{"created":"2024-10-28T02:24:10.552603+00:00","id":2003781,"links":{},"metadata":{"_buckets":{"deposit":"7f0f90fe-f2c6-4f92-b095-35f25e52baa5"},"_deposit":{"created_by":7,"id":"2003781","owners":[7],"pid":{"revision_id":0,"type":"depid","value":"2003781"},"status":"published"},"_oai":{"id":"oai:tokushima-u.repo.nii.ac.jp:02003781","sets":["1713829974766:1713854682253:1716422254859:1716424837977","1713853213384:1713853629798"]},"author_link":["392","1573","1581"],"item_10001_alternative_title_1":{"attribute_name":"タイトル別表記","attribute_value_mlt":[{"subitem_alternative_title":"Molecular characterization of NADPH oxidase1 expressed on large intestinal epithelial cells","subitem_alternative_title_language":"en"}]},"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2003-04-25","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1-2","bibliographicPageEnd":"46","bibliographicPageStart":"45","bibliographicVolumeNumber":"59","bibliographic_titles":[{"bibliographic_title":"四国医学雑誌","bibliographic_titleLang":"ja"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"NADPH oxidase 1 (Nox1) is an isozyme of gp91-phox predominantly expressed in the human colon. In this study, we have established primary cultures of guinea pig large intestinal epithelial cells (LIEC). A great majority of the cultured cells (>90%) was surface mucous cells containing periodic acid-Schiff reaction-positive granules. Vimentin-positive fibroblasts were <1%, and macrophages were not contaminated. LIEC spontaneously produced superoxide anion (O2 －) at about 160 nmol/mg protein/h. O2 －-dependent formation of blue formazan particles from nitroblue tetrazolium was observed only on surface of mucous-producing cells, and these cells expressed Nox1 protein at plasma membrane and in the cytoplasm. They expressed p67-phox, p22-phox, and rac1, but not gp91-phox, p47-phox, p40-phox, and rac 2. Immunohistochemistry showed that Nox1, p 67-phox, and p 22-phox were predominantly expressed in surface mucous cells of human and guinea pig colonic mucosa. Human colon cancer cell lines (Caco2, T84, and HT29 cells) expressed Nox1, p22-phox, and rac1, but not the other NADPH components. These cells secreted O2 － at <5 nmol/mg protein/h. Caco2 cells possessed Toll-like receptor 5, and flagellin (FliC) from Salmonella enteritidis phosphorylated transforming growth factor-β-activated kinase 1 (TAK1) and TAK1-binding protein 1, and significantly up-regulated O2 － production. These results suggest that Nox1 expressed in colonic epithelial cells may regulate interactions between pathogenic bacteria and epithelial cells for host defense.  ","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"徳島医学会","subitem_publisher_language":"ja"}]},"item_10001_source_id_9":{"attribute_name":"収録物ID","attribute_value_mlt":[{"subitem_source_identifier":"00373699","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"AN00102041","subitem_source_identifier_type":"NCID"}]},"item_10001_version_type_20":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_1715043197608":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"桑野, 由紀","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"392","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"河原, 司","creatorNameLang":"ja"},{"creatorName":"カワハラ, ツカサ","creatorNameLang":"ja-Kana"},{"creatorName":"Kawahara, Tsukasa","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"児玉, 七重","creatorNameLang":"ja"},{"creatorName":"コダマ, ナナエ","creatorNameLang":"ja-Kana"},{"creatorName":"Kodama, Nanae","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"仲井, 沙織","creatorNameLang":"ja"},{"creatorName":"ナカイ, サオリ","creatorNameLang":"ja-Kana"},{"creatorName":"Nakai, Saori","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"岸, 恭一","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"1573","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"六反, 一仁","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"1581","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2017-11-06"}],"displaytype":"detail","filename":"sam_59_1-2_45.pdf","filesize":[{"value":"19.4 KB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://tokushima-u.repo.nii.ac.jp/record/2003781/files/sam_59_1-2_45.pdf"},"version_id":"fcab35d6-c813-4742-87af-cbef2a96c8b5"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Nox1","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"large intestinal epithelial cells","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Toll-like receptor 5","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"flagellin","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"other","resourceuri":"http://purl.org/coar/resource_type/c_1843"}]},"item_title":"大腸上皮細胞に発現するNADPH oxidase1（Nox1）の分子特性","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"大腸上皮細胞に発現するNADPH oxidase1（Nox1）の分子特性","subitem_title_language":"ja"},{"subitem_title":"ダイチョウ ジョウヒ サイボウ ニ ハツゲン スル NADPH oxidase1 Nox1 ノ ブンシ トクセイ","subitem_title_language":"ja-Kana"}]},"item_type_id":"40001","owner":"7","path":["1713853629798","1716424837977"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2017-11-06"},"publish_date":"2017-11-06","publish_status":"0","recid":"2003781","relation_version_is_last":true,"title":["大腸上皮細胞に発現するNADPH oxidase1（Nox1）の分子特性"],"weko_creator_id":"7","weko_shared_id":-1},"updated":"2024-10-28T02:24:18.876826+00:00"}