{"created":"2024-10-28T03:18:56.451631+00:00","id":2004471,"links":{},"metadata":{"_buckets":{"deposit":"c1ea333b-e043-47df-9ef8-1773d65681b7"},"_deposit":{"created_by":7,"id":"2004471","owners":[7],"pid":{"revision_id":0,"type":"depid","value":"2004471"},"status":"published"},"_oai":{"id":"oai:tokushima-u.repo.nii.ac.jp:02004471","sets":["1713829974766:1713854681789:1716364515583:1716365500378","1713853213384:1713853295607"]},"author_link":["1594","483","921","246"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2015-08","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3-4","bibliographicPageEnd":"232","bibliographicPageStart":"228","bibliographicVolumeNumber":"62","bibliographic_titles":[{"bibliographic_title":"The Journal of Medical Investigation","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"We recently constructed two rhesus macaque-tropic human immunodeficiency virus type 1 (HIV-1 rmt) clones with CXCR-4 or CCR5 tropism, but a CCR5-tropic HIV-1rmt clone grew more poorly than a CXCR4topic clone. It has been demonstrated that interaction between viral Gag-matrix (MA) and Env-gp41 cytoplasmic tail is important for virion-incorporation of Env. Concordantly, Gag-MA mutations (62QR and 66SR) that rescue defects in virion-incorporation of Env/viral replication were reported. In this study, we analyzed effects of these Gag-MA mutations on R5-tropic HIV-1rmt replication potentials. While introduction of 62QR into three HIV-1rmt clones tested reduced their multi-cycle replication ability in rhesus lymphocytes or abolish single-cycle infectivity for luciferase reporter cells, three R5-tropic HIV-1rmt clones carrying 66SR exhibited similar growth kinetics to those of their parental clones. One such clone, 66SR+5gtu, appeared to induce stronger cytopathic effects than parental clone 5gtu. We therefore investigated effects of 66SR mutation on viral replication in more detail. Single-cycle infectivity of 66SR+5gtu was enhanced relative to that of 5gtu, but 66SR+5gtu virion production was significantly decreased compared to the 5gtu level. Gag-MA 66SR mutation may be useful to improve growth potentials of the R5-tropic HIV-1rmt clones.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Faculty of Medicine Tokushima 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enhances viral single-cycle infectivity of R5-tropic HIV-1rmt","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Single-amino acid mutation 66SR in Gag-matrix enhances viral single-cycle infectivity of R5-tropic HIV-1rmt","subitem_title_language":"en"}]},"item_type_id":"40001","owner":"7","path":["1713853295607","1716365500378"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2018-05-22"},"publish_date":"2018-05-22","publish_status":"0","recid":"2004471","relation_version_is_last":true,"title":["Single-amino acid mutation 66SR in Gag-matrix enhances viral single-cycle infectivity of R5-tropic HIV-1rmt"],"weko_creator_id":"7","weko_shared_id":-1},"updated":"2025-01-29T08:42:10.231782+00:00"}