2026-03-10T02:58:14Z https://tokushima-u.repo.nii.ac.jp/oai

oai:tokushima-u.repo.nii.ac.jp:02011711 2025-04-28T06:46:46Z 1713853213384:1713853295607

Combinatorial expression of ebony and tan generates body color variation from nymph through adult stages in the cricket, Gryllus bimaculatus Role of ebony and tan in cricket body color 井上, 慎太郎 イノウエ, シンタロウ Inoue, Shintaro 井上 イノウエ Inoue 慎太郎 シンタロウ Shintaro 30709481 渡辺, 崇人 ワタナベ, タカヒト Watanabe, Takahito 渡辺 ワタナベ Watanabe 崇人 タカヒト Takahito ハマグチ, タイキ ハマグチ, タイキ Hamaguchi, Taiki 石丸, 善康 イシマル, ヨシヤス Ishimaru, Yoshiyasu 石丸 イシマル Ishimaru 善康 ヨシヤス Yoshiyasu 宮脇, 克行 ミヤワキ, カツユキ Miyawaki, Katsuyuki 宮脇 ミヤワキ Miyawaki 克行 カツユキ Katsuyuki 二川, 健 ニカワ, タケシ Nikawa, Takeshi 二川 ニカワ Nikawa 健 タケシ Takeshi 髙橋, 章 タカハシ, アキラ Takahashi, Akira 髙橋 タカハシ Takahashi 章 アキラ Akira 野地, 澄晴 ノジ, スミハレ Noji, Sumihare 野地 ノジ Noji 澄晴 スミハレ Sumihare 三戸, 太郎 ミト, タロウ Mito, Taro 三戸 ミト Mito 太郎 タロウ Taro open access This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Insect body colors and patterns change markedly during development in some species as they adapt to their surroundings. The contribution of melanin and sclerotin pigments, both of which are synthesized from dopamine, to cuticle tanning has been well studied. Nevertheless, little is known about how insects alter their body color patterns. To investigate this mechanism, the cricket Gryllus bimaculatus, whose body color patterns change during postembryonic development, was used as a model in this study. We focused on the ebony and tan genes, which encode enzymes that catalyze the synthesis and degradation, respectively, of the precursor of yellow sclerotin N-β-alanyl dopamine (NBAD). Expression of the G. bimaculatus (Gb) ebony and tan transcripts tended to be elevated just after hatching and the molting period. We found that dynamic alterations in the combined expression levels of Gb’ebony and Gb’tan correlated with the body color transition from the nymphal stages to the adult. The body color of Gb’ebony knockout mutants generated by CRISPR/Cas9 systemically darkened. Meanwhile, Gb’tan knockout mutants displayed a yellow color in certain areas and stages. The phenotypes of the Gb’ebony and Gb’tan mutants probably result from an over-production of melanin and yellow sclerotin NBAD, respectively. Overall, stage-specific body color patterns in the postembryonic stages of the cricket are governed by the combinatorial expression of Gb’ebony and Gb’tan. Our findings provide insights into the mechanism by which insects evolve adaptive body coloration at each developmental stage. PLOS 2023-05-18 eng journal article VoR 399815 https://tokushima-u.repo.nii.ac.jp/records/2011711 https://doi.org/10.1371/journal.pone.0285934 10.1371/journal.pone.0285934 19326203 PLOS ONE 18 5 e0285934 https://tokushima-u.repo.nii.ac.jp/record/2011711/files/pone_18_5_e0285934.pdf application/pdf 3.57 MB 2024-06-25