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oai:tokushima-u.repo.nii.ac.jp:02002629 2025-04-28T07:21:49Z 1713853213384:1713853296295:1716267876683:1716268551628

Cyclopamine Decreased the Expression of Sonic Hedgehog and its Downstream Genes in Colon Cancer Stem Cells Cyclopamineは大腸癌癌幹細胞のソニック・ヘッジホッグとその下流遺伝子発現を抑制する Sonic Hedgehog Downstream Genes in Cancer Stem Cells バトサイハン, バトエルデネ バトサイハン, バトエルデネ Batsaikhan, Bat-Erdene 吉川, 幸造 ヨシカワ, コウゾウ Yoshikawa, Kouzou 吉川 ヨシカワ Yoshikawa 幸造 コウゾウ Kouzou 栗田, 信浩 クリタ, ノブヒロ Kurita, Nobuhiro 栗田 クリタ Kurita 信浩 ノブヒロ Nobuhiro 岩田, 貴 イワタ, タカシ Iwata, Takashi 岩田 イワタ Iwata 貴 タカシ Takashi 髙須, 千絵 タカス, チエ Takasu, Chie 髙須 タカス Takasu 千絵 チエ Chie 柏原, 秀也 カシハラ, ヒデヤ Kashihara, Hideya 柏原 カシハラ Kashihara 秀也 ヒデヤ Hideya 島田, 光生 シマダ, ミツオ Shimada, Mitsuo 島田 シマダ Shimada 光生 ミツオ Mitsuo open access Cancer stem-like cells epithelial-mesenchymal transition cancer cells sonic hedgehog Backround: Most solid cancers including colon cancer are believed to be initiated from and maintained by cancer stem cells (CSCs), that are responsible for treatment resistance, resulting in tumor relapse. The aim of this study was to clarify the possible role of the Sonic Hedgehog (Shh) signaling pathway in the regulation of cancer stem cells. Materials and Methods: The HCT-116 cell line was cultured with fetal bovine serum in RPMI-1640 medium and its sphere was grown in serum-free non-adherent culture. Gene expressions were analyzed by quantitative real-time polymerase chain reaction (qRT-PCR) from cells treated with and without cyclopamine. Results: HCT-116 sphere-derived cells grown in serum-free, non-adherent culture, showed significantly increased expression of stem cell markers, Shh downstream genes and epithelial-mesenchymal transition (EMT) markers compared to parental cells grown in conventional culture. The expression of stemness markers, Shh downstream genes and EMT markers were higher in cancer spheres than the parental cell line and down-regulated by cyclopamine treatment in a dose-dependent manner. Conclusion: Overall, these findings show that cyclopamine treatment could down-regulate the expression of stemness markers, shh downstream genes and EMT markers on HCT-116 spheres. International Institute of Anticancer Research 2014-11-03 eng doctoral thesis NA 297570 https://tokushima-u.repo.nii.ac.jp/records/2002629 https://ar.iiarjournals.org/content/anticanres/34/11/6339.full.pdf 02507005 17917530 AA10625860 AA12440673 Anticancer Research 34 11 6339 6344 甲医第1238号 博士（医学） 2015-03-23 徳島大学 https://tokushima-u.repo.nii.ac.jp/record/2002629/files/LID201505251001.pdf application/pdf 134 KB 2015-05-25 https://tokushima-u.repo.nii.ac.jp/record/2002629/files/k2796_fulltext.pdf application/pdf 229 KB 2022-12-14