{"created":"2024-10-09T10:27:27.656854+00:00","id":2001062,"links":{},"metadata":{"_buckets":{"deposit":"d9206a98-8af3-444e-9655-509177a76de2"},"_deposit":{"created_by":7,"id":"2001062","owners":[7],"pid":{"revision_id":0,"type":"depid","value":"2001062"},"status":"published"},"_oai":{"id":"oai:tokushima-u.repo.nii.ac.jp:02001062","sets":["1713829974766:1713854680413:1716363998031","1713853213384:1713853297799"]},"author_link":["180","61","118"],"item_10001_alternative_title_1":{"attribute_name":"タイトル別表記","attribute_value_mlt":[{"subitem_alternative_title":"Development of Biosensors Using Hyperthermophilic Enzymes as an Element:Development of Biosensors Using Hyperthermophilic Enzymes as an Element:Functional Analysis of Polyamine Metabolizing Enzymes and Development for EnzymeElectrode Sensor Using D-Proline Dehydrogenases","subitem_alternative_title_language":"en"}]},"item_10001_biblio_info_7":{"attribute_name":"bibliographic_information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2005-03","bibliographicIssueDateType":"Issued"},"bibliographicVolumeNumber":"50","bibliographic_titles":[{"bibliographic_title":"徳島大学工学部研究報告","bibliographic_titleLang":"ja"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"An amperometric enzyme sensor give us higher sensitivity and specificity for the substrate\ndetermination. In spite of advantages of enzyme sensor, many enzymes so far found have been too\nlabile to use as biosensor elements in artificial circumstances for a longer period.\nHyperthermophiles, which can grow above 90℃, have been known to produce much more stable\nenzymes under various artificial conditions. In this work, we carried out screening, biochemical\ncharacterization and improvement of production for hyperthermostable enzymes which are more useful\nas the elements in the biosensors. We focused on the polyamines as one of the substrates of\nbiosensors. Polyamines have been known to play many important roles in cell proliferation,\ndifferentiation and transformation. The concentration of the polyamines together with their acetyl\nconjugates remarkably increases in the biological fluids and the affected tissues of cancer patients.\nTherefore, their polyamines are listed as tumor markers. Gas and ion chromatographies have been\nso far used for polyamine determination, but have some problems from the aspects of high sensitivity\nand easy operation. Thus, we here developed biosensors using hyperthermostable enzymes for\npolyamine determination. Such enzyme sensors are more useful for the simple and rapid\ndetermination of polyamines and application for clinical analysis and food analysis In addition, we\ntried the construction of biosensor using the hyperthermophilic enzyme, D-Proline dehydrogenase.\nAs the results, we found the thermostable agmatinase and spermidine dehydrogenase in\nhyperthermophiles, Pyrococcus horikoshii and Sulfolobus tokodaii, respectively. We succeeded the\nconstruction of novel amperometric sensor for D-proline determination using D-Proline dehydrogenase\nderived from Pyrobaculum islandicum.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_source_id_9":{"attribute_name":"収録物ID","attribute_value_mlt":[{"subitem_source_identifier":"03715949","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"AA1221470X","subitem_source_identifier_type":"NCID"}]},"item_10001_version_type_20":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_1715043197608":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access"}]},"item_1723180141928":{"attribute_name":"item_1723180141928","attribute_value_mlt":[{"subitem_identifier_type":"URI","subitem_identifier_uri":"121859"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"郷田, 秀一郎","creatorNameLang":"ja"},{"creatorName":"ゴウダ, シュウイチロウ","creatorNameLang":"ja-Kana"},{"creatorName":"Goda, Shuichiro","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"薮谷, 智規","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"180","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"櫻庭, 春彦","creatorNameLang":"ja"},{"creatorName":"サクラバ, ハルヒコ","creatorNameLang":"ja-Kana"},{"creatorName":"Sakuraba, Haruhiko","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"本仲, 純子","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"61","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"大島, 敏久","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"118","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2011-03-03"}],"displaytype":"detail","filename":"EID121859.pdf","filesize":[{"value":"350 KB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://tokushima-u.repo.nii.ac.jp/record/2001062/files/EID121859.pdf"},"version_id":"1619aa80-9147-4ad6-ac56-397288f43e36"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"enzyme sensor","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"hyperthermophile","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"dehydrogenase","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"polyamine","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"item_resource_type","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"超好熱菌酵素を素子とするバイオセンサーの開発 : ポリアミン関連酵素の機能解析とD-プロリン脱水素酵素機能電極センサーの開発","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"超好熱菌酵素を素子とするバイオセンサーの開発 : ポリアミン関連酵素の機能解析とD-プロリン脱水素酵素機能電極センサーの開発","subitem_title_language":"ja"},{"subitem_title":"チョウコウネツキン コウソ オ ソシ ト スル バイオ センサー ノ カイハツ : ポリアミン カンレン コウソ ノ キノウ カイセキ ト D-プロリン ダツスイソ コウソ キノウ デンキョク センサー ノ カイハツ","subitem_title_language":"ja-Kana"}]},"item_type_id":"40001","owner":"7","path":["1713853297799","1716363998031"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2011-03-03"},"publish_date":"2011-03-03","publish_status":"0","recid":"2001062","relation_version_is_last":true,"title":["超好熱菌酵素を素子とするバイオセンサーの開発 : ポリアミン関連酵素の機能解析とD-プロリン脱水素酵素機能電極センサーの開発"],"weko_creator_id":"7","weko_shared_id":-1},"updated":"2024-10-09T10:27:35.708828+00:00"}