{"created":"2024-10-28T07:31:47.374337+00:00","id":2005479,"links":{},"metadata":{"_buckets":{"deposit":"84f62f38-eb85-4cb4-9c66-587596f0d1d2"},"_deposit":{"created_by":7,"id":"2005479","owners":[7],"pid":{"revision_id":0,"type":"depid","value":"2005479"},"status":"published"},"_oai":{"id":"oai:tokushima-u.repo.nii.ac.jp:02005479","sets":["1713853213384:1713853295607"]},"author_link":["1674","1651"],"item_10001_alternative_title_1":{"attribute_name":"タイトル別表記","attribute_value_mlt":[{"subitem_alternative_title":"OMV Production in Bacteroides fragilis","subitem_alternative_title_language":"en"}]},"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2016-02-09","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageStart":"e0148887","bibliographicVolumeNumber":"11","bibliographic_titles":[{"bibliographic_title":"PLOS ONE","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Phase changes in Bacteroides fragilis, a member of the human colonic microbiota, mediate variations in a vast array of cell surface molecules, such as capsular polysaccharides and outer membrane proteins through DNA inversion. The results of the present study show that outer membrane vesicle (OMV) formation in this anaerobe is also controlled by DNA inversions at two distantly localized promoters, IVp-I and IVp-II that are associated with extracellular polysaccharide biosynthesis and the expression of outer membrane proteins. These promoter inversions are mediated by a single tyrosine recombinase encoded by BF2766 (orthologous to tsr19 in strain NCTC9343) in B. fragilis YCH46, which is located near IVp-I. A series of BF2766 mutants were constructed in which the two promoters were locked in different configurations (IVp-I/IVp-II = ON/ON, OFF/OFF, ON/OFF or OFF/ON). ON/ON B. fragilis mutants exhibited hypervesiculating, whereas the other mutants formed only a trace amount of OMVs. The hypervesiculating ON/ON mutants showed higher resistance to treatment with bile, LL-37, and human β-defensin 2. Incubation of wild-type cells with 5% bile increased the population of cells with the ON/ON genotype. These results indicate that B. fragilis regulates the formation of OMVs through DNA inversions at two distantly related promoter regions in response to membrane stress, although the mechanism underlying the interplay between the two regions controlled by the invertible promoters remains unknown.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"PLOS","subitem_publisher_language":"en"}]},"item_10001_rights_15":{"attribute_name":"権利情報","attribute_value_mlt":[{"subitem_rights":"Copyright: © 2016 Nakayama-Imaohji et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.","subitem_rights_language":"en"}]},"item_10001_source_id_9":{"attribute_name":"収録物ID","attribute_value_mlt":[{"subitem_source_identifier":"19326203","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_1715043197608":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access"}]},"item_1722929371688":{"attribute_name":"出版社版DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_language":"ja","subitem_relation_name_text":"10.1371/journal.pone.0148887"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1371/journal.pone.0148887","subitem_relation_type_select":"DOI"}}]},"item_1723180141928":{"attribute_name":"EID","attribute_value_mlt":[{"subitem_identifier_type":"URI","subitem_identifier_uri":"315272"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"ナカヤマ イマオオジ, ハルユキ","creatorNameLang":"ja"},{"creatorName":"ナカヤマ イマオオジ, ハルユキ","creatorNameLang":"ja-Kana"},{"creatorName":"Nakayama-Imaohji, Haruyuki","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"弘田, 克彦","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"1674","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"ヤマサキ, ヒサシ","creatorNameLang":"ja"},{"creatorName":"ヤマサキ, ヒサシ","creatorNameLang":"ja-Kana"},{"creatorName":"Yamasaki, Hisashi","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"ヨネダ, サオリ","creatorNameLang":"ja"},{"creatorName":"ヨネダ, サオリ","creatorNameLang":"ja-Kana"},{"creatorName":"Yoneda, Saori","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"ナリヤ, ヒロフミ","creatorNameLang":"ja"},{"creatorName":"ナリヤ, ヒロフミ","creatorNameLang":"ja-Kana"},{"creatorName":"Nariya, Hirofumi","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"スズキ, モトオ","creatorNameLang":"ja"},{"creatorName":"スズキ, モトオ","creatorNameLang":"ja-Kana"},{"creatorName":"Suzuki, Motoo","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Secher, Thomas","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"三宅, 洋一郎","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"1651","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Oswald, Eric","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"ハヤシ, テツヤ","creatorNameLang":"ja"},{"creatorName":"ハヤシ, テツヤ","creatorNameLang":"ja-Kana"},{"creatorName":"Hayashi, Tetsuya","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"クワハラ, トモミ","creatorNameLang":"ja"},{"creatorName":"クワハラ, トモミ","creatorNameLang":"ja-Kana"},{"creatorName":"Kuwahara, Tomomi","creatorNameLang":"en"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2018-11-12"}],"displaytype":"detail","filename":"pone_11_2_e0148887.pdf","filesize":[{"value":"2.04 MB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://tokushima-u.repo.nii.ac.jp/record/2005479/files/pone_11_2_e0148887.pdf"},"version_id":"159ea8ff-3811-453b-b77f-90b223f4d990"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"DNA Inversion Regulates Outer Membrane Vesicle Production in Bacteroides fragilis","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"DNA Inversion Regulates Outer Membrane Vesicle Production in Bacteroides fragilis","subitem_title_language":"en"}]},"item_type_id":"40001","owner":"7","path":["1713853295607"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2018-11-12"},"publish_date":"2018-11-12","publish_status":"0","recid":"2005479","relation_version_is_last":true,"title":["DNA Inversion Regulates Outer Membrane Vesicle Production in Bacteroides fragilis"],"weko_creator_id":"7","weko_shared_id":-1},"updated":"2024-10-28T07:31:59.082059+00:00"}