{"created":"2024-10-28T03:29:56.343730+00:00","id":2004620,"links":{},"metadata":{"_buckets":{"deposit":"b62b22fe-0cec-49f6-a9e9-67e343ba69af"},"_deposit":{"created_by":7,"id":"2004620","owners":[7],"pid":{"revision_id":0,"type":"depid","value":"2004620"},"status":"published"},"_oai":{"id":"oai:tokushima-u.repo.nii.ac.jp:02004620","sets":["1713853213384:1713853296295:1716267876683:1716268551628"]},"author_link":["1572","349","852","359","175","756","287"],"control_number":"2004620","item_10001_alternative_title_1":{"attribute_name":"タイトル別表記","attribute_value_mlt":[{"subitem_alternative_title":"c-KITを標的としたGastrointestinal stromal tumorsに対する近赤外蛍光イメージングとレーザー照射の新しいセラノスティクス","subitem_alternative_title_language":"ja"}]},"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2018-03-21","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"9","bibliographicPageEnd":"2328","bibliographicPageStart":"2313","bibliographicVolumeNumber":"8","bibliographic_titles":[{"bibliographic_title":"Theranostics","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"It is difficult to distinguish gastrointestinal stromal tumors (GISTs) from other types of submucosal tumors under conventional gastrointestinal endoscopy. We aimed to detect GISTs by molecular fluorescence imaging using a near-infrared (NIR) photosensitizer (IR700)-conjugated anti-c-KIT antibody and to treat GISTs by photoimmunotherapy with NIR irradiation as a non-invasive theranostic procedure. We also investigated the therapeutic mechanisms.\nMethods: Human GIST cell lines GIST-T1 and GIST-882M were incubated with IR700-conjugated anti-c-KIT antibody, IR700-12A8, and observed by confocal laser microscopy. Mice with GIST-T1 xenografts or rats with orthotopic xenografts were injected with IR700-12A8 or AF488-conjugated antibody, and observed under IVIS or autofluorescence imaging (AFI) endoscopy. GIST cells were treated with IR700-12A8 and NIR light in vitro and vivo, and cell viability, histology and apoptosis were evaluated.\nResults: Strong red fluorescence of IR700-12A8 was observed on the cell membrane of GIST cells and was gradually internalized into the cytoplasm. Tumor-specific accumulation of IR700-12A8 was observed in GIST-T1 xenografts in mice. Under AFI endoscopy, a strong fluorescence signal was observed in orthotopic GIST xenografts in rats through the normal mucosa covering the tumor. The percentage of dead cells significantly increased in a light-dose-dependent manner and both acute necrotic and late apoptotic cell death was observed with annexin/PI staining. Cleaved PARP expression was significantly increased after IR700-12A8-mediated NIR irradiation, which was almost completely reversed by NaN3. All xenograft tumors (7/7) immediately regressed and 4/7 tumors completely disappeared after IR700-12A8-mediated NIR irradiation. Histologic assessment and TUNEL staining revealed apoptosis in the tumors.\nConclusion: NIR fluorescence imaging using IR700-12A8 and subsequent NIR irradiation could be a very effective theranostic technology for GIST, the underlying mechanism of which appears to involve acute necrosis and supposedly late apoptosis induced by singlet oxygen.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Ivyspring International Publisher","subitem_publisher_language":"en"}]},"item_10001_rights_15":{"attribute_name":"権利情報","attribute_value_mlt":[{"subitem_rights":"© 2018 Ivyspring International Publisher. This is an open access article distributed under the terms of the Creative Commons Attribution (CC BY-NC) license. See http://ivyspring.com/terms for full terms and conditions.","subitem_rights_language":"en"}]},"item_10001_source_id_9":{"attribute_name":"収録物ID","attribute_value_mlt":[{"subitem_source_identifier":"18387640","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_be7fb7dd8ff6fe43","subitem_version_type":"NA"}]},"item_1714461018643":{"attribute_name":"報告番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第3130号"}]},"item_1714461102074":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（医学）","subitem_degreename_language":"ja"}]},"item_1714461118377":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2018-03-23"}]},"item_1714461137393":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_language":"ja","subitem_degreegrantor_name":"徳島大学"}]}]},"item_1715043197608":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access"}]},"item_1718868208704":{"attribute_name":"備考","attribute_value_mlt":[{"subitem_textarea_language":"ja","subitem_textarea_value":"内容要旨・審査要旨・論文本文の公開\n本論文は，著者Shota Fujimotoの学位論文として提出され，学位審査・授与の対象となっている。\n学位授与者所属 : 徳島大学大学院医科学教育部（医学専攻）"}]},"item_1718868303842":{"attribute_name":"学位記番号","attribute_value_mlt":[{"subitem_text_language":"ja","subitem_text_value":"甲医第1364号"}]},"item_1722929371688":{"attribute_name":"出版社版DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_language":"ja","subitem_relation_name_text":"10.7150/thno.22027"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.7150/thno.22027","subitem_relation_type_select":"DOI"}}]},"item_1723180141928":{"attribute_name":"EID","attribute_value_mlt":[{"subitem_identifier_type":"URI","subitem_identifier_uri":"335640"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"藤本, 将太","creatorNameLang":"ja"},{"creatorName":"フジモト, ショウタ","creatorNameLang":"ja-Kana"},{"creatorName":"Fujimoto, Shota","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"六車, 直樹","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"1572","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"岡本, 耕一","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"349","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"クリハラ, タケシ","creatorNameLang":"ja"},{"creatorName":"クリハラ, タケシ","creatorNameLang":"ja-Kana"},{"creatorName":"Kurihara, Takeshi","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"佐藤, 康史","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"852","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"ミヤモト, ヨシヒコ","creatorNameLang":"ja"},{"creatorName":"ミヤモト, ヨシヒコ","creatorNameLang":"ja-Kana"},{"creatorName":"Miyamoto, Yoshihiko","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"北村, 晋志","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"359","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"宮本, 弘志","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"175","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"タグチ, タカヒロ","creatorNameLang":"ja"},{"creatorName":"タグチ, タカヒロ","creatorNameLang":"ja-Kana"},{"creatorName":"Taguchi, Takahiro","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"常山, 幸一","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"756","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"高山, 哲治","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"287","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2018-04-19"}],"displaytype":"detail","filename":"k3130_abstract_review.pdf","filesize":[{"value":"335 KB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"objectType":"abstract","url":"https://tokushima-u.repo.nii.ac.jp/record/2004620/files/k3130_abstract_review.pdf"},"version_id":"e60d87b5-a723-44d8-bed8-4b07b9f749f7"},{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2018-04-19"}],"displaytype":"detail","filename":"k3130_fulltext.pdf","filesize":[{"value":"2.94 MB"}],"format":"application/pdf","licensetype":"license_3","mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://tokushima-u.repo.nii.ac.jp/record/2004620/files/k3130_fulltext.pdf"},"version_id":"8d72dd5c-c0be-49a9-b148-12ba79ed0cbf"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"gastrointestinal stromal tumors (GIST)","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"c-KIT","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"fluorescence endoscopy","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"near-infrared photoimmunotherapy","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"A Novel Theranostic Combination of Near-infrared Fluorescence Imaging and Laser Irradiation Targeting c-KIT for Gastrointestinal Stromal Tumors","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"A Novel Theranostic Combination of Near-infrared Fluorescence Imaging and Laser Irradiation Targeting c-KIT for Gastrointestinal Stromal Tumors","subitem_title_language":"en"}]},"item_type_id":"40001","owner":"7","path":["1716268551628"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2018-04-19"},"publish_date":"2018-04-19","publish_status":"0","recid":"2004620","relation_version_is_last":true,"title":["A Novel Theranostic Combination of Near-infrared Fluorescence Imaging and Laser Irradiation Targeting c-KIT for Gastrointestinal Stromal Tumors"],"weko_creator_id":"7","weko_shared_id":-1},"updated":"2024-12-13T05:19:32.415878+00:00"}