2026-03-05T07:58:02Z https://tokushima-u.repo.nii.ac.jp/oai

oai:tokushima-u.repo.nii.ac.jp:02013296 2025-06-11T08:06:17Z 1713829974766:1713854681789:1745467575633:1745467619908 1713853213384:1713853295607

Melanoma antigen is a vaccine candidate against Meth A sarcoma Melanoma antigen is a vaccine candidate antigen 90803536 中本, 晶子 ナカモト, アキコ Nakamoto, Akiko 中本 ナカモト Nakamoto 晶子 アキコ Akiko Ohashi, Haruka Tanaka, Yuko Yamamoto, Mako 40722533 中本, 真理子 ナカモト, マリコ Nakamoto, Mariko 中本 ナカモト Nakamoto 真理子 マリコ Mariko 10512121 首藤, 恵泉 シュトウ, エミ Shuto, Emi 首藤 シュトウ Shuto 恵泉 エミ Emi 40274196 酒井, 徹 サカイ, トオル Sakai, Tohru 酒井 サカイ Sakai 徹 トオル Tohru open access Meth A CD8+ cells melanoma antigen vaccine Sarcoma Meth A is widely used in the field of immunology and oncology study. We found that a mutant cell line, Meth A (mMeth A), was rejected in an in vivo subcutaneous challenge in BALB / c mice. mMeth A cells were not rejected in athymic BALB / c-nu / nu mice and CD8+ cell-depleted BALB / c mice, suggesting that CD8+ cells are required for rejection of mMeth A cells. Microarray analysis showed that melanoma antigen (MAg) was one of the most elevated genes in mMeth A cells. Indeed, quantitative gene expression analysis showed that the expression level of MAg in mMeth A cells was one hundred-times higher than that in Meth A cells. We constructed two types of expression vector coding the MAg gene sequence corresponding to 788–1257 and 1611-2043 and immunized mice with these genes by intramuscular injection. Immunization of a plasmid expressing the Mag 788-1257 gene protected the mice from in vivo Meth A challenge as evaluated by tumor volume and survival rate. The results reveal that MAg is a potential vaccine candidate antigen against Meth A tumors. Tokushima University Faculty of Medicine 2025-02 eng journal article VoR 433634 https://tokushima-u.repo.nii.ac.jp/records/2013296 https://doi.org/10.2152/jmi.72.161 10.2152/jmi.72.161 13496867 13431420 AA11166929 The Journal of Medical Investigation 72 1-2 161 166 https://tokushima-u.repo.nii.ac.jp/record/2013296/files/jmi_72_1-2_161.pdf application/pdf 1.9 MB 2025-06-11