{"created":"2024-10-25T07:26:08.121471+00:00","id":2002629,"links":{},"metadata":{"_buckets":{"deposit":"ad721783-3c8d-4126-959b-4a1b8d0334cf"},"_deposit":{"created_by":7,"id":"2002629","owners":[7],"pid":{"revision_id":0,"type":"depid","value":"2002629"},"status":"published"},"_oai":{"id":"oai:tokushima-u.repo.nii.ac.jp:02002629","sets":["1713853213384:1713853296295:1716267876683:1716268551628"]},"author_link":["294","1154","1259","517","791","1253"],"item_10001_alternative_title_1":{"attribute_name":"タイトル別表記","attribute_value_mlt":[{"subitem_alternative_title":"Cyclopamineは大腸癌癌幹細胞のソニック・ヘッジホッグとその下流遺伝子発現を抑制する","subitem_alternative_title_language":"ja"},{"subitem_alternative_title":"Sonic Hedgehog Downstream Genes in Cancer Stem Cells","subitem_alternative_title_language":"en"}]},"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2014-11-03","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"11","bibliographicPageEnd":"6344","bibliographicPageStart":"6339","bibliographicVolumeNumber":"34","bibliographic_titles":[{"bibliographic_title":"Anticancer Research","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Backround: Most solid cancers including colon cancer are believed to be initiated from and maintained by cancer stem cells (CSCs), that are responsible for treatment resistance, resulting in tumor relapse. The aim of this study was to clarify the possible role of the Sonic Hedgehog (Shh) signaling pathway in the regulation of cancer stem cells. Materials and Methods: The HCT-116 cell line was cultured with fetal bovine serum in RPMI-1640 medium and its sphere was grown in serum-free non-adherent culture. Gene expressions were analyzed by quantitative real-time polymerase chain reaction (qRT-PCR) from cells treated with and without cyclopamine. Results: HCT-116 sphere-derived cells grown in serum-free, non-adherent culture, showed significantly increased expression of stem cell markers, Shh downstream genes and epithelial-mesenchymal transition (EMT) markers compared to parental cells grown in conventional culture. The expression of stemness markers, Shh downstream genes and EMT markers were higher in cancer spheres than the parental cell line and down-regulated by cyclopamine treatment in a dose-dependent manner. Conclusion: Overall, these findings show that cyclopamine treatment could down-regulate the expression of stemness markers, shh downstream genes and EMT markers on HCT-116 spheres.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"The International Institute of Anticancer Research","subitem_publisher_language":"en"}]},"item_10001_source_id_9":{"attribute_name":"収録物ID","attribute_value_mlt":[{"subitem_source_identifier":"02507005","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"17917530","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"AA10625860","subitem_source_identifier_type":"NCID"},{"subitem_source_identifier":"AA12440673","subitem_source_identifier_type":"NCID"}]},"item_10001_version_type_20":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_be7fb7dd8ff6fe43","subitem_version_type":"NA"}]},"item_1714461018643":{"attribute_name":"報告番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第2796号"}]},"item_1714461102074":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士(医学)","subitem_degreename_language":"ja"}]},"item_1714461118377":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2015-03-23"}]},"item_1714461137393":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_language":"ja","subitem_degreegrantor_name":"徳島大学"}]}]},"item_1715043197608":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access"}]},"item_1718868208704":{"attribute_name":"備考","attribute_value_mlt":[{"subitem_textarea_language":"ja","subitem_textarea_value":"内容要旨・審査要旨・論文本文の公開:\n内容要旨・審査要旨 : LID201505251001.pdf\n論文本文 : k2796_fulltext.pdf\n本論文は,著者BAT-ERDENE BATSAIKHANの学位論文として提出され, 学位審査・授与の対象となっている。\n著者の申請により要約(2015-05-25公開)に替えて論文全文を公開(2022-12-14)\n学位授与者所属 : 徳島大学大学院医科学教育部(医学専攻)"}]},"item_1718868303842":{"attribute_name":"学位記番号","attribute_value_mlt":[{"subitem_text_language":"ja","subitem_text_value":"甲医第1238号"}]},"item_1722929371688":{"attribute_name":"出版社版DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://ar.iiarjournals.org/content/anticanres/34/11/6339.full.pdf","subitem_relation_type_select":"URI"}}]},"item_1723180141928":{"attribute_name":"EID","attribute_value_mlt":[{"subitem_identifier_type":"URI","subitem_identifier_uri":"297570"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"バトサイハン, バトエルデネ","creatorNameLang":"ja"},{"creatorName":"バトサイハン, バトエルデネ","creatorNameLang":"ja-Kana"},{"creatorName":"Batsaikhan, Bat-Erdene","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"吉川, 幸造","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"294","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"栗田, 信浩","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"1154","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"岩田, 貴","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"1259","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"髙須, 千絵","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"517","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"柏原, 秀也","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"791","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"島田, 光生","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"1253","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2015-05-25"}],"displaytype":"detail","filename":"LID201505251001.pdf","filesize":[{"value":"134 KB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"objectType":"abstract","url":"https://tokushima-u.repo.nii.ac.jp/record/2002629/files/LID201505251001.pdf"},"version_id":"a12fe99c-2ecf-4dcb-9a74-d5485db7faaa"},{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2022-12-14"}],"displaytype":"detail","filename":"k2796_fulltext.pdf","filesize":[{"value":"229 KB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://tokushima-u.repo.nii.ac.jp/record/2002629/files/k2796_fulltext.pdf"},"version_id":"0e3b8258-61da-49a6-a1e1-59e20817d933"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Cancer stem-like cells","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"epithelial-mesenchymal transition","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"cancer cells","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"sonic hedgehog","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Cyclopamine Decreased the Expression of Sonic Hedgehog and its Downstream Genes in Colon Cancer Stem Cells","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Cyclopamine Decreased the Expression of Sonic Hedgehog and its Downstream Genes in Colon Cancer Stem Cells","subitem_title_language":"en"}]},"item_type_id":"40001","owner":"7","path":["1716268551628"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2015-05-25"},"publish_date":"2015-05-25","publish_status":"0","recid":"2002629","relation_version_is_last":true,"title":["Cyclopamine Decreased the Expression of Sonic Hedgehog and its Downstream Genes in Colon Cancer Stem Cells"],"weko_creator_id":"7","weko_shared_id":-1},"updated":"2024-10-25T07:26:16.323606+00:00"}