{"created":"2024-10-28T02:58:10.392851+00:00","id":2004210,"links":{},"metadata":{"_buckets":{"deposit":"0ee521a7-fbd8-4883-8fa4-d6262eb4f090"},"_deposit":{"created_by":7,"id":"2004210","owners":[7],"pid":{"revision_id":0,"type":"depid","value":"2004210"},"status":"published"},"_oai":{"id":"oai:tokushima-u.repo.nii.ac.jp:02004210","sets":["1713853213384:1713853295607"]},"author_link":["1242","1032"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2016-10-28","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"12","bibliographicPageEnd":"M2981","bibliographicPageStart":"M2977","bibliographicVolumeNumber":"81","bibliographic_titles":[{"bibliographic_title":"Journal of Food Science","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"We screened foods containing indigestible ingredients in the ability to adsorb Shiga toxin (Stx). When 5 mg of foods and dietary fibers such as dry vegetables and inulin were mixed and incubated with 0.5 mL of Stx solution (100 ng/mL) containing 0.5% bovine serum albumin, both Stx1 and Stx2 seemed to be adsorbed by only a fermented food, natto (a traditional Japanese food prepared from steamed soybeans by the biological action of Bacillus subtilis). We purified the Stx-adsorbing substance from natto by extraction with H 2 O, acid treatment, Proteinase K treatment, and an ion exchange chromatography. The purified substance showed an average molecular mass of about 600 kDa. We identified it as poly-γ -glutamate (PGA) by amino acid analysis of its hydrolysate and peptide analysis after its treatment with Proteinase K. Purified PGA (MW: molecular weight = about 600 kDa) was considered to adsorb both Stx1 and Stx2 when we separated adsorbed and unadsorbed Stxs (MW = about 72 kDa) by an ultrafiltration method with a centrifugal filter unit (MWCO: molecular weight cut-off = 100 K). However, PGA with the ability to adsorb Stx was an insoluble form precipitated in the filter unit during centrifugation. PGA precipitated beyond the saturated density was also confirmed to well adsorb both Stx1 and Stx2 by an equilibrated dialysis method. To the best of our knowledge, this is the 1st report on food-adsorbing Stx.   ","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Wiley","subitem_publisher_language":"en"}]},"item_10001_rights_15":{"attribute_name":"権利情報","attribute_value_mlt":[{"subitem_rights":"©2016 Institute of Food Technologists®","subitem_rights_language":"en"}]},"item_10001_source_id_9":{"attribute_name":"収録物ID","attribute_value_mlt":[{"subitem_source_identifier":"17503841","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"00221147","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"AA12164893","subitem_source_identifier_type":"NCID"},{"subitem_source_identifier":"AA00698289","subitem_source_identifier_type":"NCID"}]},"item_10001_version_type_20":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_1715043197608":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access"}]},"item_1722929371688":{"attribute_name":"出版社版DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_language":"ja","subitem_relation_name_text":"10.1111/1750-3841.13540"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1111/1750-3841.13540","subitem_relation_type_select":"DOI"}}]},"item_1723180141928":{"attribute_name":"EID","attribute_value_mlt":[{"subitem_identifier_type":"URI","subitem_identifier_uri":"321763"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"ゴトウ, ツキエ","creatorNameLang":"ja"},{"creatorName":"ゴトウ, ツキエ","creatorNameLang":"ja-Kana"},{"creatorName":"Goto, Tsukie","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"ツジ, マキコ","creatorNameLang":"ja"},{"creatorName":"ツジ, マキコ","creatorNameLang":"ja-Kana"},{"creatorName":"Tsuji, Makiko","creatorNameLang":"en"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"金丸, 芳","creatorNameLang":"ja"},{"creatorName":"カネマル, カヲリ","creatorNameLang":"ja-Kana"},{"creatorName":"Kanemaru, Kaori","creatorNameLang":"en"}],"familyNames":[{"familyName":"金丸","familyNameLang":"ja"},{"familyName":"カネマル","familyNameLang":"ja-Kana"},{"familyName":"Kanemaru","familyNameLang":"en"}],"givenNames":[{"givenName":"芳","givenNameLang":"ja"},{"givenName":"カヲリ","givenNameLang":"ja-Kana"},{"givenName":"Kaori","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1032","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"00243676","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://nrid.nii.ac.jp/ja/search/?qm=00243676"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"横井川, 久己男","creatorNameLang":"ja"},{"creatorName":"ヨコイガワ, クミオ","creatorNameLang":"ja-Kana"},{"creatorName":"Yokoigawa, Kumio","creatorNameLang":"en"}],"familyNames":[{"familyName":"横井川","familyNameLang":"ja"},{"familyName":"ヨコイガワ","familyNameLang":"ja-Kana"},{"familyName":"Yokoigawa","familyNameLang":"en"}],"givenNames":[{"givenName":"久己男","givenNameLang":"ja"},{"givenName":"クミオ","givenNameLang":"ja-Kana"},{"givenName":"Kumio","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1242","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"82339/profile-ja.html","nameIdentifierScheme":"徳島大学 教育研究者総覧","nameIdentifierURI":"http://pub2.db.tokushima-u.ac.jp/ERD/person/82339/profile-ja.html"},{"nameIdentifier":"60230637","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://nrid.nii.ac.jp/ja/search/?qm=60230637"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2018-02-02"}],"displaytype":"detail","filename":"jfs_81_12_2977.pdf","filesize":[{"value":"708 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Precipitated","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Adsorption of Shiga Toxin to Poly-γ-Glutamate Precipitated","subitem_title_language":"en"}]},"item_type_id":"40001","owner":"7","path":["1713853295607"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2018-02-02"},"publish_date":"2018-02-02","publish_status":"0","recid":"2004210","relation_version_is_last":true,"title":["Adsorption of Shiga Toxin to Poly-γ-Glutamate Precipitated"],"weko_creator_id":"7","weko_shared_id":-1},"updated":"2025-02-10T07:50:04.106706+00:00"}