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Interaction of Protein Phosphatase 1δ with Nucleophosmin in Human Osteoblastic Cells Protein Phosphatase 1δ with Nucleophosmin 50156379 羽地, 達次 ハネジ, タツジ Haneji, Tatsuji 羽地 ハネジ Haneji 達次 タツジ Tatsuji 20515986 寺町, 順平 テラマチ, ジュンペイ Teramachi, Jumpei 寺町 テラマチ Teramachi 順平 ジュンペイ Jumpei ヒラシマ, カンジ ヒラシマ, カンジ Hirashima, Kanji キムラ, コウジ キムラ, コウジ Kimura, Koji 森本, 景之 モリモト, ヒロユキ Morimoto, Hiroyuki open access This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. protein phosphatase nucleophosmin osteoblast Protein phosphorylation and dephosphorylation has been recognized as an essential mechanism in the regulation of cellular metabolism and function in various tissues. Serine and threonine protein phosphatases (PP) are divided into four categories: PP1, PP2A, PP2B, and PP2C. At least four isoforms of PP1 catalytic subunit in rat, PP1α, PP1γ1, PP1γ2, and PP1δ, were isolated. In the present study, we examined the localization and expression of PP1δ in human osteoblastic Saos-2 cells. Anti-PP1δ antibody recognized a protein present in the nucleolar regions in Saos-2 cells. Cellular fractionation revealed that PP1δ is a 37 kDa protein localized in the nucleolus. Nucleophosmin is a nucleolar phosphoprotein and located mainly in the nucleolus. Staining pattern of nucleophosmin in Saos-2 cells was similar to that of PP1δ. PP1δ and nucleophosmin were specifically stained as dots in the nucleus. Dual fluorescence images revealed that PP1δ and nucleophosmin were localized in the same regions in the nucleolus. Similar distribution patterns of PP1δ and nucleophosmin were observed in osteoblastic MG63 cells. The interaction of PP1δ and nucleophosmin was also shown by immunoprecipitation and Western analysis. These results indicated that PP1δ associate with nucleophosmin directly in the nucleolus and suggested that nucleophosmin is one of the candidate substrate for PP1δ. The Japan Society of Histochemistry and Cytochemistry 2011-11-05 eng journal article VoR 251594 https://tokushima-u.repo.nii.ac.jp/records/2007911 https://doi.org/10.1267/ahc.11041 10.1267/ahc.11041 00445991 13475800 AA00508022 Acta Histochemica et Cytochemica 45 1 1 7 https://tokushima-u.repo.nii.ac.jp/record/2007911/files/ahc_45_1_1.pdf application/pdf 1.32 MB 2020-08-19