{"created":"2024-10-28T02:37:23.584560+00:00","id":2003955,"links":{},"metadata":{"_buckets":{"deposit":"1937d6ea-b280-4573-8e7c-fc683951f64e"},"_deposit":{"created_by":7,"id":"2003955","owners":[7],"pid":{"revision_id":0,"type":"depid","value":"2003955"},"status":"published"},"_oai":{"id":"oai:tokushima-u.repo.nii.ac.jp:02003955","sets":["1713853213384:1713853295607"]},"author_link":["1617","1616","1596"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2005-02","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1-2","bibliographicPageEnd":"73","bibliographicPageStart":"65","bibliographicVolumeNumber":"52","bibliographic_titles":[{"bibliographic_title":"The journal of medical investigation : JMI","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Asiatic acid is a pentacyclic triterpene contained inmedicinal plants. The cytotoxic effect of this compound and its augmentative effect on the anticancer drug irinotecan hydrochloride (CPT-11) were investigated in the human colon adenocarcinoma cell lineHT-29. Asiatic acid dose-dependently showed cytotoxicity in HT-29 cells. DNA fragmentation, annexin-positive apoptotic cells, andcaspase-3 activation were observed in a dose-dependent manner. Acaspase-3 inhibitor suppressed the DNA ladder formation in a concentration-dependent manner. Bcl-2 and Bcl-xL proteins were decreased by asiatic acid treatment. These results indicate that asiatic acid induced apoptosis inHT-29 cells viacaspase-3activation.Cytotoxic effectsof combined treatment with CPT-11 and asiatic acid on HT-29 cells were further examined. Simultaneous treatment or sequential exposure first to asiatic acid and then to CPT-11 showed an additive effect. Synergism was observed when cells were first exposed to CPT-11 and then to asiatic acid. These results suggest that asiatic acid can be used as an agent for increasing sensitivity of colon cancer cells to treatment with CPT-11 or as an agent for reducing adverse effects of CPT-11.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_source_id_9":{"attribute_name":"収録物ID","attribute_value_mlt":[{"subitem_source_identifier":"13431420","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"AA11166929","subitem_source_identifier_type":"NCID"}]},"item_10001_version_type_20":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_1715043197608":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access"}]},"item_1722929371688":{"attribute_name":"出版社版DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_language":"ja","subitem_relation_name_text":"10.2152/jmi.52.65"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.2152/jmi.52.65","subitem_relation_type_select":"DOI"}}]},"item_1723180141928":{"attribute_name":"EID","attribute_value_mlt":[{"subitem_identifier_type":"URI","subitem_identifier_uri":"128058"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Bunpo, Piyawan","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"片岡, 佳子","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"1617","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"有持, 秀喜","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"1616","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"中山, 治之","creatorNameLang":"ja"},{"creatorName":"ナカヤマ, ハルユキ","creatorNameLang":"ja-Kana"},{"creatorName":"Nakayama, Haruyuki","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"桑原, 知巳","creatorNameLang":"ja"},{"creatorName":"クワハラ, トモミ","creatorNameLang":"ja-Kana"},{"creatorName":"Kuwahara, Tomomi","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Vinitketkumnuen, Usanee","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"大西, 克成","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"1596","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2018-01-23"}],"displaytype":"detail","filename":"jmi_52_1-2_65.pdf","filesize":[{"value":"682 KB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://tokushima-u.repo.nii.ac.jp/record/2003955/files/jmi_52_1-2_65.pdf"},"version_id":"5d05e684-d6a3-43b2-92f3-86a83495dbba"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"asiatic acid","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"apoptosis","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"CPT-11","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"combination","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"cytotoxicity","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Inhibitory effects of asiatic acid and CPT-11 on growth of HT-29 cells","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Inhibitory effects of asiatic acid and CPT-11 on growth of HT-29 cells","subitem_title_language":"en"}]},"item_type_id":"40001","owner":"7","path":["1713853295607"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2018-01-23"},"publish_date":"2018-01-23","publish_status":"0","recid":"2003955","relation_version_is_last":true,"title":["Inhibitory effects of asiatic acid and CPT-11 on growth of HT-29 cells"],"weko_creator_id":"7","weko_shared_id":-1},"updated":"2024-10-28T02:37:32.994870+00:00"}