{"created":"2024-10-25T07:35:04.830768+00:00","id":2002749,"links":{},"metadata":{"_buckets":{"deposit":"ce7949eb-8cdf-43b5-af60-9cfe0dc0a71b"},"_deposit":{"created_by":7,"id":"2002749","owners":[7],"pid":{"revision_id":0,"type":"depid","value":"2002749"},"status":"published"},"_oai":{"id":"oai:tokushima-u.repo.nii.ac.jp:02002749","sets":["1713853213384:1713853296295:1716267877313:1716268556148"]},"author_link":["418","323","316"],"control_number":"2002749","item_10001_alternative_title_1":{"attribute_name":"タイトル別表記","attribute_value_mlt":[{"subitem_alternative_title":"エストロゲン受容体陽性乳がん細胞において、BIG3は複数のKaryopherin-αを介したPHB2のエストロゲン依存性核移行を阻害する","subitem_alternative_title_language":"ja"}]},"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2015-06-08","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"6","bibliographicPageStart":"e0127707","bibliographicVolumeNumber":"10","bibliographic_titles":[{"bibliographic_title":"PLOS ONE","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"We recently reported that brefeldin A-inhibited guanine nucleotide-exchange protein 3 (BIG3) binds Prohibitin 2 (PHB2) in cytoplasm, thereby causing a loss of function of the PHB2 tumor suppressor in the nuclei of breast cancer cells. However, little is known regarding the mechanism by which BIG3 inhibits the nuclear translocation of PHB2 into breast cancer cells. Here, we report that BIG3 blocks the estrogen (E2)-dependent nuclear import of PHB2 via the karyopherin alpha (KPNA) family in breast cancer cells. We found that overexpressed PHB2 interacted with KPNA1, KPNA5, and KPNA6, thereby leading to the E2-dependent translocation of PHB2 into the nuclei of breast cancer cells. More importantly, knockdown of each endogenous KPNA by siRNA caused a significant inhibition of E2-dependent translocation of PHB2 in BIG3-depleted breast cancer cells, thereby enhancing activation of estrogen receptor alpha (ERα). These data indicated that BIG3 may block the KPNAs (KPNA1, KPNA5, and KPNA6) binding region(s) of PHB2, thereby leading to inhibition of KPNAs-mediated PHB2 nuclear translocation in the presence of E2 in breast cancer cells. Understanding this regulation of PHB2 nuclear import may provide therapeutic strategies for controlling E2/ERα signals in breast cancer cells.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"PLOS","subitem_publisher_language":"en"}]},"item_10001_rights_15":{"attribute_name":"権利情報","attribute_value_mlt":[{"subitem_rights":"Copyright: © 2015 Kim et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.","subitem_rights_language":"en"}]},"item_10001_source_id_9":{"attribute_name":"収録物ID","attribute_value_mlt":[{"subitem_source_identifier":"19326203","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_be7fb7dd8ff6fe43","subitem_version_type":"NA"}]},"item_1714461018643":{"attribute_name":"報告番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第2860号"}]},"item_1714461102074":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（歯学）","subitem_degreename_language":"ja"}]},"item_1714461118377":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2015-07-16"}]},"item_1714461137393":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_language":"ja","subitem_degreegrantor_name":"徳島大学"}]}]},"item_1715043197608":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access"}]},"item_1718868208704":{"attribute_name":"備考","attribute_value_mlt":[{"subitem_textarea_language":"ja","subitem_textarea_value":"内容要旨・審査要旨・論文本文の公開:\n内容要旨 : LID201507301001.pdf\n審査要旨 : LID201507301002.pdf\n論文本文 : LID201507301003.pdf\n本論文は, 著者Nam-Hee Kimの学位論文として提出され, 学位審査・授与の対象となっている。\n学位授与者所属 : 徳島大学大学院口腔科学教育部（口腔科学専攻）"}]},"item_1718868303842":{"attribute_name":"学位記番号","attribute_value_mlt":[{"subitem_text_language":"ja","subitem_text_value":"甲口第399号"}]},"item_1722929371688":{"attribute_name":"出版社版DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_language":"ja","subitem_relation_name_text":"10.1371/journal.pone.0127707"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1371/journal.pone.0127707","subitem_relation_type_select":"DOI"}}]},"item_1723180141928":{"attribute_name":"EID","attribute_value_mlt":[{"subitem_identifier_type":"URI","subitem_identifier_uri":"295242"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"金, 南希","creatorNameLang":"ja"},{"creatorName":"キム, ナムヒ","creatorNameLang":"ja-Kana"},{"creatorName":"Kim, Nam-Hee","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"吉丸, 哲郎","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"418","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Chen, Yi-An","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"松尾, 泰佑","creatorNameLang":"ja"},{"creatorName":"マツオ, タイスケ","creatorNameLang":"ja-Kana"},{"creatorName":"Matsuo, Taisuke","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"小松, 正人","creatorNameLang":"ja"},{"creatorName":"コマツ, マサト","creatorNameLang":"ja-Kana"},{"creatorName":"Komatsu, Masato","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"ミヨシ, ヤスオ","creatorNameLang":"ja"},{"creatorName":"ミヨシ, ヤスオ","creatorNameLang":"ja-Kana"},{"creatorName":"Miyoshi, Yasuo","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"田中, 栄二","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"323","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"ササ, ミツノリ","creatorNameLang":"ja"},{"creatorName":"ササ, ミツノリ","creatorNameLang":"ja-Kana"},{"creatorName":"Sasa, Mitsunori","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"ミズグチ, ケンジ","creatorNameLang":"ja"},{"creatorName":"ミズグチ, ケンジ","creatorNameLang":"ja-Kana"},{"creatorName":"Mizuguchi, Kenji","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"片桐, 豊雅","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"316","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2015-07-30"}],"displaytype":"detail","filename":"LID201507301001.pdf","filesize":[{"value":"75.7 KB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"objectType":"abstract","url":"https://tokushima-u.repo.nii.ac.jp/record/2002749/files/LID201507301001.pdf"},"version_id":"7c9c34cf-ffe2-4928-933f-cb6fc609d5e9"},{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2015-07-30"}],"displaytype":"detail","filename":"LID201507301002.pdf","filesize":[{"value":"299 KB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"objectType":"other","url":"https://tokushima-u.repo.nii.ac.jp/record/2002749/files/LID201507301002.pdf"},"version_id":"b4f1514f-7c12-43a2-b907-fab6a507eaff"},{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2015-07-30"}],"displaytype":"detail","filename":"LID201507301003.pdf","filesize":[{"value":"2.91 MB"}],"format":"application/pdf","licensetype":"license_0","mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://tokushima-u.repo.nii.ac.jp/record/2002749/files/LID201507301003.pdf"},"version_id":"8189db39-0b38-4d17-a018-eaada44e8da7"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Karyopherin-alpha","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"estrogen","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"nuclear translocation","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"breast cancer","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"BIG3","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"PHB2","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"BIG3 Inhibits the Estrogen-Dependent Nuclear Translocation of PHB2 via Multiple Karyopherin-Alpha Proteins in Breast Cancer Cells","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"BIG3 Inhibits the Estrogen-Dependent Nuclear Translocation of PHB2 via Multiple Karyopherin-Alpha Proteins in Breast Cancer Cells","subitem_title_language":"en"}]},"item_type_id":"40001","owner":"7","path":["1716268556148"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2015-10-23"},"publish_date":"2015-10-23","publish_status":"0","recid":"2002749","relation_version_is_last":true,"title":["BIG3 Inhibits the Estrogen-Dependent Nuclear Translocation of PHB2 via Multiple Karyopherin-Alpha Proteins in Breast Cancer Cells"],"weko_creator_id":"7","weko_shared_id":-1},"updated":"2024-11-22T01:36:20.770736+00:00"}