{"created":"2024-10-25T07:41:51.540579+00:00","id":2002832,"links":{},"metadata":{"_buckets":{"deposit":"19f687b3-d9b6-4d6f-bfd1-2800d53ccefb"},"_deposit":{"created_by":7,"id":"2002832","owners":[7],"pid":{"revision_id":0,"type":"depid","value":"2002832"},"status":"published"},"_oai":{"id":"oai:tokushima-u.repo.nii.ac.jp:02002832","sets":["1713829974766:1713854681789:1716364516170:1716365539922","1713853213384:1713853295607"]},"author_link":["1594","921","246"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2014-08","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3-4","bibliographicPageEnd":"379","bibliographicPageStart":"374","bibliographicVolumeNumber":"61","bibliographic_titles":[{"bibliographic_title":"The journal of medical investigation : JMI","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"We have previously generated a macaque-tropic human immunodeficiency virus type 1 (HIV-1mt) clone designated MN4/LSDQgtu by genetic manipulation from a parental virus that replicates poorly in rhesus macaque cells. In rhesus cell line M1.3S and peripheral blood mononuclear cells (PBMCs), MN4/LSDQgtu grows comparably to a standard simian immunodeficiency virus clone derived from the rhesus macaque (SIVmac239) that can induce the acquired immunodeficiency syndrome (AIDS) in the animals. In this study, we further modified the Vpr-coding region of MN4/LSDQgtu genome by introducing vpr gene of an SIV clone from the greater spot-nosed monkey (SIVgsn166) or vpx gene of SIVmac239 to generate four new clones for determining functional importance of the central genomic area. Furthermore, two clones with an additional Gag-p6 mutation were made to ensure the virion-packaging of Vpx. In addition, accessory gene mutant clones of MN4/LSDQgtu with a frame-shift mutation, including a vpr mutant, were constructed and their growth properties were examined. Infection experiments showed that newly constructed viruses all grew poorly to various degrees in M1.3S cells, relative to MN4/LSDQgtu. Together with the previous data, our results here show that vpr/vpx gene in the appropriate context of HIV-1 genome is critical for viral growth ability.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_source_id_9":{"attribute_name":"収録物ID","attribute_value_mlt":[{"subitem_source_identifier":"13431420","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"AA11166929","subitem_source_identifier_type":"NCID"}]},"item_10001_version_type_20":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_1715043197608":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open 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vpr/vpx genes derived from simian immunodeficiency viruses in place of their vpr regions","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Growth properties of macaque-tropic HIV-1 clones carrying vpr/vpx genes derived from simian immunodeficiency viruses in place of their vpr regions","subitem_title_language":"en"}]},"item_type_id":"40001","owner":"7","path":["1713853295607","1716365539922"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2016-05-26"},"publish_date":"2016-05-26","publish_status":"0","recid":"2002832","relation_version_is_last":true,"title":["Growth properties of macaque-tropic HIV-1 clones carrying vpr/vpx genes derived from simian immunodeficiency viruses in place of their vpr regions"],"weko_creator_id":"7","weko_shared_id":-1},"updated":"2025-01-29T05:44:21.204073+00:00"}