{"created":"2024-10-28T07:35:28.976063+00:00","id":2005522,"links":{},"metadata":{"_buckets":{"deposit":"0b039e26-8c51-48bd-933f-e4a042a0f053"},"_deposit":{"created_by":7,"id":"2005522","owners":[7],"pid":{"revision_id":0,"type":"depid","value":"2005522"},"status":"published"},"_oai":{"id":"oai:tokushima-u.repo.nii.ac.jp:02005522","sets":["1713853213384:1713853295607"]},"author_link":["500","478","305"],"control_number":"2005522","item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2016-09-16","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"32886","bibliographicVolumeNumber":"6","bibliographic_titles":[{"bibliographic_title":"Scientific Reports","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The integrated stress response (ISR) is a cytoprotective pathway initiated upon phosphorylation of the eukaryotic translation initiation factor 2 (eIF2α) residue designated serine-51, which is critical for translational control in response to various stress conditions. Four eIF2α kinases, namely heme-regulated inhibitor (HRI), protein kinase R (PKR), PKR-like endoplasmic reticulum kinase, (PERK) and general control non-depressible 2 (GCN2), have been identified thus far, and they are known to be activated by heme depletion, viral infection, endoplasmic reticulum stress, and amino acid starvation, respectively. Because eIF2α is phosphorylated under various stress conditions, the existence of an additional eIF2α kinase has been suggested. To validate the existence of the unidentified eIF2α kinase, we constructed an eIF2α kinase quadruple knockout cells (4KO cells) in which the four known eIF2α kinase genes were deleted using the CRISPR/Cas9-mediated genome editing. Phosphorylation of eIF2α was completely abolished in the 4KO cells by various stress stimulations. Our data suggests that the four known eIF2α kinases are sufficient for ISR and that there are no additional eIF2α kinases in vertebrates.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Springer Nature","subitem_publisher_language":"en"}]},"item_10001_rights_15":{"attribute_name":"権利情報","attribute_value_mlt":[{"subitem_rights":"© The Author(s) 2016 This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/","subitem_rights_language":"en"}]},"item_10001_source_id_9":{"attribute_name":"収録物ID","attribute_value_mlt":[{"subitem_source_identifier":"20452322","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_1715043197608":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access"}]},"item_1718868208704":{"attribute_name":"備考","attribute_value_mlt":[{"subitem_textarea_language":"ja","subitem_textarea_value":"Supplementary Information : srep_6_32886_s1.pdf"}]},"item_1722929371688":{"attribute_name":"出版社版DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_language":"ja","subitem_relation_name_text":"10.1038/srep32886"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1038/srep32886","subitem_relation_type_select":"DOI"}}]},"item_1723180141928":{"attribute_name":"EID","attribute_value_mlt":[{"subitem_identifier_type":"URI","subitem_identifier_uri":"330607"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"タニウチ, シュウスケ","creatorNameLang":"ja"},{"creatorName":"タニウチ, シュウスケ","creatorNameLang":"ja-Kana"},{"creatorName":"Taniuchi, Shusuke","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"三宅, 雅人","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"500","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"ツガワ, カズエ","creatorNameLang":"ja"},{"creatorName":"ツガワ, カズエ","creatorNameLang":"ja-Kana"},{"creatorName":"Tsugawa, Kazue","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"親泊, 美帆","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"478","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"親泊, 政一","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"305","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2018-11-12"}],"displaytype":"detail","filename":"srep_6_32886.pdf","filesize":[{"value":"3.39 MB"}],"format":"application/pdf","licensetype":"license_0","mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://tokushima-u.repo.nii.ac.jp/record/2005522/files/srep_6_32886.pdf"},"version_id":"43b77805-374e-4dc1-b929-1675ea9ecf5b"},{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2018-11-12"}],"displaytype":"detail","filename":"srep_6_32886_s1.pdf","filesize":[{"value":"491 KB"}],"format":"application/pdf","licensetype":"license_0","mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://tokushima-u.repo.nii.ac.jp/record/2005522/files/srep_6_32886_s1.pdf"},"version_id":"faedb618-1861-48d2-ab01-0747e277c2d9"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Integrated stress response of vertebrates is regulated by four eIF2α kinases","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Integrated stress response of vertebrates is regulated by four eIF2α kinases","subitem_title_language":"en"}]},"item_type_id":"40001","owner":"7","path":["1713853295607"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2018-11-12"},"publish_date":"2018-11-12","publish_status":"0","recid":"2005522","relation_version_is_last":true,"title":["Integrated stress response of vertebrates is regulated by four eIF2α kinases"],"weko_creator_id":"7","weko_shared_id":-1},"updated":"2024-12-15T23:39:40.796631+00:00"}