{"created":"2024-10-28T02:57:21.322912+00:00","id":2004200,"links":{},"metadata":{"_buckets":{"deposit":"f84623c8-5a34-485e-a3a9-d70ec28fffc6"},"_deposit":{"created_by":7,"id":"2004200","owners":[7],"pid":{"revision_id":0,"type":"depid","value":"2004200"},"status":"published"},"_oai":{"id":"oai:tokushima-u.repo.nii.ac.jp:02004200","sets":["1713829974766:1713833414474:1716250871165:1716256966674","1713853213384:1713853297799"]},"author_link":["1046"],"item_10001_alternative_title_1":{"attribute_name":"タイトル別表記","attribute_value_mlt":[{"subitem_alternative_title":"Lipocalin function of E.coli zinT (yodA) protein.","subitem_alternative_title_language":"en"}]},"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2015-10","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"4","bibliographicPageEnd":"47","bibliographicPageStart":"35","bibliographicVolumeNumber":"29","bibliographic_titles":[{"bibliographic_title":"自然科学研究","bibliographic_titleLang":"ja"},{"bibliographic_title":"Natural Science Research","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":" 重金属やアミトロール(AT)で発現誘導され、大腸菌ストレス応答タンパク質の一種であるzinT (yodA)は、一次構造比較やX線結晶構造解析による三次元立体構造などから、C末端領域に金属結合部位を持つこと、ABC transporter やlipocalinファミリーと一部相同なアミノ酸配列を持つことが明らかとなっているが、zinTの金属以外の低分子結合能については報告がない。そこで本研究では、大腸菌zinTタンパク質の種々の物質との結合能を、野生型zinTおよびN末端22残基欠損体(ΔN22)を用いて検討した。\n まず、zinTの分子内TyrまたはTrp蛍光を指標として、種々の物質を添加に伴う蛍光強度の減少を測定した。低分子化合物としては、(1)ATおよびdNTP(2) 脂肪酸および脂質5種、(3) 複素式化合物3種、(4)芳香族化合物3種を検討し、zinTに対しそれぞれ添加し、30℃、10分間保温後、蛍光スペクトルを測定した。その結果、化合物添加によりzinTの最大蛍光強度は濃度依存的に減少し、特にdNTP, Qurcetinおよび8-ANSで顕著に見られた。また、野生型とΔN22において、蛍光減少の濃度依存性に顕著な差異は見られなかった。以上より、zinTは疎水性低分子化合物結合能(lipocalin活性)を有し、N末端領域のlipocalin活性に対する寄与は少ないことが明らかとなった。","subitem_description_language":"ja","subitem_description_type":"Abstract"}]},"item_10001_source_id_9":{"attribute_name":"収録物ID","attribute_value_mlt":[{"subitem_source_identifier":"09146385","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"AN10065859","subitem_source_identifier_type":"NCID"}]},"item_10001_version_type_20":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_1715043197608":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open 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由依子","creatorNameLang":"ja"},{"creatorName":"ハンノ, ユイコ","creatorNameLang":"ja-Kana"},{"creatorName":"Hanno, Yuiko","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"酒井, 勇太","creatorNameLang":"ja"},{"creatorName":"サカイ, ハヤタ","creatorNameLang":"ja-Kana"},{"creatorName":"Sakai, Hayata","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"伊藤, 寛人","creatorNameLang":"ja"},{"creatorName":"イトウ, ヒロト","creatorNameLang":"ja-Kana"},{"creatorName":"Itoh, Hiroto","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"広瀬（柴垣）, 明佳","creatorNameLang":"ja"},{"creatorName":"ヒロセ シバガキ, ハルカ","creatorNameLang":"ja-Kana"},{"creatorName":"Shibagaki-Hirose, Haruka","creatorNameLang":"en"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"佐藤, 高則","creatorNameLang":"ja"},{"creatorName":"サトウ, タカノリ","creatorNameLang":"ja-Kana"},{"creatorName":"Satoh, Takanori","creatorNameLang":"en"}],"familyNames":[{"familyName":"佐藤","familyNameLang":"ja"},{"familyName":"サトウ","familyNameLang":"ja-Kana"},{"familyName":"Satoh","familyNameLang":"en"}],"givenNames":[{"givenName":"高則","givenNameLang":"ja"},{"givenName":"タカノリ","givenNameLang":"ja-Kana"},{"givenName":"Takanori","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1046","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"30276026","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://nrid.nii.ac.jp/ja/search/?qm=30276026"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2018-01-29"}],"displaytype":"detail","filename":"nsr_29_4_35.pdf","filesize":[{"value":"1.22 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