{"created":"2024-10-25T07:33:48.140987+00:00","id":2002734,"links":{},"metadata":{"_buckets":{"deposit":"538ddcfd-af97-4686-8b9c-3d17c374504f"},"_deposit":{"created_by":7,"id":"2002734","owners":[7],"pid":{"revision_id":0,"type":"depid","value":"2002734"},"status":"published"},"_oai":{"id":"oai:tokushima-u.repo.nii.ac.jp:02002734","sets":["1713853213384:1713853296295:1716267877313:1716268556148"]},"author_link":["835","1255","1264","570","1260","1709","1652","1648"],"item_10001_alternative_title_1":{"attribute_name":"タイトル別表記","attribute_value_mlt":[{"subitem_alternative_title":"MC3T3-E1細胞における圧縮応力誘導性miR494-3pによる細胞増殖の阻害","subitem_alternative_title_language":"ja"},{"subitem_alternative_title":"FUNCTION OF miR-494-3p IN OSTEOBLASTS","subitem_alternative_title_language":"en"}]},"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2015-03-17","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"4","bibliographicPageEnd":"462","bibliographicPageStart":"456","bibliographicVolumeNumber":"120","bibliographic_titles":[{"bibliographic_title":"Journal of Bioscience and Bioengineering","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Mechanical stimuli regulate fundamental cell processes such as proliferation, differentiation, and morphogenesis. We attempted to identify microRNA (miRNA) whose expression is changed during compressive treatment in MC3T3-E1, a pre-osteoblastic cell line. Microarray analysis followed by reverse transcription-quantitative polymerase chain reaction revealed that compressive force at 294 Pa for 24 h in MC3T3-E1 cells increased levels of miR-494-3p, miR-146a-5p, miR-210-3p, and miR-1247-3p. Among these miRNAs, miR-494-3p was found to inhibit cell proliferation in MC3T3-E1 cells. Furthermore, cells subjected to compressive force showed slower cell growth compared with control cells. Levels of mRNA for fibroblast growth factor receptor 2 (FGFR2) and Rho-associated coiled-coil kinase 1 (ROCK1), which were predicted to be targets of miR-494-3p, were decreased by compressive force or overexpression of miR-494-3p mimics in MC3T3-E1 cells. Furthermore, binding sites of miR-494-3p within 3'-untranslated regions of Fgfr2 and Rock1 were determined using luciferase reporter assay. In conclusion, compressive force affected expressions of several miRNAs including miR-494-3p in MC3T3-E1 cells. Compressive force might inhibit cell proliferation in osteoblasts by up-regulating miR-494-3p followed by FGFR2 and ROCK1 gene repressions.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"The Society for Biotechnology, Japan","subitem_publisher_language":"en"},{"subitem_publisher":"Elsevier","subitem_publisher_language":"en"}]},"item_10001_rights_15":{"attribute_name":"権利情報","attribute_value_mlt":[{"subitem_rights":"Copyright © 2015 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.\nThis manuscript version is made available under the CC-BY-NC-ND 4.0 license ( http://creativecommons.org/licenses/by-nc-nd/4.0/ )","subitem_rights_language":"en"}]},"item_10001_source_id_9":{"attribute_name":"収録物ID","attribute_value_mlt":[{"subitem_source_identifier":"13474421","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"13891723","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"AA11644703","subitem_source_identifier_type":"NCID"},{"subitem_source_identifier":"AA11307678","subitem_source_identifier_type":"NCID"}]},"item_10001_version_type_20":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_be7fb7dd8ff6fe43","subitem_version_type":"NA"}]},"item_1714461018643":{"attribute_name":"報告番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第2858号"}]},"item_1714461102074":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（歯学）","subitem_degreename_language":"ja"}]},"item_1714461118377":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2015-06-11"}]},"item_1714461137393":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_language":"ja","subitem_degreegrantor_name":"徳島大学"}]}]},"item_1715043197608":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access"}]},"item_1718868208704":{"attribute_name":"備考","attribute_value_mlt":[{"subitem_textarea_language":"ja","subitem_textarea_value":"内容要旨・審査要旨・論文本文の公開:\n内容要旨 : LID201507171004.pdf\n審査要旨 : LID201507171005.pdf\n論文本文 : k2858_fulltext.pdf\n本論文は，著者Yuki Iwawakiの学位論文として提出され，学位審査・授与の対象となっている。\n著者の申請により要約(2015-07-18公開)に替えて論文全文を公開(2019-08-22)\n学位授与者所属 : 徳島大学大学院口腔科学教育部（口腔科学専攻）"}]},"item_1718868303842":{"attribute_name":"学位記番号","attribute_value_mlt":[{"subitem_text_language":"ja","subitem_text_value":"甲口第397号"}]},"item_1722929371688":{"attribute_name":"出版社版DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_language":"ja","subitem_relation_name_text":"10.1016/j.jbiosc.2015.02.006"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1016/j.jbiosc.2015.02.006","subitem_relation_type_select":"DOI"}}]},"item_1723180141928":{"attribute_name":"EID","attribute_value_mlt":[{"subitem_identifier_type":"URI","subitem_identifier_uri":"316124"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"岩脇, 有軌","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"835","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"水澤, 典子","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"1255","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"岩田, 武男","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"1264","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"ヒガキ, ノブアキ","creatorNameLang":"ja"},{"creatorName":"ヒガキ, ノブアキ","creatorNameLang":"ja-Kana"},{"creatorName":"Higaki, Nobuaki","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"後藤, 崇晴","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"570","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"渡邉, 恵","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"1260","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"友竹, 偉則","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"1709","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"市川, 哲雄","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"1652","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"吉本, 勝彦","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"1648","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2015-07-18"}],"displaytype":"detail","filename":"LID201507171004.pdf","filesize":[{"value":"186 KB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"objectType":"abstract","url":"https://tokushima-u.repo.nii.ac.jp/record/2002734/files/LID201507171004.pdf"},"version_id":"ace75b09-0842-4d94-abb4-b7cdf2754d76"},{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2015-07-18"}],"displaytype":"detail","filename":"LID201507171005.pdf","filesize":[{"value":"490 KB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"objectType":"other","url":"https://tokushima-u.repo.nii.ac.jp/record/2002734/files/LID201507171005.pdf"},"version_id":"1df1f53c-3898-4139-80a8-43d90bbbce7c"},{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2019-08-22"}],"displaytype":"detail","filename":"k2858_fulltext.pdf","filesize":[{"value":"2.46 MB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://tokushima-u.repo.nii.ac.jp/record/2002734/files/k2858_fulltext.pdf"},"version_id":"8178feda-6868-403b-b71c-ff018e0e7fa2"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Mechanical stimuli","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Compressive force","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"MicroRNA","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Osteoblasts","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Cell proliferation","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"MiR-494-3p induced by compressive force inhibits cell proliferation in MC3T3-E1 cells","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"MiR-494-3p induced by compressive force inhibits cell proliferation in MC3T3-E1 cells","subitem_title_language":"en"}]},"item_type_id":"40001","owner":"7","path":["1716268556148"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2015-07-17"},"publish_date":"2015-07-17","publish_status":"0","recid":"2002734","relation_version_is_last":true,"title":["MiR-494-3p induced by compressive force inhibits cell proliferation in MC3T3-E1 cells"],"weko_creator_id":"7","weko_shared_id":-1},"updated":"2024-10-25T07:34:01.266852+00:00"}