{"created":"2024-10-28T06:09:24.384348+00:00","id":2005157,"links":{},"metadata":{"_buckets":{"deposit":"a4d7a4a8-c5b3-45fd-9400-fad6b925c663"},"_deposit":{"created_by":7,"id":"2005157","owners":[7],"pid":{"revision_id":0,"type":"depid","value":"2005157"},"status":"published"},"_oai":{"id":"oai:tokushima-u.repo.nii.ac.jp:02005157","sets":["1713853213384:1713853295607"]},"author_link":["1211","1256","1705","463"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2016-05-10","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"24","bibliographicPageStart":"19","bibliographicVolumeNumber":"69","bibliographic_titles":[{"bibliographic_title":"Archives of Oral Biology","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Objective: Porphyromonas gingivalis (P. gingivalis) is a pathogen involved in periodontal disease. Recently, periodontal disease has been demonstrated to increase the risk of developing diabetes mellitus, although the molecular mechanism is not fully understood. Forkhead box protein O1 (FoxO1) is a transcriptional factor that regulates gluconeogenesis in the liver. Gluconeogenesis is a key process in the induction of diabetes mellitus; however, little is known regarding the relationship between periodontal disease and gluconeogenesis. In this study, to investigate whether periodontal disease influences hepatic gluconeogenesis, we examined the effects of P. gingivalis on the phosphorylation and translocation of FoxO1 in insulin-induced human hepatocytes.\nDesign: The human hepatocyte HepG2 was treated with insulin and Akt and FoxO1 phosphorylation was detected by western blot analysis. The localization of phosphorylated FoxO1 was detected by immunocytochemistry and western blot analysis. HepG2 cells were treated with SNAP26b-tagged P. gingivalis (SNAP-P. g.) before insulin stimulation, and then the changes in Akt and FoxO1 were determined by western blot analysis and immunocytochemistry.\nResults: Insulin (100 nM) induced FoxO1 phosphorylation 60 min after treatment in HepG2 cells. Phosphorylated FoxO1 translocated to the cytoplasm. SNAP-P.g. internalized into HepG2 cells and decreased Akt and FoxO1 phosphorylation induced by insulin. The effect of insulin on FoxO1 translocation was also attenuated by SNAP-P.g.\nConclusions: Our study shows that P. gingivalis decreases the phosphorylation and translocation of FoxO induced by insulin in HepG2 cells. Our results suggest that periodontal disease may increase hepatic gluconeogenesis by reducing the effects of insulin on FoxO1.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier","subitem_publisher_language":"en"}]},"item_10001_rights_15":{"attribute_name":"権利情報","attribute_value_mlt":[{"subitem_rights":"© 2016. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/","subitem_rights_language":"en"}]},"item_10001_source_id_9":{"attribute_name":"収録物ID","attribute_value_mlt":[{"subitem_source_identifier":"00039969","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"AA00548457","subitem_source_identifier_type":"NCID"},{"subitem_source_identifier":"AA11521918","subitem_source_identifier_type":"NCID"}]},"item_10001_version_type_20":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_1715043197608":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access"}]},"item_1722929371688":{"attribute_name":"出版社版DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_language":"ja","subitem_relation_name_text":"10.1016/j.archoralbio.2016.05.010"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1016/j.archoralbio.2016.05.010","subitem_relation_type_select":"DOI"}}]},"item_1723180141928":{"attribute_name":"EID","attribute_value_mlt":[{"subitem_identifier_type":"URI","subitem_identifier_uri":"313121"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"タカムラ, ハルナ","creatorNameLang":"ja"},{"creatorName":"タカムラ, ハルナ","creatorNameLang":"ja-Kana"},{"creatorName":"Takamura, Haruna","creatorNameLang":"en"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"吉田, 賀弥","creatorNameLang":"ja"},{"creatorName":"ヨシダ, カヤ","creatorNameLang":"ja-Kana"},{"creatorName":"Yoshida, Kaya","creatorNameLang":"en"}],"familyNames":[{"familyName":"吉田","familyNameLang":"ja"},{"familyName":"ヨシダ","familyNameLang":"ja-Kana"},{"familyName":"Yoshida","familyNameLang":"en"}],"givenNames":[{"givenName":"賀弥","givenNameLang":"ja"},{"givenName":"カヤ","givenNameLang":"ja-Kana"},{"givenName":"Kaya","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1211","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"73175/profile-ja.html","nameIdentifierScheme":"徳島大学 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Hirohiko","creatorNameLang":"en"}],"familyNames":[{"familyName":"岡村","familyNameLang":"ja"},{"familyName":"オカムラ","familyNameLang":"ja-Kana"},{"familyName":"Okamura","familyNameLang":"en"}],"givenNames":[{"givenName":"裕彦","givenNameLang":"ja"},{"givenName":"ヒロヒコ","givenNameLang":"ja-Kana"},{"givenName":"Hirohiko","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1256","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"20380024","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://nrid.nii.ac.jp/ja/search/?qm=20380024"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"藤原, 奈津美","creatorNameLang":"ja"},{"creatorName":"フジワラ, ナツミ","creatorNameLang":"ja-Kana"},{"creatorName":"Fujiwara, Natsumi","creatorNameLang":"en"}],"familyNames":[{"familyName":"藤原","familyNameLang":"ja"},{"familyName":"フジワラ","familyNameLang":"ja-Kana"},{"familyName":"Fujiwara","familyNameLang":"en"}],"givenNames":[{"givenName":"奈津美","givenNameLang":"ja"},{"givenName":"ナツミ","givenNameLang":"ja-Kana"},{"givenName":"Natsumi","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"463","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"213034/profile-ja.html","nameIdentifierScheme":"徳島大学 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Kazumi","creatorNameLang":"en"}],"familyNames":[{"familyName":"尾崎","familyNameLang":"ja"},{"familyName":"オザキ","familyNameLang":"ja-Kana"},{"familyName":"Ozaki","familyNameLang":"en"}],"givenNames":[{"givenName":"和美","givenNameLang":"ja"},{"givenName":"カズミ","givenNameLang":"ja-Kana"},{"givenName":"Kazumi","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1705","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"60420/profile-ja.html","nameIdentifierScheme":"徳島大学 教育研究者総覧","nameIdentifierURI":"http://pub2.db.tokushima-u.ac.jp/ERD/person/60420/profile-ja.html"},{"nameIdentifier":"90214121","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://nrid.nii.ac.jp/ja/search/?qm=90214121"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2018-07-25"}],"displaytype":"detail","filename":"archoralbio_69_19.pdf","filesize":[{"value":"969 KB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://tokushima-u.repo.nii.ac.jp/record/2005157/files/archoralbio_69_19.pdf"},"version_id":"1bceb798-32f6-4379-880e-0027cfa2470e"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"diabetes mellitus","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"forkhead box protein O1 (FoxO1)","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"periodontal disease","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Porphyromonas gingivalis","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal 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