared with normal legs. Moreover, our findings indicate that during the regeneration of tibia, the BMP signaling pathway interacts with Dachsous/Fat (Gb’Ds/Gb’Ft) signaling and dachshund (Gb’dac) to re-establish positional information and regulate determination of leg size. Based on these observations, we discuss possible roles for Gb’mad in the distal patterning and intercalation processes during leg regeneration in Gryllus bimaculatus. John Wiley & Sons Japanese Society of Developmental Biologists 2018-07-24 eng journal article AM 343254 https://tokushima-u.repo.nii.ac.jp/records/2009328 https://doi.org/10.1111/dgd.12560 10.1111/dgd.12560 1440169X Development, Growth & Differentiation 60 6 377 386 https://tokushima-u.repo.nii.ac.jp/record/2009328/files/dgd_60_6_377.pdf application/pdf 372 KB 2021-11-05