{"created":"2024-10-25T10:15:44.863070+00:00","id":2003372,"links":{},"metadata":{"_buckets":{"deposit":"2990ad42-1c07-41fa-916e-9c5ee098c87e"},"_deposit":{"created_by":7,"id":"2003372","owners":[7],"pid":{"revision_id":0,"type":"depid","value":"2003372"},"status":"published"},"_oai":{"id":"oai:tokushima-u.repo.nii.ac.jp:02003372","sets":["1713853213384:1713853296295:1716267876683:1716268551628"]},"author_link":["633","814","882","709","349","677","175","1555","1572","460","287"],"item_10001_alternative_title_1":{"attribute_name":"タイトル別表記","attribute_value_mlt":[{"subitem_alternative_title":"肝細胞癌におけるDes-gamma-carboxy prothrombinの産生はpoly-(ADP-ribose) polymerase-1によりプロトロンビン遺伝子の転写が亢進することによっておこる","subitem_alternative_title_language":"ja"},{"subitem_alternative_title":"DCP Production Mechanism in HCC","subitem_alternative_title_language":"en"}]},"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2017-04-07","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3","bibliographicPageEnd":"251","bibliographicPageStart":"242","bibliographicVolumeNumber":"95","bibliographic_titles":[{"bibliographic_title":"Digestion","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Background and Aim: Although des-gamma-carboxy prothrombin (DCP) is a well-known tumor marker for hepatocellular carcinoma (HCC), the mechanism of DCP production is unclear. This study aimed to investigate the mechanism how DCP is produced in HCC cells. Methods: Levels of mRNA and DCP were analyzed by real-time polymerase chain reaction and electro-chemiluminescence immunoassay respectively. Secreted alkaline phosphatase (SEAP) expression vectors including deletion mutants of the prothrombin gene promoter were constructed for reporter gene assay. The transcription factors bound to DNA fragments were analyzed by mass spectrometry. An electrophoretic mobility shift assay (EMSA) was performed using a biotin end-labeled DNA. Results: The prothrombin mRNA levels in all 5 DCP producing cell lines were appreciably high. However, those in 2 DCP non-producing cell lines were below detectable levels. A SEAP vector with -2985 to +27 showed a very high transcription activity in DCP-producing Huh-1 cells. However, transcription abruptly decreased when the vector with -2955 to +27 was transfected, and then remained at the similar levels with larger deletion mutants, indicating the existence of a cis-element at -2985 to -2955 (31-bp). Mass spectrometry analysis identified the protein that bound to the 31-bp DNA as poly-(ADP-ribose) polymerase-1 (PARP-1). Knockdown of the PARP-1 gene by small interfering RNA in Huh-1 cells induced marked inhibition of prothrombin gene transcription. The EMSA clearly showed that PARP-1 specifically binds to the 31-bp DNA fragment in the prothrombin gene promoter. Conclusions: Our data suggest that PARP-1 activates prothrombin gene transcription and that the excessive prothrombin gene transcription induces DCP production in DCP-producing HCC cells.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":" Karger AG","subitem_publisher_language":"en"}]},"item_10001_rights_15":{"attribute_name":"権利情報","attribute_value_mlt":[{"subitem_rights":"© 2017 S. Karger AG, Basel","subitem_rights_language":"en"}]},"item_10001_source_id_9":{"attribute_name":"収録物ID","attribute_value_mlt":[{"subitem_source_identifier":"00122823","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"14219867","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"AA00628636","subitem_source_identifier_type":"NCID"},{"subitem_source_identifier":"AA12781324","subitem_source_identifier_type":"NCID"}]},"item_10001_version_type_20":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_be7fb7dd8ff6fe43","subitem_version_type":"NA"}]},"item_1714461018643":{"attribute_name":"報告番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第3095号"}]},"item_1714461102074":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（医学）","subitem_degreename_language":"ja"}]},"item_1714461118377":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2017-05-25"}]},"item_1714461137393":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_language":"ja","subitem_degreegrantor_name":"徳島大学"}]}]},"item_1715043197608":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access"}]},"item_1718868208704":{"attribute_name":"備考","attribute_value_mlt":[{"subitem_textarea_language":"ja","subitem_textarea_value":"内容要旨・審査要旨・論文本文の公開:\n内容要旨・審査要旨：LID201706272001.pdf\n論文本文 : k3095_fulltext.pdf\n著者の申請により要約(2017-06-27公開)に替えて論文全文を公開(2018-06-15)\n本論文は, 著者Tatsuya Taniguchiの学位論文として提出され, 学位審査・授与の対象となっている。\n学位授与者所属 : 徳島大学大学院医科学教育部（医学専攻）"}]},"item_1718868303842":{"attribute_name":"学位記番号","attribute_value_mlt":[{"subitem_text_language":"ja","subitem_text_value":"甲医第1339号"}]},"item_1722929371688":{"attribute_name":"出版社版DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_language":"ja","subitem_relation_name_text":"10.1159/000470837"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1159/000470837","subitem_relation_type_select":"DOI"}}]},"item_1723180141928":{"attribute_name":"EID","attribute_value_mlt":[{"subitem_identifier_type":"URI","subitem_identifier_uri":"323889"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"谷口, 達哉","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"633","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"岸, 和弘","creatorNameLang":"ja"},{"creatorName":"キシ, カズヒロ","creatorNameLang":"ja-Kana"},{"creatorName":"Kishi, Kazuhiro","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"中川, 忠彦","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"814","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"田中, 宏典","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"882","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"田中, 貴大","creatorNameLang":"ja"},{"creatorName":"タナカ, タカヒロ","creatorNameLang":"ja-Kana"},{"creatorName":"Tanaka, Takahiro","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"友成, 哲","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"709","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"岡本, 耕一","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"349","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"曽我部, 正弘","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"677","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"宮本, 弘志","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"175","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"岡久, 稔也","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"1555","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"六車, 直樹","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"1572","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"カジモト, マユミ","creatorNameLang":"ja"},{"creatorName":"カジモト, マユミ","creatorNameLang":"ja-Kana"},{"creatorName":"Kajimoto, Mayumi","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"佐川, 幾子","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"460","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"高山, 哲治","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"287","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2017-06-27"}],"displaytype":"detail","filename":"LID201706272001.pdf","filesize":[{"value":"302 KB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"objectType":"abstract","url":"https://tokushima-u.repo.nii.ac.jp/record/2003372/files/LID201706272001.pdf"},"version_id":"0a6a2185-d0f0-40ab-a687-8449d6913df0"},{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2018-06-15"}],"displaytype":"detail","filename":"k3095_fulltext.pdf","filesize":[{"value":"6.59 MB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://tokushima-u.repo.nii.ac.jp/record/2003372/files/k3095_fulltext.pdf"},"version_id":"0e5f7d3b-eae4-4718-a872-22e1cc04eed4"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"des-gamma-carboxy prothrombin","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"PARP-1","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"hepatocellular carcinoma","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"tumor marker","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Biomarkers","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Prothrombin","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Poly-(ADP-Ribose) Polymerase-1 Promotes Prothrombin Gene Transcription and Produces Des-Gamma-Carboxy Prothrombin in Hepatocellular Carcinoma","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Poly-(ADP-Ribose) Polymerase-1 Promotes Prothrombin Gene Transcription and Produces Des-Gamma-Carboxy Prothrombin in Hepatocellular Carcinoma","subitem_title_language":"en"}]},"item_type_id":"40001","owner":"7","path":["1716268551628"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2017-06-27"},"publish_date":"2017-06-27","publish_status":"0","recid":"2003372","relation_version_is_last":true,"title":["Poly-(ADP-Ribose) Polymerase-1 Promotes Prothrombin Gene Transcription and Produces Des-Gamma-Carboxy Prothrombin in Hepatocellular Carcinoma"],"weko_creator_id":"7","weko_shared_id":-1},"updated":"2024-10-25T10:15:53.332002+00:00"}