{"created":"2024-10-28T03:18:39.691956+00:00","id":2004468,"links":{},"metadata":{"_buckets":{"deposit":"79deb3fa-45a8-43ee-8b0e-b81fb76486d4"},"_deposit":{"created_by":7,"id":"2004468","owners":[7],"pid":{"revision_id":0,"type":"depid","value":"2004468"},"status":"published"},"_oai":{"id":"oai:tokushima-u.repo.nii.ac.jp:02004468","sets":["1713853213384:1713853296295:1716267878536:1716268699858"]},"author_link":["1430","1584","698","329","1574"],"item_10001_alternative_title_1":{"attribute_name":"タイトル別表記","attribute_value_mlt":[{"subitem_alternative_title":"フクロネズミ腎近位尿細管細胞におけるナトリウム依存性リン酸トランスポーターNaPi-IIcと細胞内vacuole形成の関連","subitem_alternative_title_language":"ja"},{"subitem_alternative_title":"NaPi-IIc induces vacuole formation in OK cells","subitem_alternative_title_language":"en"}]},"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2015-08","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3-4","bibliographicPageEnd":"218","bibliographicPageStart":"209","bibliographicVolumeNumber":"62","bibliographic_titles":[{"bibliographic_title":"The Journal of Medical Investigation","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"NaPi-IIc/SLC34A3 is a sodium-dependent inorganic phosphate (Pi) transporter in the renal proximal tubules and its mutations cause hereditary hypophosphatemic rickets with hypercalciuria (HHRH). In the present study, we created a specific antibody for opossum SLC34A3, NaPi-IIc (oNaPi-IIc), and analyzed its localization and regulation in opossum kidney cells (a tissue culture model of proximal tubular cells). Immunoreactive oNaPi-IIc protein levels increased during the proliferative phase and decreased during differentiation. Moreover, stimulating cell growth upregulated oNaPi-IIc protein levels, whereas suppressing cell proliferation downregulated oNaPi-IIc protein levels. Immunocytochemistry revealed that endogenous and exogenous oNaPi-IIc proteins localized at the protrusion of the plasma membrane, which is a phosphatidylinositol 4,5-bisphosphate (PIP2) rich-membrane, and at the intracellular vacuolar membrane. Exogenous NaPi-IIc also induced cellular vacuoles and localized in the plasma membrane. The ability to form vacuoles is specific to electroneutral NaPi-IIc, and not electrogenic NaPi-IIa or NaPi-IIb. In addition, mutations of NaPi-IIc (S138F and R468W) in HHRH did not cause cellular PIP2-rich vacuoles. In conclusion, our data anticipate that NaPi-IIc may regulate PIP2 production at the plasma membrane and cellular vesicle formation.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Faculty of Medicine Tokushima University","subitem_publisher_language":"en"}]},"item_10001_source_id_9":{"attribute_name":"収録物ID","attribute_value_mlt":[{"subitem_source_identifier":"13496867","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"13431420","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"AA11166929","subitem_source_identifier_type":"NCID"},{"subitem_source_identifier":"AA12022913","subitem_source_identifier_type":"NCID"}]},"item_10001_version_type_20":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_be7fb7dd8ff6fe43","subitem_version_type":"NA"}]},"item_1714461018643":{"attribute_name":"報告番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第2867号"}]},"item_1714461102074":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（栄養学）","subitem_degreename_language":"ja"}]},"item_1714461118377":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2015-07-23"}]},"item_1714461137393":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_language":"ja","subitem_degreegrantor_name":"徳島大学"}]}]},"item_1715043197608":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access"}]},"item_1718868208704":{"attribute_name":"備考","attribute_value_mlt":[{"subitem_textarea_language":"ja","subitem_textarea_value":"内容要旨・審査要旨・論文本文の公開:\n内容要旨・審査要旨 : LID201508051001.pdf\n論文本文 : LID201509301001.pdf\n本論文は，著者Yuji Shiozakiの学位論文として提出され，学位審査・授与の対象となっている。\n学位授与者所属 : 徳島大学大学院栄養生命科学教育部（人間栄養科学専攻）"}]},"item_1718868303842":{"attribute_name":"学位記番号","attribute_value_mlt":[{"subitem_text_language":"ja","subitem_text_value":"甲栄第230号"}]},"item_1722929371688":{"attribute_name":"出版社版DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_language":"ja","subitem_relation_name_text":"10.2152/jmi.62.209"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.2152/jmi.62.209","subitem_relation_type_select":"DOI"}}]},"item_1723180141928":{"attribute_name":"EID","attribute_value_mlt":[{"subitem_identifier_type":"URI","subitem_identifier_uri":"316929"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"塩﨑, 雄治","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"1430","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"瀬川, 博子","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"1584","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"オオニシ, サオリ","creatorNameLang":"ja"},{"creatorName":"オオニシ, サオリ","creatorNameLang":"ja-Kana"},{"creatorName":"Ohnishi, Saori","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"オオイ, アキコ","creatorNameLang":"ja"},{"creatorName":"オオイ, アキコ","creatorNameLang":"ja-Kana"},{"creatorName":"Ohi, Akiko","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"イトウ, ミキコ","creatorNameLang":"ja"},{"creatorName":"イトウ, ミキコ","creatorNameLang":"ja-Kana"},{"creatorName":"Ito, Mikiko","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"金子, 一郎","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"698","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"キド, シンスケ","creatorNameLang":"ja"},{"creatorName":"キド, シンスケ","creatorNameLang":"ja-Kana"},{"creatorName":"Kido, Shinsuke","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"辰巳, 佐和子","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"329","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"宮本, 賢一","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"1574","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2016-08-06"}],"displaytype":"detail","filename":"LID201508051001.pdf","filesize":[{"value":"187 KB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"objectType":"abstract","url":"https://tokushima-u.repo.nii.ac.jp/record/2004468/files/LID201508051001.pdf"},"version_id":"f1d91df8-a72a-467d-a86f-ffc10cbe409a"},{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2016-10-01"}],"displaytype":"detail","filename":"LID201509301001.pdf","filesize":[{"value":"1.56 MB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://tokushima-u.repo.nii.ac.jp/record/2004468/files/LID201509301001.pdf"},"version_id":"4b806b60-9d58-4a52-8cd5-59d84ac0ef1f"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"proximal tubule","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"SLC34A3","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"opossum kidney cells","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"proliferation","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"vacuole","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Relationship between sodium-dependent phosphate transporter (NaPi-IIc) function and cellular vacuole formation in opossum kidney cells","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Relationship between sodium-dependent phosphate transporter (NaPi-IIc) function and cellular vacuole formation in opossum kidney cells","subitem_title_language":"en"}]},"item_type_id":"40001","owner":"7","path":["1716268699858"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2018-05-22"},"publish_date":"2018-05-22","publish_status":"0","recid":"2004468","relation_version_is_last":true,"title":["Relationship between sodium-dependent phosphate transporter (NaPi-IIc) function and cellular vacuole formation in opossum kidney cells"],"weko_creator_id":"7","weko_shared_id":-1},"updated":"2024-10-28T03:18:51.341350+00:00"}