2026-03-15T20:24:00Z https://tokushima-u.repo.nii.ac.jp/oai

oai:tokushima-u.repo.nii.ac.jp:02007907 2025-04-28T06:29:30Z 1713853213384:1713853295607

Involvement of dachshund and Distal-less in distal pattern formation of the cricket leg during regeneration 50435525 石丸, 善康 イシマル, ヨシヤス Ishimaru, Yoshiyasu 石丸 イシマル Ishimaru 善康 ヨシヤス Yoshiyasu ナカムラ, タロウ ナカムラ, タロウ Nakamura, Taro バンドウ, テツヤ バンドウ, テツヤ Bando, Tetsuya マツオカ, ユウジ マツオカ, ユウジ Matsuoka, Yuji 00253229 大内, 淑代 オオウチ, ヒデヨ Ohuchi, Hideyo 大内 オオウチ Ohuchi 淑代 ヒデヨ Hideyo 40156211 野地, 澄晴 ノジ, スミハレ Noji, Sumihare 野地 ノジ Noji 澄晴 スミハレ Sumihare 80322254 三戸, 太郎 ミト, タロウ Mito, Taro 三戸 ミト Mito 太郎 タロウ Taro open access This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ Cricket nymphs have the remarkable ability to regenerate a functional leg following amputation, indicating that the regenerating blastemal cells contain information for leg morphology. However, the molecular mechanisms that underlie regeneration of leg patterns remain poorly understood. Here, we analyzed phenotypes of the tibia and tarsus (three tarsomeres) obtained by knockdown with regeneration-dependent RNA interference (rdRNAi) against Gryllus dachshund (Gb'dac) and Distal-less (Gb'Dll). We found that depletion of Gb'Dll mRNA results in loss of the tarsal segments, while rdRNAi against Gb'dac shortens the tibia at the two most distal tarsomeres. These results indicate that Gb'Dll expression is indispensable for formation of the tarsus, while Gb'dac expression is necessary for elongation of the tibia and formation of the most proximal tarsomere. These findings demonstrate that mutual transcriptional regulation between the two is indispensable for formation of the tarsomeres, whereas Gb'dac is involved in determination of tibial size through interaction with Gb'ds/Gb'ft. Springer Nature 2015-02-11 eng journal article VoR 294555 https://tokushima-u.repo.nii.ac.jp/records/2007907 https://doi.org/10.1038/srep08387 10.1038/srep08387 20452322 Scientific Reports 5 8387 https://tokushima-u.repo.nii.ac.jp/record/2007907/files/srep_5_8387.pdf application/pdf 5.94 MB 2020-08-07