{"created":"2024-10-25T10:14:12.090238+00:00","id":2003354,"links":{},"metadata":{"_buckets":{"deposit":"826ac65c-cef5-4e4d-b10b-bf412be2c006"},"_deposit":{"created_by":7,"id":"2003354","owners":[7],"pid":{"revision_id":0,"type":"depid","value":"2003354"},"status":"published"},"_oai":{"id":"oai:tokushima-u.repo.nii.ac.jp:02003354","sets":["1713853213384:1713853295607"]},"author_link":["748","664"],"control_number":"2003354","item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2016-08-17","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"31481","bibliographicVolumeNumber":"6","bibliographic_titles":[{"bibliographic_title":"Scientific Reports","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Genome editing is a powerful technique for genome modification in molecular research and crop breeding, and has the great advantage of imparting novel desired traits to genetic resources. However, the genome editing of fruit tree plantlets remains to be established. In this study, we describe induction of a targeted gene mutation in the endogenous apple phytoene desaturase (PDS) gene using the CRISPR/Cas9 system. Four guide RNAs (gRNAs) were designed and stably transformed with Cas9 separately in apple. Clear and partial albino phenotypes were observed in 31.8% of regenerated plantlets for one gRNA, and bi-allelic mutations in apple PDS were confirmed by DNA sequencing. In addition, an 18-bp gRNA also induced a targeted mutation. These CRIPSR/Cas9 induced-mutations in the apple genome suggest activation of the NHEJ pathway, but with some involvement also of the HR pathway. Our results demonstrate that genome editing can be practically applied to modify the apple genome.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_rights_15":{"attribute_name":"権利情報","attribute_value_mlt":[{"subitem_rights":"Copyright ©2016 Authors.This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/","subitem_rights_language":"en"}]},"item_10001_source_id_9":{"attribute_name":"収録物ID","attribute_value_mlt":[{"subitem_source_identifier":"20452322","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_1715043197608":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access"}]},"item_1722929371688":{"attribute_name":"出版社版DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1038/srep31481","subitem_relation_type_select":"DOI"}}]},"item_1723180141928":{"attribute_name":"EID","attribute_value_mlt":[{"subitem_identifier_type":"URI","subitem_identifier_uri":"318870"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"ニシタニ, チカコ","creatorNameLang":"ja"},{"creatorName":"ニシタニ, チカコ","creatorNameLang":"ja-Kana"},{"creatorName":"Nishitani, Chikako","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"ヒライ, ナルミ","creatorNameLang":"ja"},{"creatorName":"ヒライ, ナルミ","creatorNameLang":"ja-Kana"},{"creatorName":"Hirai, Narumi","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"コモリ, サダオ","creatorNameLang":"ja"},{"creatorName":"コモリ, サダオ","creatorNameLang":"ja-Kana"},{"creatorName":"Komori, Sadao","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"ワダ, マサト","creatorNameLang":"ja"},{"creatorName":"ワダ, マサト","creatorNameLang":"ja-Kana"},{"creatorName":"Wada, Masato","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"オカダ, カズマ","creatorNameLang":"ja"},{"creatorName":"オカダ, カズマ","creatorNameLang":"ja-Kana"},{"creatorName":"Okada, Kazuma","creatorNameLang":"en"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"刑部, 敬史","creatorNameLang":"ja"},{"creatorName":"オサカベ, ケイシ","creatorNameLang":"ja-Kana"},{"creatorName":"Osakabe, Keishi","creatorNameLang":"en"}],"familyNames":[{"familyName":"刑部","familyNameLang":"ja"},{"familyName":"オサカベ","familyNameLang":"ja-Kana"},{"familyName":"Osakabe","familyNameLang":"en"}],"givenNames":[{"givenName":"敬史","givenNameLang":"ja"},{"givenName":"ケイシ","givenNameLang":"ja-Kana"},{"givenName":"Keishi","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"664","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"272329/profile-ja.html","nameIdentifierScheme":"徳島大学 教育研究者総覧","nameIdentifierURI":"http://pub2.db.tokushima-u.ac.jp/ERD/person/272329/profile-ja.html"},{"nameIdentifier":"70450335","nameIdentifierScheme":"e-Rad_Researcher","nameIdentifierURI":"https://nrid.nii.ac.jp/ja/search/?qm=70450335"}]},{"creatorNames":[{"creatorName":"ヤマモト, トシヤ","creatorNameLang":"ja"},{"creatorName":"ヤマモト, トシヤ","creatorNameLang":"ja-Kana"},{"creatorName":"Yamamoto, Toshiya","creatorNameLang":"en"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"刑部, 祐里子","creatorNameLang":"ja"},{"creatorName":"オサカベ, ユリコ","creatorNameLang":"ja-Kana"},{"creatorName":"Osakabe, Yuriko","creatorNameLang":"en"}],"familyNames":[{"familyName":"刑部","familyNameLang":"ja"},{"familyName":"オサカベ","familyNameLang":"ja-Kana"},{"familyName":"Osakabe","familyNameLang":"en"}],"givenNames":[{"givenName":"祐里子","givenNameLang":"ja"},{"givenName":"ユリコ","givenNameLang":"ja-Kana"},{"givenName":"Yuriko","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"748","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"50444071","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://nrid.nii.ac.jp/ja/search/?qm=50444071"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2017-06-01"}],"displaytype":"detail","filename":"LID201706014002.pdf","filesize":[{"value":"1.59 MB"}],"format":"application/pdf","licensetype":"license_0","mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://tokushima-u.repo.nii.ac.jp/record/2003354/files/LID201706014002.pdf"},"version_id":"c0eab203-44e7-4cbd-90bf-0acd40582490"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Efficient Genome Editing in Apple Using a CRISPR/Cas9 system","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Efficient Genome Editing in Apple Using a CRISPR/Cas9 system","subitem_title_language":"en"}]},"item_type_id":"40001","owner":"7","path":["1713853295607"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2017-06-05"},"publish_date":"2017-06-05","publish_status":"0","recid":"2003354","relation_version_is_last":true,"title":["Efficient Genome Editing in Apple Using a CRISPR/Cas9 system"],"weko_creator_id":"7","weko_shared_id":-1},"updated":"2025-02-13T06:36:08.340781+00:00"}