{"created":"2024-10-25T07:38:27.587487+00:00","id":2002788,"links":{},"metadata":{"_buckets":{"deposit":"6da1218b-9dd1-4a38-bfc5-7756ac61e70a"},"_deposit":{"created_by":7,"id":"2002788","owners":[7],"pid":{"revision_id":0,"type":"depid","value":"2002788"},"status":"published"},"_oai":{"id":"oai:tokushima-u.repo.nii.ac.jp:02002788","sets":["1713853213384:1713853295607"]},"author_link":["508","511","1679","1658"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2014-02","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1-2","bibliographicPageEnd":"150","bibliographicPageStart":"137","bibliographicVolumeNumber":"61","bibliographic_titles":[{"bibliographic_title":"The journal of medical investigation : JMI","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Adenylate kinase isozyme 2 (AK2) is located in mitochondrial intermembrane\nspace and regulates energy metabolism by reversibly converting ATP and AMP to 2 ADPs.\nWe previously demonstrated that disruption of the Drosophila melanogaster AK2 gene\n(Dak2) resulted in growth arrest during the larval stage and subsequent death. Two other\ngroups found that human AK2 mutations cause reticular dysgenesis, a form of severe combined\nimmunodeficiency (SCID) that is associated with severe hematopoietic defects and\nsensorineural deafness. However, the mechanisms underlying differential outcomes of AK2\ndeficiency in Drosophila and human systems remain unknown. In this study, effects of\ntissue-specific inactivation of the Dak2 gene on Drosophila development were analyzed\nusing RNAi-mediated gene knockdown. In addition, to investigate the roles of AK2 in the\nregulation of gene expression during development, microarray analysis was performed using\nRNA from first and second instar larvae of Dak2-deficient mutant and wild-type D.\nmelanogaster. Knockdown of Dak2 in all germ layers caused cessation of growth and subsequent\ndeath of flies. Microarray analysis revealed that Dak2 deficiency downregulates\nvarious genes, particularly those involved in the proteasomal function and in mitochondrial\ntranslation machinery. These data indicate that adenine nucleotide interconversion by\nDak2 is crucial for developmental processes of Drosophila melanogaster.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_source_id_9":{"attribute_name":"収録物ID","attribute_value_mlt":[{"subitem_source_identifier":"13431420","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"AA11166929","subitem_source_identifier_type":"NCID"}]},"item_10001_version_type_20":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_1715043197608":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access"}]},"item_1723180141928":{"attribute_name":"EID","attribute_value_mlt":[{"subitem_identifier_type":"URI","subitem_identifier_uri":"277119"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"堀口, 大吾","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"508","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"フカ, ミユキ","creatorNameLang":"ja"},{"creatorName":"フカ, ミユキ","creatorNameLang":"ja-Kana"},{"creatorName":"Fuka, Miyuki","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"フジサワ, コウイチ","creatorNameLang":"ja"},{"creatorName":"フジサワ, コウイチ","creatorNameLang":"ja-Kana"},{"creatorName":"Fujisawa, Koichi","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"谷村, 綾子","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"511","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"三好, 圭子","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"1679","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"ムラカミ, リュウタロウ","creatorNameLang":"ja"},{"creatorName":"ムラカミ, リュウタロウ","creatorNameLang":"ja-Kana"},{"creatorName":"Murakami, Ryutaro","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"野間, 隆文","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"1658","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2016-01-27"}],"displaytype":"detail","filename":"LID201601275001.pdf","filesize":[{"value":"11.7 MB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://tokushima-u.repo.nii.ac.jp/record/2002788/files/LID201601275001.pdf"},"version_id":"5718a117-c84b-4c17-8fca-430fb861ee56"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Dak2","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"gene expression","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"in silico analysis","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"microarray","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"mitochondria","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Adenylate kinase 2 deficiency limits survival and regulates various genes during larval stages of Drosophila melanogaster","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Adenylate kinase 2 deficiency limits survival and regulates various genes during larval stages of Drosophila melanogaster","subitem_title_language":"en"}]},"item_type_id":"40001","owner":"7","path":["1713853295607"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2016-01-27"},"publish_date":"2016-01-27","publish_status":"0","recid":"2002788","relation_version_is_last":true,"title":["Adenylate kinase 2 deficiency limits survival and regulates various genes during larval stages of Drosophila melanogaster"],"weko_creator_id":"7","weko_shared_id":-1},"updated":"2024-10-28T01:35:18.522404+00:00"}