{"created":"2024-10-28T05:29:42.602415+00:00","id":2004648,"links":{},"metadata":{"_buckets":{"deposit":"a8e71918-5ada-445f-a3f2-dea7827de0ba"},"_deposit":{"created_by":7,"id":"2004648","owners":[7],"pid":{"revision_id":0,"type":"depid","value":"2004648"},"status":"published"},"_oai":{"id":"oai:tokushima-u.repo.nii.ac.jp:02004648","sets":["1713829974766:1713854681789:1716364517711:1716365816891","1713853213384:1713853629798"]},"author_link":[],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2009-12","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"Supplement","bibliographicPageEnd":"354","bibliographicPageStart":"354","bibliographicVolumeNumber":"56","bibliographic_titles":[{"bibliographic_title":"The Journal of Medical Investigation","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The ductal system of the exocrine pancreas produces HCO3--rich fluid in response to secretin and other stimuli. HCO3- efflux across the luminal membrane is mediated by a Cl--HCO3- exchanger operating in parallel with the cystic fibrosis transmembrane conductance regulator (CFTR) Cl- channel. Basolateral K+ channels provide an exit pathway for K+ and play a vital role in maintaining the membrane potential, which is a crucial component of the driving force for anion secretion. Measurements of membrane potential with intracellular microelectrodes suggested that Ba2+-sensitive K+ conductance accounts for more than 60% of the total basolateral ionic conductance in resting ducts (1). To identify the Ba2+-sensitive K+ channels, we isolated ducts from normal rat pancreas by collagenase digestion. We first demonstrated that the ducts did not express a vascular endothelial marker PECAM-1 (platelet/endothelial cell adhesion molecule-1), but expressed cytokeratin 20, a marker of duct cells (2), using immunofluorescent staining. In addition, monoclonal anti-CFTR antibody was detected near the luminal membrane of these cells. In cell-attached single-channel recordings, we observed three types of K+ channels on basolateral membrane in unstimulated duct cells. The 40 pS K+ channels are likely to mediate whole-cell inwardly rectifying K+ (Kir) currents, which were blocked by extracellular Ba2+ in a voltage-dependent manner. The properties of 90 pS and 170 pS K+ channels are similar to those of Ca2+-activated K+ channels. We then identified Kir2.0 and SK4/IK1 (intermediate conductance Ca2+-activated K+ channel) subunits as molecular candidates of the K+ channels using RT-PCR analysis. The present results suggest that these subunits may mediate native K+ currents in resting duct cells. Further functional studies with specific blockers are required to evaluate which of these K+ channels contribute to the resting membrane potential and might be involved in HCO3- secretion.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Faculty of Medicine Tokushima University","subitem_publisher_language":"en"}]},"item_10001_source_id_9":{"attribute_name":"収録物ID","attribute_value_mlt":[{"subitem_source_identifier":"13496867","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"13431420","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"AA11166929","subitem_source_identifier_type":"NCID"},{"subitem_source_identifier":"AA12022913","subitem_source_identifier_type":"NCID"}]},"item_10001_version_type_20":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_1715043197608":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access"}]},"item_1722929371688":{"attribute_name":"出版社版DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_language":"ja","subitem_relation_name_text":"10.2152/jmi.56.354"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.2152/jmi.56.354","subitem_relation_type_select":"DOI"}}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"ハヤシ, ミキオ","creatorNameLang":"ja"},{"creatorName":"ハヤシ, ミキオ","creatorNameLang":"ja-Kana"},{"creatorName":"Hayashi, Mikio","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"マツダ, ヒロコ","creatorNameLang":"ja"},{"creatorName":"マツダ, ヒロコ","creatorNameLang":"ja-Kana"},{"creatorName":"Matsuda, Hiroko","creatorNameLang":"en"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2018-06-22"}],"displaytype":"detail","filename":"jmi_56_suppl_354.pdf","filesize":[{"value":"38.8 KB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://tokushima-u.repo.nii.ac.jp/record/2004648/files/jmi_56_suppl_354.pdf"},"version_id":"243d36c9-760d-4bad-a521-70106c4a261a"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"pancreatic duct","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"potassium channel","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"patch clamp","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"other","resourceuri":"http://purl.org/coar/resource_type/c_1843"}]},"item_title":"K+ channels on resting duct cells from rat pancreas","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"K+ channels on resting duct cells from rat pancreas","subitem_title_language":"en"}]},"item_type_id":"40001","owner":"7","path":["1713853629798","1716365816891"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2018-06-22"},"publish_date":"2018-06-22","publish_status":"0","recid":"2004648","relation_version_is_last":true,"title":["K+ channels on resting duct cells from rat pancreas"],"weko_creator_id":"7","weko_shared_id":-1},"updated":"2025-01-20T04:53:53.422274+00:00"}