2026-03-10T02:55:17Z https://tokushima-u.repo.nii.ac.jp/oai

oai:tokushima-u.repo.nii.ac.jp:02011672 2025-04-28T06:27:43Z 1713853213384:1713853295607

Recycling of memory B cells between germinal center and lymph node subcapsular sinus supports affinity maturation to antigenic drift Zhang, Yang Garcia-Ibanez, Laura Ulbricht, Carolin Lok, Laurence S. C. Pike, Jeremy A. Mueller-Winkler, Jennifer Dennison, Thomas W. Ferdinand, John R. Burnett, Cameron J. M. Yam-Puc, Juan C. Zhang, Lingling Maqueda Alfaro, Raul 高浜, 洋介 タカハマ, ヨウスケ Takahama, Yousuke 高浜 タカハマ Takahama 洋介 ヨウスケ Yousuke 00596588 大東, いずみ オオヒガシ, イズミ Ohigashi, Izumi 大東 オオヒガシ Ohigashi いずみ イズミ Izumi Brown, Geoffrey クロサキ, トモヒロ クロサキ, トモヒロ Kurosaki, Tomohiro Tybulewicz, Victor L. J. Rot, Antal Hauser, Anja E. Clatworthy, Menna R. Toellner, Kai-Michael open access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. Infection or vaccination leads to the development of germinal centers (GC) where B cells evolve high affinity antigen receptors, eventually producing antibody-forming plasma cells or memory B cells. Here we follow the migratory pathways of B cells emerging from germinal centers (BEM) and find that many BEM cells migrate into the lymph node subcapsular sinus (SCS) guided by sphingosine-1-phosphate (S1P). From the SCS, BEM cells may exit the lymph node to enter distant tissues, while some BEM cells interact with and take up antigen from SCS macrophages, followed by CCL21-guided return towards the GC. Disruption of local CCL21 gradients inhibits the recycling of BEM cells and results in less efficient adaption to antigenic variation. Our findings thus suggest that the recycling of antigen variant-specific BEM cells and transport of antigen back to GC may support affinity maturation to antigenic drift. Springer Nature 2022-05-05 eng journal article VoR 394963 https://tokushima-u.repo.nii.ac.jp/records/2011672 https://doi.org/10.1038/s41467-022-29978-y 10.1038/s41467-022-29978-y 20411723 AA12645905 Nature Communications 13 2460 https://tokushima-u.repo.nii.ac.jp/record/2011672/files/ncomms_13_2460.pdf application/pdf 8.32 MB 2024-04-01