{"created":"2024-10-25T07:11:02.290002+00:00","id":2002443,"links":{},"metadata":{"_buckets":{"deposit":"5dc4e24d-0949-4ffa-a3be-6af1bacb156a"},"_deposit":{"created_by":7,"id":"2002443","owners":[7],"pid":{"revision_id":0,"type":"depid","value":"2002443"},"status":"published"},"_oai":{"id":"oai:tokushima-u.repo.nii.ac.jp:02002443","sets":["1713853213384:1713853296295:1716267877313:1716268556148"]},"author_link":["1271","1687","484","1674","1651","1691","1653"],"item_10001_alternative_title_1":{"attribute_name":"タイトル別表記","attribute_value_mlt":[{"subitem_alternative_title":"Streptococcus intermedius バイオフィルム形成に及ぼす細胞外DNA及びDNA結合タンパク質の影響","subitem_alternative_title_language":"ja"},{"subitem_alternative_title":"Effect of eDNA on biofilm formation","subitem_alternative_title_language":"en"}]},"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2013-03-29","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"270","bibliographicPageStart":"260","bibliographicVolumeNumber":"115","bibliographic_titles":[{"bibliographic_title":"Journal of Applied Microbiology","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Aims: The aim of this study was to clarify the effects of homologous and heterologous extracellular DNAs (eDNAs) and histone-like DNA binding protein (HLP) on Streptococcus intermedius biofilm development and rigidity.\nMethods and Results: Formed biofilm mass was measured with 0.1% crystal violet staining method and observed with a scanning electron microscope. The localizations of eDNA and extracellular HLP (eHLP) in formed biofilm were detected by staining with 7-hydoxyl-9H-(1,3-dichloro-9,9-dimethylacridin-2-one) and anti-HLP antibody without fixation, respectively. DNase I treatment (200 U ml-1) markedly decreased biofilm formation and cell density in biofilms. Co-localization of eHLP and eDNA in biofilm was confirmed. The addition of eDNA (up to 1 μg ml-1) purified from S. intermedius, other Gram-positive, –negative bacteria, or human KB cells into the S. intermedius culture increased the biofilm mass of all tested strains of S. intermedius, wild-type, HLP down-regulated strain, and control strains. In contrast, the addition of eDNA (> 1 μg ml-1) decreased the biofilm mass of all S. intermedius strains. \nConclusions: These findings demonstrated that eDNA and eHLP play crucial roles in biofilm development and its rigidity.\nSignificance and Impact of the Study: eDNA- and HLP-targeting strategies may be applicable to novel treatments for bacterial biofilm-related infectious diseases.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"The Society for Applied Microbiology","subitem_publisher_language":"en"},{"subitem_publisher":"John Wiley & Sons Inc","subitem_publisher_language":"en"}]},"item_10001_rights_15":{"attribute_name":"権利情報","attribute_value_mlt":[{"subitem_rights":"This is the peer reviewed version of the following article: Nur, A., Hirota, K., Yumoto, H., Hirao, K., Liu, D., Takahashi, K., Murakami, K., Matsuo, T., Shu, R. and Miyake, Y. (2013), Effects of extracellular DNA and DNA‐binding protein on the development of a Streptococcus intermedius biofilm. J Appl Microbiol, 115: 260-270., which has been published in final form at https://doi.org/10.1111/jam.12202. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.","subitem_rights_language":"en"}]},"item_10001_source_id_9":{"attribute_name":"収録物ID","attribute_value_mlt":[{"subitem_source_identifier":"13652672","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"13645072","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"AA11115564","subitem_source_identifier_type":"NCID"}]},"item_10001_version_type_20":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_be7fb7dd8ff6fe43","subitem_version_type":"NA"}]},"item_1714461018643":{"attribute_name":"報告番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第2757号"}]},"item_1714461102074":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（歯学）","subitem_degreename_language":"ja"}]},"item_1714461118377":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2014-09-10"}]},"item_1714461137393":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_language":"ja","subitem_degreegrantor_name":"徳島大学"}]}]},"item_1715043197608":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access"}]},"item_1718868208704":{"attribute_name":"備考","attribute_value_mlt":[{"subitem_textarea_language":"ja","subitem_textarea_value":"内容要旨・審査要旨・論文本文の公開:\n内容要旨 : LID201409191007.pdf\n審査要旨 : LID201409191008.pdf\n論文本文 : k2757_fulltext.pdf\n本論文は，著者Asikin Nurの学位論文として提出され，学位審査・授与の対象となっている。\n著者の申請により要約(2014-09-20公開)に替えて論文全文を公開(2019-10-09)\n学位授与者所属 : 徳島大学大学院口腔科学教育部（口腔科学専攻）"}]},"item_1718868303842":{"attribute_name":"学位記番号","attribute_value_mlt":[{"subitem_text_language":"ja","subitem_text_value":"甲口第386号"}]},"item_1722929371688":{"attribute_name":"出版社版DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_language":"ja","subitem_relation_name_text":"10.1111/jam.12202"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1111/jam.12202","subitem_relation_type_select":"DOI"}}]},"item_1723180141928":{"attribute_name":"EID","attribute_value_mlt":[{"subitem_identifier_type":"URI","subitem_identifier_uri":"264649"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"アシキン, ヌル","creatorNameLang":"ja"},{"creatorName":"アシキン, ヌル","creatorNameLang":"ja-Kana"},{"creatorName":"Asikin, 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development","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Streptococcus intermedius","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"DNA concentration","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Effects of extracellular DNA and DNA-binding protein on the development of a Streptococcus intermedius biofilm","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Effects of extracellular DNA and DNA-binding protein on the development of a Streptococcus intermedius 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