2026-03-05T08:00:53Z https://tokushima-u.repo.nii.ac.jp/oai

oai:tokushima-u.repo.nii.ac.jp:02002629 2025-04-28T07:21:49Z 1713853213384:1713853296295:1716267876683:1716268551628

Cyclopamineは大腸癌癌幹細胞のソニック・ヘッジホッグとその下流遺伝子発現を抑制する Sonic Hedgehog Downstream Genes in Cancer Stem Cells Cyclopamine Decreased the Expression of Sonic Hedgehog and its Downstream Genes in Colon Cancer Stem Cells バトサイハン, バトエルデネ バトサイハン, バトエルデネ Batsaikhan, Bat-Erdene 吉川, 幸造 294 ヨシカワ, コウゾウ 80448331 Yoshikawa, Kouzou 栗田, 信浩 1154 クリタ, ノブヒロ 30335814 Kurita, Nobuhiro 岩田, 貴 1259 イワタ, タカシ 82912/profile-ja.html Iwata, Takashi 00380022 髙須, 千絵 517 タカス, チエ 229092/profile-ja.html Takasu, Chie 70582823 柏原, 秀也 791 カシハラ, ヒデヤ 294061/profile-ja.html Kashihara, Hideya 10548738 島田, 光生 1253 シマダ, ミツオ 82712/profile-ja.html Shimada, Mitsuo 10216070 Cancer stem-like cells epithelial-mesenchymal transition cancer cells sonic hedgehog Backround: Most solid cancers including colon cancer are believed to be initiated from and maintained by cancer stem cells (CSCs), that are responsible for treatment resistance, resulting in tumor relapse. The aim of this study was to clarify the possible role of the Sonic Hedgehog (Shh) signaling pathway in the regulation of cancer stem cells. Materials and Methods: The HCT-116 cell line was cultured with fetal bovine serum in RPMI-1640 medium and its sphere was grown in serum-free non-adherent culture. Gene expressions were analyzed by quantitative real-time polymerase chain reaction (qRT-PCR) from cells treated with and without cyclopamine. Results: HCT-116 sphere-derived cells grown in serum-free, non-adherent culture, showed significantly increased expression of stem cell markers, Shh downstream genes and epithelial-mesenchymal transition (EMT) markers compared to parental cells grown in conventional culture. The expression of stemness markers, Shh downstream genes and EMT markers were higher in cancer spheres than the parental cell line and down-regulated by cyclopamine treatment in a dose-dependent manner. Conclusion: Overall, these findings show that cyclopamine treatment could down-regulate the expression of stemness markers, shh downstream genes and EMT markers on HCT-116 spheres. doctoral thesis International Institute of Anticancer Research 2014-11-03 NA application/pdf application/pdf Anticancer Research 11 34 6339 6344 02507005 17917530 AA10625860 AA12440673 https://tokushima-u.repo.nii.ac.jp/record/2002629/files/LID201505251001.pdf https://tokushima-u.repo.nii.ac.jp/record/2002629/files/k2796_fulltext.pdf https://tokushima-u.repo.nii.ac.jp/records/2002629 eng