{"created":"2024-10-28T02:31:33.367998+00:00","id":2003878,"links":{},"metadata":{"_buckets":{"deposit":"6a8e6925-68f3-453e-93cf-6172ec4a4d7c"},"_deposit":{"created_by":7,"id":"2003878","owners":[7],"pid":{"revision_id":0,"type":"depid","value":"2003878"},"status":"published"},"_oai":{"id":"oai:tokushima-u.repo.nii.ac.jp:02003878","sets":["1713853213384:1713853295607"]},"author_link":["1117","1526","173"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2003","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1-2","bibliographicPageEnd":"86","bibliographicPageStart":"78","bibliographicVolumeNumber":"50","bibliographic_titles":[{"bibliographic_title":"The journal of medical investigation : JMI","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Esp-1/testisin, a serine protease abundantly expressed in human and mouse testis, is presumed to play an important role in the process of spermatogenesis and fertilization. In this study, we cloned an esp-1/testisin cDNA from rats, and analyzed its expression and tissue distribution. The isolated cDNA consisted of 1099 nucleotides with a single open reading frame encoding 328 amino acids and an expected molecular mass of 36.6 kDa. The deduced amino acid sequence of rat Esp-1/Testisin had 89% and 62% identity with its murine and human counterparts, respectively, and appeared to be a trypsin-type serine protease with a hydrophobic region at the C-terminus. By quantitative real-time polymerase chain reaction analysis, rat esp-1/testisin mRNA was predominantly expressed in testis, as in human and mouse. However, its immunohistochemical distribution was predominantly in the elongated spermatids at steps 12 to 19, and not in the primary spermatocytes and round spermatids. This different distribution profile suggests that Esp-1/Testisin plays a role in species-specific proteolytic events during spermatogenesis and fertilization.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_source_id_9":{"attribute_name":"収録物ID","attribute_value_mlt":[{"subitem_source_identifier":"13431420","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"AA11166929","subitem_source_identifier_type":"NCID"}]},"item_10001_version_type_20":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_1715043197608":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access"}]},"item_1723180141928":{"attribute_name":"EID","attribute_value_mlt":[{"subitem_identifier_type":"URI","subitem_identifier_uri":"154898"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"ナカムラ, ヤスオ","creatorNameLang":"ja"},{"creatorName":"ナカムラ, ヤスオ","creatorNameLang":"ja-Kana"},{"creatorName":"Nakamura, Yasuo","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"イノウエ, マサヒロ","creatorNameLang":"ja"},{"creatorName":"イノウエ, マサヒロ","creatorNameLang":"ja-Kana"},{"creatorName":"Inoue, Masahiro","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"奥村, 裕司","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"1117","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"シオタ, マユミ","creatorNameLang":"ja"},{"creatorName":"シオタ, マユミ","creatorNameLang":"ja-Kana"},{"creatorName":"Shiota, Mayumi","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"西川, 舞","creatorNameLang":"ja"},{"creatorName":"ニシカワ, マイ","creatorNameLang":"ja-Kana"},{"creatorName":"Nishikawa, Mai","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"荒瀬, 誠治","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"1526","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"木戸, 博","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"173","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2017-11-21"}],"displaytype":"detail","filename":"jmi_50_1-2_78.pdf","filesize":[{"value":"543 KB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://tokushima-u.repo.nii.ac.jp/record/2003878/files/jmi_50_1-2_78.pdf"},"version_id":"ac63c262-af72-42dd-9e63-1c11566b6437"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"serine protease","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"testis","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"elongated spermatids","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"spermatogenesis","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"fertilization","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Cloning, expression analysis, and tissue distribution of esp-1/testisin, a membrane-type serine protease from the rat","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Cloning, expression analysis, and tissue distribution of esp-1/testisin, a membrane-type serine protease from the rat","subitem_title_language":"en"}]},"item_type_id":"40001","owner":"7","path":["1713853295607"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2017-11-21"},"publish_date":"2017-11-21","publish_status":"0","recid":"2003878","relation_version_is_last":true,"title":["Cloning, expression analysis, and tissue distribution of esp-1/testisin, a membrane-type serine protease from the rat"],"weko_creator_id":"7","weko_shared_id":-1},"updated":"2024-10-28T02:31:42.447967+00:00"}