2026-03-10T18:45:22Z https://tokushima-u.repo.nii.ac.jp/oai

oai:tokushima-u.repo.nii.ac.jp:02011759 2025-04-28T06:29:26Z 1713853213384:1713853295607

Effects of ferulic acid combined with light irradiation on deoxynivalenol and its production in Fusarium graminearum 白井, 昭博 シライ, アキヒロ Shirai, Akihiro 白井 シライ Shirai 昭博 アキヒロ Akihiro タナカ, アミ タナカ, アミ Tanaka, Ami open access © 2024. This manuscript version is made available under the CC-BY-NC-ND 4.0 license https://creativecommons.org/licenses/by-nc-nd/4.0/ Deoxynivalenol Ferulic acid Light irradiation Cytotoxicity Antifungal assay This study aimed to investigate the effects of ferulic acid (FA), a natural phenolic phytochemical, in combination with light irradiation at three wavelengths (365, 385 and 405 nm) on the concentration and toxicity of deoxynivalenol (DON), a mycotoxin produced by Fusarium graminearum. Moreover, this study examined the influence of the combination treatment on DON production in the cultured fungus. FA activated by light at a peak wavelength of 365 nm exhibited the most effective decrease in DON concentration of the tested wavelengths; a residual DON ratio of 0.23 at 24 h exposure was observed, compared with the initial concentration. The reduction in DON using 365-nm light was dependent on the concentration of FA, with a good correlation (r2=0.979) between the rate constants of DON decrease and FA concentration, which was confirmed by a pseudo-first-order kinetics analysis of the photoreaction with different FA concentrations (50 to 400 mg/L) for 3 h. The viability of HepG2 cells increased by 56.7% following in vitro treatment with a mixture containing the photoproducts obtained after treatment with 20 mg/L DON and 200 mg/L FA under 365-nm irradiation for 6 h. These results suggested that the photoreaction of FA under 365-nm irradiation induces the detoxification of DON through degradation or modification of DON. The antifungal effects of the combination (FA and 365-nm light) on F. graminearum were investigated. Conidia treated with the combination did not show additive or synergistic inhibition of fungal biomass and DON production in 7-day cultivated fungal samples compared with samples after single treatment. However, successive treatment, composed of 90 min irradiation at 365 nm and then treatment with 200 mg/L FA for 90 min in the dark, suppressed fungal growth and DON yield to 70% and 25% of the untreated sample level, respectively. This photo-technology involving the two treatment methods of 365-nm irradiation and FA addition as a food-grade phenolic acid in combination or successively, can aid in developing alternative approaches to eliminate fungal contaminants in the fields of environmental water and agriculture. However, further research is required to explore the underlying mechanisms of DON decontamination and its biosynthesis in F. graminearum. Elsevier 2024-02-10 eng journal article AM 405079 https://tokushima-u.repo.nii.ac.jp/records/2011759 https://doi.org/10.1016/j.funbio.2024.02.003 10.1016/j.funbio.2024.02.003 18786162 18786146 AA12469187 Fungal Biology 128 2 1684 1690 https://tokushima-u.repo.nii.ac.jp/record/2011759/files/funbio_128_2_1684.pdf application/pdf 572 KB 2025-02-10