2026-03-15T20:11:05Z https://tokushima-u.repo.nii.ac.jp/oai

oai:tokushima-u.repo.nii.ac.jp:02008721 2025-04-28T06:29:29Z 1713853213384:1713853295607

Leg regeneration is epigenetically regulated by histone H3K27 methylation in the cricket Gryllus bimaculatus 濱田, 良真 ハマダ, ヨシマサ Hamada, Yoshimasa 濱田 ハマダ Hamada 良真 ヨシマサ Yoshimasa バンドウ, テツヤ バンドウ, テツヤ Bando, Tetsuya ナカムラ, タロウ ナカムラ, タロウ Nakamura, Taro 50435525 石丸, 善康 イシマル, ヨシヤス Ishimaru, Yoshiyasu 石丸 イシマル Ishimaru 善康 ヨシヤス Yoshiyasu 80322254 三戸, 太郎 ミト, タロウ Mito, Taro 三戸 ミト Mito 太郎 タロウ Taro 40156211 野地, 澄晴 ノジ, スミハレ Noji, Sumihare 野地 ノジ Noji 澄晴 スミハレ Sumihare トミオカ, ケンジ トミオカ, ケンジ Tomioka, Kenji 00253229 大内, 淑代 オオウチ, ヒデヨ Ohuchi, Hideyo 大内 オオウチ Ohuchi 淑代 ヒデヨ Hideyo open access Regeneration Epigenetics Histone H3K27 Gryllus bimaculatus Polycomb Hemimetabolous insects such as the cricket Gryllus bimaculatus regenerate lost tissue parts using blastemal cells, a population of dedifferentiated proliferating cells. The expression of several factors that control epigenetic modification is upregulated in the blastema compared with differentiated tissue, suggesting that epigenetic changes in gene expression might control the differentiation status of blastema cells during regeneration. To clarify the molecular basis of epigenetic regulation during regeneration, we focused on the function of the Gryllus Enhancer of zeste [Gb'E(z)] and Ubiquitously transcribed tetratricopeptide repeat gene on the X chromosome (Gb'Utx) homologues, which regulate methylation and demethylation of histone H3 lysine 27 (H3K27), respectively. Methylated histone H3K27 in the regenerating leg was diminished by Gb'E(z)RNAi and was increased by Gb'UtxRNAi. Regenerated Gb'E(z)RNAi cricket legs exhibited extra leg segment formation between the tibia and tarsus, and regenerated Gb'UtxRNAi cricket legs showed leg joint formation defects in the tarsus. In the Gb'E(z)RNAi regenerating leg, the Gb'dac expression domain expanded in the tarsus. By contrast, in the Gb'UtxRNAi regenerating leg, Gb'Egfr expression in the middle of the tarsus was diminished. These results suggest that regulation of the histone H3K27 methylation state is involved in the repatterning process during leg regeneration among cricket species via the epigenetic regulation of leg patterning gene expression. The Company of Biologists 2015-09-01 eng journal article VoR 303095 https://tokushima-u.repo.nii.ac.jp/records/2008721 https://doi.org/10.1242/dev.122598 10.1242/dev.122598 09501991 14779129 AA10667805 AA12024599 Development 142 17 2916 2927 https://tokushima-u.repo.nii.ac.jp/record/2008721/files/dev_142_17_2916.pdf application/pdf 4.54 MB 2021-03-12