| アイテムタイプ |
文献 / Documents(1) |
| 公開日 |
2011-11-21 |
| アクセス権 |
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アクセス権 |
open access |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 出版タイプ |
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出版タイプ |
VoR |
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出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
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タイトル |
Overexpression of wild-type Akt1 promoted insulin-stimulated p70S6 kinase (p70S6K) activity and affected GSK3 beta regulation, but did not promote insulin-stimulated GLUT4 translocation or glucose transport in L6 myotubes |
| 著者 |
ノダ, サトシ
岸, 和弘
湯浅, 智之
WEKO
1115
e-Rad_Researcher
50304556
| ja |
湯浅, 智之
湯浅, 智之
ISNI
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| ja-Kana |
ユアサ, トモユキ
ユアサ, トモユキ
|
| en |
Yuasa, Tomoyuki
Yuasa, Tomoyuki
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Search repository
ハヤシ, ヒデキ
オオニシ, テツオ
ミヤタ, イクコ
西谷, 弘
蛯名, 洋介
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| 抄録 |
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内容記述 |
We have developed a simple, direct and sensitive method to detect GLUT4 on the cell surface. Using this system, we found that PI3-kinase plays a key role in the signaling pathway of insulin-stimulated GLUT4 translocation. One of the down stream effectors of PI3-kinase is serine-threonine kinase Akt (protein kinase B, RAK-PK), but the involvement of Akt in insulin-stimulated GLUT4 translocation is controversial. To investigate whether Akt1 regulates insulin-stimulated GLUT4 translocation and glucose uptake in L6 myotubes, we established L6 myotubes stably expressing c-myc epitope-tagged GLUT4 (GLUT4myc) and mouse wild type (WT) Akt1. We found that overexpression of WT Akt1 promoted insulin-stimulated p70S6 kinase (p70S6K) activity and increased the basal activity of GSK3β, but did not promote insulin-stimulated GLUT4translocation or glucose uptake. These data supported the result that Akt is not a main signaling molecule to transmit the signal of insulin-stimulated GLUT4 translocation or glucose uptake from insulin-activated PI3-kinase. |
| キーワード |
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主題 |
AKT |
| キーワード |
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主題 |
p70S6 kinase |
| キーワード |
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主題 |
GSK3β |
| キーワード |
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主題 |
GLUT4 |
| 書誌情報 |
en : The journal of medical investigation : JMI
巻 47,
号 1-2,
p. 47-55,
発行日 2000
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| 収録物ID |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
13431420 |
| 収録物ID |
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収録物識別子タイプ |
NCID |
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収録物識別子 |
AA11166929 |
| 備考 |
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|
値 |
データ元 : http://medical.med.tokushima-u.ac.jp/jmi/ |
| EID |
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識別子 |
66779 |
| 言語 |
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言語 |
eng |
LID201111212007.pdf (198 KB)
