{"created":"2024-10-28T03:21:43.677196+00:00","id":2004507,"links":{},"metadata":{"_buckets":{"deposit":"dccc2acd-3af7-4677-b436-56ebbdccecf7"},"_deposit":{"created_by":7,"id":"2004507","owners":[7],"pid":{"revision_id":0,"type":"depid","value":"2004507"},"status":"published"},"_oai":{"id":"oai:tokushima-u.repo.nii.ac.jp:02004507","sets":["1713829974766:1713854681789:1716364517711:1716365817658","1713853213384:1713853295607"]},"author_link":["1577","337"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2009-08","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3-4","bibliographicPageEnd":"149","bibliographicPageStart":"142","bibliographicVolumeNumber":"56","bibliographic_titles":[{"bibliographic_title":"The Journal of Medical Investigation","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Despite the popularity of 3T3-L1 adipocytes as a model system of adipocytes in vivo, they do not carry all of the cellular functions of adipocytes in vivo. In this study, we investigated the effect of extracellular matrix (ECM) rigidity on insulin signal transduction in 3T3-L1 adipocytes. On 250 Pa polyacrylamide gel (soft gel) laminated with a mixture of collagen type 1 and fibronectin, whose rigidity matches that of adipose tissue, expression of the insulin receptor, IRS-1 and AKT was upregulated and their insulin-stimulated phosphorylation was enhanced. Furthermore, the expression of GLUT1 was downregulated, whereas the expression of GLUT4 was unaffected as ECM rigidity decreased. Insulin-stimulated GLUT4 recruitment to the plasma membrane was significantly enhanced in cells seeded on soft gel. These results suggest that adjusting the ECM rigidity to that of adipose tissue augments insulin signaling in 3T3-L1 adipocytes and enhances insulin-stimulated GLUT4 recruitment to the plasma membrane.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Faculty of Medicine Tokushima University","subitem_publisher_language":"en"}]},"item_10001_source_id_9":{"attribute_name":"収録物ID","attribute_value_mlt":[{"subitem_source_identifier":"13496867","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"13431420","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"AA11166929","subitem_source_identifier_type":"NCID"},{"subitem_source_identifier":"AA12022913","subitem_source_identifier_type":"NCID"}]},"item_10001_version_type_20":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_1715043197608":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access"}]},"item_1722929371688":{"attribute_name":"出版社版DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_language":"ja","subitem_relation_name_text":"10.2152/jmi.56.142"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.2152/jmi.56.142","subitem_relation_type_select":"DOI"}}]},"item_1723180141928":{"attribute_name":"EID","attribute_value_mlt":[{"subitem_identifier_type":"URI","subitem_identifier_uri":"195208"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Li, Qinkai","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"保坂, 利男","creatorNameLang":"ja"},{"creatorName":"ホサカ, トシオ","creatorNameLang":"ja-Kana"},{"creatorName":"Hosaka, Toshio","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Jambaldorj, Bayasglan","creatorNameLang":"en"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"中屋, 豊","creatorNameLang":"ja"},{"creatorName":"ナカヤ, ユタカ","creatorNameLang":"ja-Kana"},{"creatorName":"Nakaya, Yutaka","creatorNameLang":"en"}],"familyNames":[{"familyName":"中屋","familyNameLang":"ja"},{"familyName":"ナカヤ","familyNameLang":"ja-Kana"},{"familyName":"Nakaya","familyNameLang":"en"}],"givenNames":[{"givenName":"豊","givenNameLang":"ja"},{"givenName":"ユタカ","givenNameLang":"ja-Kana"},{"givenName":"Yutaka","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1577","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"60130/profile-ja.html","nameIdentifierScheme":"徳島大学 教育研究者総覧","nameIdentifierURI":"http://pub2.db.tokushima-u.ac.jp/ERD/person/60130/profile-ja.html"},{"nameIdentifier":"50136222","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://nrid.nii.ac.jp/ja/search/?qm=50136222"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"船木, 真理","creatorNameLang":"ja"},{"creatorName":"フナキ, マコト","creatorNameLang":"ja-Kana"},{"creatorName":"Funaki, Makoto","creatorNameLang":"en"}],"familyNames":[{"familyName":"船木","familyNameLang":"ja"},{"familyName":"フナキ","familyNameLang":"ja-Kana"},{"familyName":"Funaki","familyNameLang":"en"}],"givenNames":[{"givenName":"真理","givenNameLang":"ja"},{"givenName":"マコト","givenNameLang":"ja-Kana"},{"givenName":"Makoto","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"337","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"175544/profile-ja.html","nameIdentifierScheme":"徳島大学 教育研究者総覧","nameIdentifierURI":"http://pub2.db.tokushima-u.ac.jp/ERD/person/175544/profile-ja.html"},{"nameIdentifier":"10467821","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://nrid.nii.ac.jp/ja/search/?qm=10467821"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2018-05-22"}],"displaytype":"detail","filename":"jmi_56_3-4_142.pdf","filesize":[{"value":"1.12 MB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://tokushima-u.repo.nii.ac.jp/record/2004507/files/jmi_56_3-4_142.pdf"},"version_id":"73f84eb0-c01d-4bbd-9d8c-0d1fc451bd60"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"extracellular matrix","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"rigidity","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"insulin","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"GLUT4","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"3T3-L1 adipocytes","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Extracellular matrix with the rigidity of adipose tissue helps 3T3-L1 adipocytes maintain insulin responsiveness","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Extracellular matrix with the rigidity of adipose tissue helps 3T3-L1 adipocytes maintain insulin responsiveness","subitem_title_language":"en"}]},"item_type_id":"40001","owner":"7","path":["1713853295607","1716365817658"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2018-05-22"},"publish_date":"2018-05-22","publish_status":"0","recid":"2004507","relation_version_is_last":true,"title":["Extracellular matrix with the rigidity of adipose tissue helps 3T3-L1 adipocytes maintain insulin responsiveness"],"weko_creator_id":"7","weko_shared_id":-1},"updated":"2025-01-28T07:58:42.121166+00:00"}