{"created":"2024-09-24T00:31:04.989145+00:00","id":2000187,"links":{},"metadata":{"_buckets":{"deposit":"878cef62-0f05-4221-856e-cf424b294a9b"},"_deposit":{"created_by":52,"id":"2000187","owner":"52","owners":[52],"pid":{"revision_id":0,"type":"depid","value":"2000187"},"status":"published"},"_oai":{"id":"oai:tokushima-u.repo.nii.ac.jp:02000187","sets":["1713853213384:1713853295607"]},"author_link":["301"],"control_number":"2000187","item_10001_biblio_info_7":{"attribute_name":"bibliographic_information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2024-03-05","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3","bibliographicPageStart":"100510","bibliographicVolumeNumber":"4","bibliographic_titles":[{"bibliographic_title":"Cell Genomics","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"CRISPR-Cas9 short guide RNA (sgRNA) library screening is a powerful approach to understand the molecular mechanisms of biological phenomena. However, its in vivo application is currently limited. Here, we developed our previously established in vitro revival screening method into an in vivo one to identify factors involved in spermatogenesis integrity by utilizing sperm capacitation as an indicator. By introducing an sgRNA library into testicular cells, we successfully pinpointed the retinal degeneration 3 (Rd3) gene as a significant factor in spermatogenesis. Single-cell RNA sequencing (scRNA-seq) analysis highlighted the high expression of Rd3 in round spermatids, and proteomics analysis indicated that Rd3 interacts with mitochondria. To search for cell-type-specific signaling pathways based on scRNA-seq and proteomics analyses, we developed a computational tool, Hub-Explorer. Through this, we discovered that Rd3 modulates oxidative stress by regulating mitochondrial distribution upon ciliogenesis induction. Collectively, our screening system provides a valuable in vivo approach to decipher molecular mechanisms in biological processes.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier","subitem_publisher_language":"en"}]},"item_10001_rights_15":{"attribute_name":"item_10001_rights_15","attribute_value_mlt":[{"subitem_rights":"This is an open access article unde rthe CC BY-NC-ND license.","subitem_rights_language":"en"}]},"item_10001_source_id_9":{"attribute_name":"収録物ID","attribute_value_mlt":[{"subitem_source_identifier":"2666979X","subitem_source_identifier_type":"EISSN"}]},"item_10001_version_type_20":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_1715043197608":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_1722929371688":{"attribute_name":"item_1722929371688","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_language":"ja","subitem_relation_name_text":"10.1016/j.xgen.2024.100510"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1016/j.xgen.2024.100510","subitem_relation_type_select":"DOI"}}]},"item_1723180141928":{"attribute_name":"item_1723180141928","attribute_value_mlt":[{"subitem_identifier_type":"URI","subitem_identifier_uri":"409523"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Noguchi, Yuki","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Onodera, Yasuhito","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Miyamoto, Tatsuo","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Maruoka, Masahiro","creatorNameLang":"en"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"小迫, 英尊","creatorNameLang":"ja"},{"creatorName":"コサコ, ヒデタカ","creatorNameLang":"ja-Kana"},{"creatorName":"Kosako, Hidetaka","creatorNameLang":"en"}],"familyNames":[{"familyName":"小迫","familyNameLang":"ja"},{"familyName":"コサコ","familyNameLang":"ja-Kana"},{"familyName":"Kosako","familyNameLang":"en"}],"givenNames":[{"givenName":"英尊","givenNameLang":"ja"},{"givenName":"ヒデタカ","givenNameLang":"ja-Kana"},{"givenName":"Hidetaka","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"301","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"172252/profile-ja.html","nameIdentifierScheme":"徳島大学 教育研究者総覧","nameIdentifierURI":"http://pub2.db.tokushima-u.ac.jp/ERD/person/172252/profile-ja.html"},{"nameIdentifier":"10291171","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://nrid.nii.ac.jp/ja/search/?qm=10291171"}]},{"creatorNames":[{"creatorName":"Suzuki, Jun","creatorNameLang":"en"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2024-01-01"}],"displaytype":"detail","filename":"cgen_4_3_100510.pdf","filesize":[{"value":"7.1 MB"}],"format":"application/pdf","licensetype":"license_5","mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://tokushima-u.repo.nii.ac.jp/record/2000187/files/cgen_4_3_100510.pdf"},"version_id":"9e953d90-70e0-4e88-b43f-c99ddee7d830"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"item_resource_type","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"In vivo CRISPR screening directly targeting testicular cells","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"In vivo CRISPR screening directly targeting testicular cells","subitem_title_language":"en"}]},"item_type_id":"40001","owner":"52","path":["1713853295607"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2024-10-21"},"publish_date":"2024-10-21","publish_status":"0","recid":"2000187","relation_version_is_last":true,"title":["In vivo CRISPR screening directly targeting testicular cells"],"weko_creator_id":"52","weko_shared_id":-1},"updated":"2025-01-29T06:55:07.864766+00:00"}