2026-03-10T03:20:49Z https://tokushima-u.repo.nii.ac.jp/oai

oai:tokushima-u.repo.nii.ac.jp:02009444 2025-04-28T06:46:41Z 1713829974766:1713854681789:1716364513618:1716365191274 1713853213384:1713853295607

Effective in vitro differentiation of adipose-derived stem cells into Schwann-like cells with folic acid supplementation Schwann-like cells from ADSCs using folic acid Chen, Shuhai 池本, 哲也 イケモト, テツヤ Ikemoto, Tetsuya 池本 イケモト Ikemoto 哲也 テツヤ Tetsuya 徳永, 卓哉 トクナガ, タクヤ Tokunaga, Takuya 徳永 トクナガ Tokunaga 卓哉 タクヤ Takuya オキカワ, ショウヘイ オキカワ, ショウヘイ Okikawa, Shohei 30846126 宮崎, 克己 ミヤザキ, カツキ Miyazaki, Katsuki 宮崎 ミヤザキ Miyazaki 克己 カツキ Katsuki トクダ, カズノリ トクダ, カズノリ Tokuda, Kazunori 山田, 眞一郎 ヤマダ, シンイチロウ Yamada, Shin-ichiro 山田 ヤマダ Yamada 眞一郎 シンイチロウ Shin-ichiro 齋藤, 裕 サイトウ, ユウ Saitou, Yu 齋藤 サイトウ Saitou 裕 ユウ Yu 居村, 暁 イムラ, サトル Imura, Satoru 居村 イムラ Imura 暁 サトル Satoru 森根, 裕二 モリネ, ユウジ Morine, Yuji 森根 モリネ Morine 裕二 ユウジ Yuji 島田, 光生 シマダ, ミツオ Shimada, Mitsuo 島田 シマダ Shimada 光生 ミツオ Mitsuo open access Adipose-derived stem cell Schwann-like cells Folic acid Nerve growth factor Regenerative medicine Peripheral nerve injury (PNI) after pelvic surgery is a common issue with a significant impact on patients. Autologous nerve grafting is the gold standard treatment for PNI, but this technique cannot be applied to fine nerve fibers in the pelvis. Schwann-like cell (SLC) differentiation is a novel therapeutic strategy for this clinical condition. However, the efficiency of SLC differentiation remains unsatisfactory. We modified an SLC differentiation protocol using adipose-derived stem cells (ADSCs) and folic acid. Morphology, gene expression and secretion of neurotrophic factors were examined to assess the differentiation quality and phenotypic characteristics. Our new modified protocol effectively induced a Schwann cell (SC) phenotype in ADSCs as assessed by morphology and expression of SC markers [S100 calcium-binding protein B (S100B), P < 0.01 ; p75 neurotrophic receptor (p75NTR), P < 0.05]. SLCs produced by the new protocol displayed a repair phenotype with decreased expression of ERBB2 and early growth response protein 2 (EGR2) / KROX20 (P < 0.01). Furthermore, our new protocol enhanced both mRNA expression and secretion of nerve growth factors by SLCs (P < 0.01). This protocol enhanced the SC characteristics and functions of ADSC-derived SLCs. This promising protocol requires further research and may contribute to SC-based nerve regeneration. Tokushima University Faculty of Medicine 2021-08 eng journal article VoR 386671 https://tokushima-u.repo.nii.ac.jp/records/2009444 https://doi.org/10.2152/jmi.68.347 10.2152/jmi.68.347 13496867 13431420 AA11166929 The Journal of Medical Investigation 68 3-4 347 353 https://tokushima-u.repo.nii.ac.jp/record/2009444/files/jmi_68_3-4_347.pdf application/pdf 582 KB 2021-12-03