2026-03-09T08:36:02Z https://tokushima-u.repo.nii.ac.jp/oai

oai:tokushima-u.repo.nii.ac.jp:02009347 2025-04-28T06:26:08Z 1713853213384:1713853295607

Conversion from epithelial to partial-EMT phenotype by Fusobacterium nucleatum infection promotes invasion of oral cancer cells 卲, 文華 ショウ, ブンカ Shao, Wenhua 卲 ショウ Shao 文華 ブンカ Wenhua 50596913 藤原, 奈津美 フジワラ, ナツミ Fujiwara, Natsumi 藤原 フジワラ Fujiwara 奈津美 ナツミ Natsumi 80464353 毛利, 安宏 モウリ, ヤスヒロ Mouri, Yasuhiro 毛利 モウリ Mouri 安宏 ヤスヒロ Yasuhiro キソダ, サトル キソダ, サトル Kisoda, Satoru ヨシダ, カヨ ヨシダ, カヨ Yoshida, Kayo 60363157 吉田, 賀弥 ヨシダ, カヤ Yoshida, Kaya 吉田 ヨシダ Yoshida 賀弥 カヤ Kaya 60284303 湯本, 浩通 ユモト, ヒロミチ Yumoto, Hiromichi 湯本 ユモト Yumoto 浩通 ヒロミチ Hiromichi 90214121 尾崎, 和美 オザキ, カズミ Ozaki, Kazumi 尾崎 オザキ Ozaki 和美 カズミ Kazumi 60314879 石丸, 直澄 イシマル, ナオズミ Ishimaru, Naozumi 石丸 イシマル Ishimaru 直澄 ナオズミ Naozumi 50314753 工藤, 保誠 クドウ, ヤスセイ Kudo, Yasusei 工藤 クドウ Kudo 保誠 ヤスセイ Yasusei open access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The ability of cancer cells to undergo partial-epithelial mesenchymal transition (p-EMT), rather than complete EMT, poses a higher metastatic risk. Although Fusobacterium nucleatum mainly inhabits in oral cavity, attention has been focused on the F. nucleatum involvement in colorectal cancer development. Here we examined the p-EMT regulation by F. nucleatum in oral squamous cell carcinoma (OSCC) cells. We cultured OSCC cells with epithelial, p-EMT or EMT phenotype with live or heat-inactivated F. nucleatum. Expression of the genes involved in epithelial differentiation, p-EMT and EMT were examined in OSCC cells after co-culture with F. nucleatum by qPCR. Cell growth and invasion of OSCC cells were also examined. Both live and heat-inactivated F. nucleatum upregulated the expression of p-EMT-related genes in OSCC cells with epithelial phenotype, but not with p-EMT or EMT phenotype. Moreover, F. nucleatum promoted invasion of OSCC cells with epithelial phenotype. Co-culture with other strains of bacteria other than Porphyromonas gingivalis did not alter p-EMT-related genes in OSCC cells with epithelial phenotype. F. nucleatum infection may convert epithelial to p-EMT phenotype via altering gene expression in OSCC. Oral hygiene managements against F. nucleatum infection may contribute to reduce the risk for an increase in metastatic ability of OSCC. Springer Nature 2021-07-22 eng journal article VoR 377325 https://tokushima-u.repo.nii.ac.jp/records/2009347 https://doi.org/10.1038/s41598-021-94384-1 10.1038/s41598-021-94384-1 20452322 Scientific Reports 11 14943 https://tokushima-u.repo.nii.ac.jp/record/2009347/files/srep_11_14943.pdf application/pdf 17.4 MB 2021-11-09