{"created":"2024-10-09T10:31:35.990769+00:00","id":2001118,"links":{},"metadata":{"_buckets":{"deposit":"2603bd8c-5d57-4093-8574-e722bddd5e76"},"_deposit":{"created_by":7,"id":"2001118","owners":[7],"pid":{"revision_id":0,"type":"depid","value":"2001118"},"status":"published"},"_oai":{"id":"oai:tokushima-u.repo.nii.ac.jp:02001118","sets":["1713829974766:1713833414474:1716344317782","1713853213384:1713853297799"]},"author_link":["1242"],"item_10001_biblio_info_7":{"attribute_name":"bibliographic_information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2008-12","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"104","bibliographicPageStart":"97","bibliographicVolumeNumber":"22","bibliographic_titles":[{"bibliographic_title":"徳島大学総合科学部自然科学研究 = Natural Science Research, The University of Tokushima","bibliographic_titleLang":"ja"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"To begin the examination of the role of human type carbohydrate moiety of tissue plasminogen activator\n(t-PA) on the binding of the enzyme to fibrin，the naturally glycosylated enzyme was produced by microcarrier\nculture of human cells established from normal uterine muscle. The cells grown on microcarrers in Haunks' \nMEM supplemented with 10 % FBS (1.6 x 10 6 cells/ml) rapidly detached themselves from microcarrier in a\nserum-free medium (t-PA production medium) within 5 days, and it was difficult to produce t-PA for long time \n(t-PA production: an average of 3 IU/ml/day over 5 days). Addition of 0.5% beef extract to the serum-free\nmedium suppressed their detaching from microcarriers. By regulating the pH (7.4) and dissolved oxygen(4 \nppm) of the serum free medium，the cell density of microcarrier culture increased to 1.2 x10 7 cells/ml and t-PA\nwas produced over 38 days (t-PA production: an average of 836 IU/ml/day over 38 days). Native t-PAs purified\nto homogeneity from the culture broth had the molecular weight of 63,000 and 65,000 containing 5.6 and 8.5 %\ncarbohydrate，respectively (molecular mass of protein moiety was calculated to be 59,500). By enzymatic\ndigestion of carbohydrate moiety in native t-PA，we obtained partially deglycosylated t-PA with molecular\nweights of 60,000 and 62,000. Completely deglycosylated t-PA was obtained by t-PA production in the\npresence of 10μg/ml tunicamycin (N-glycosylation inhibitor). This suggests that N-glycosylationof t-PA\noccurs while O-glycosylation is absent. The binding strength of these enzymes to fibrin increased with\ndecrease of the carbohydrate content. The carbohydrate moiety of human type glycosylated t-PA probably\nmodulates the binding strength of the enzyme to fibrin.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"徳島大学総合科学部","subitem_publisher_language":"ja"}]},"item_10001_source_id_9":{"attribute_name":"収録物ID","attribute_value_mlt":[{"subitem_source_identifier":"09146385","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"AN10065859","subitem_source_identifier_type":"NCID"}]},"item_10001_version_type_20":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_1715043197608":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access"}]},"item_1723180141928":{"attribute_name":"item_1723180141928","attribute_value_mlt":[{"subitem_identifier_type":"URI","subitem_identifier_uri":"185295"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"三木, 瑞枝","creatorNameLang":"ja"},{"creatorName":"ミキ, ミズエ","creatorNameLang":"ja-Kana"},{"creatorName":"Miki, Mizue","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"谷本, 久美","creatorNameLang":"ja"},{"creatorName":"タニモト, クミ","creatorNameLang":"ja-Kana"},{"creatorName":"Tanimoto, Kumi","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"四方, 知子","creatorNameLang":"ja"},{"creatorName":"シカタ, トモコ","creatorNameLang":"ja-Kana"},{"creatorName":"Shikata, Tomoko","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"横井川, 久己男","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"1242","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2011-08-11"}],"displaytype":"detail","filename":"EID185295.pdf","filesize":[{"value":"880 KB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://tokushima-u.repo.nii.ac.jp/record/2001118/files/EID185295.pdf"},"version_id":"08653497-e2f9-4a2e-a43d-4a638e4adb1a"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Tissue plasminogen activator","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"cell culture","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"carbohydrate","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"animal cell","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"fibrinolysis","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"item_resource_type","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Production of Human Type Glycosylated Tissue Plasminogen Activator and the Role of Its Carbohydrate Moiety","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Production of Human Type Glycosylated Tissue Plasminogen Activator and the Role of Its Carbohydrate Moiety","subitem_title_language":"en"}]},"item_type_id":"40001","owner":"7","path":["1713853297799","1716344317782"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2011-08-11"},"publish_date":"2011-08-11","publish_status":"0","recid":"2001118","relation_version_is_last":true,"title":["Production of Human Type Glycosylated Tissue Plasminogen Activator and the Role of Its Carbohydrate Moiety"],"weko_creator_id":"7","weko_shared_id":-1},"updated":"2024-10-09T10:31:42.720042+00:00"}