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Effect of Kampo medicine “Dai-kenchu-to” on microbiome in the intestine of the rats with fast stress 80448331 吉川, 幸造 ヨシカワ, コウゾウ Yoshikawa, Kouzou 吉川 ヨシカワ Yoshikawa 幸造 コウゾウ Kouzou 10216070 島田, 光生 シマダ, ミツオ Shimada, Mitsuo 島田 シマダ Shimada 光生 ミツオ Mitsuo 桑原, 知巳 クワハラ, トモミ Kuwahara, Tomomi ヒラカワ, ヒデキ ヒラカワ, ヒデキ Hirakawa, Hideki 30335814 栗田, 信浩 クリタ, ノブヒロ Kurita, Nobuhiro 栗田 クリタ Kurita 信浩 ノブヒロ Nobuhiro サトウ, ヒロヒコ サトウ, ヒロヒコ Sato, Hirohiko 30304801 宇都宮, 徹 ウツノミヤ, トオル Utsunomiya, Toru 宇都宮 ウツノミヤ Utsunomiya 徹 トオル Toru 00380022 岩田, 貴 イワタ, タカシ Iwata, Takashi 岩田 イワタ Iwata 貴 タカシ Takashi 40448329 宮谷, 知彦 ミヤタニ, トモヒコ Miyatani, Tomohiko 宮谷 ミヤタニ Miyatani 知彦 トモヒコ Tomohiko 30467815 東島, 潤 ヒガシジマ, ジュン Higashijima, Jun 東島 ヒガシジマ Higashijima 潤 ジュン Jun 10548738 柏原, 秀也 カシハラ, ヒデヤ Kashihara, Hideya 柏原 カシハラ Kashihara 秀也 ヒデヤ Hideya 70582823 髙須, 千絵 タカス, チエ Takasu, Chie 髙須 タカス Takasu 千絵 チエ Chie マツモト, ノリコ マツモト, ノリコ Matsumoto, Noriko ナカヤマ イマオウジ, ハルユキ ナカヤマ イマオウジ, ハルユキ Nakayama-Imaohji, Haruyuki open access Daikenchuto microbiome fast stress [Purpose] Diversity of gut microbiome has been recently reported to be lost in inflammatory bowel disease. We have previously reported that the Dai-kenchu-to (DKT) prevented the bacterial translocation through suppression of cytokine and apoptosis in rat’s fast stress model. The aim of this study was to evaluate the effect of DKT on maintenance of microbial diversity in rat’s intestine with inflammation. [Method] Wister rats were received the fast stress for 5 days. In DKT group, rats were administered with DKT (300 mg/kg/day) during the fast stress (DKT-group). The gut microbiomes were analyzed at before- and after- fast stress, and the effect of DKT for on microbial diversities of the gut were evaluated by the PCR-clone library method targeting the 16 S ribosomal RNA gene. [Result] In Control-group, Erysipelotrichaceae increased to 86% in after fast stress, OTU of before-fast stress was 111 and after fast stress was only 9 (changing rate : 58%). The diversity of microbiome was severely decreased. On the other hand, in DKT-group, diversity of microbiome was kept after fast stress (Lachnospiraceae : Ruminococcaceae : Coriobacteriales 54%, 22%, 5%), Operational taxonomic units of before fast stress was 52 and after fast stress was 55 (changing rate : 6%). Family Lachnospiraceae which includes butyrate-producing Clostridia (Clostridium IV and XIVa). [Conclusion] DKT prevented the reduction of diversity of microbiome in rat’s fast stress model. Our data suggested the new anti-inflammatory mechanism of DKT through gut microbiome. 2013-08 eng journal article VoR 280901 https://tokushima-u.repo.nii.ac.jp/records/2002520 13431420 AA11166929 The journal of medical investigation : JMI 60 3-4 221 227 https://tokushima-u.repo.nii.ac.jp/record/2002520/files/LID201507305002.pdf application/pdf 855 KB 2015-07-30