{"created":"2024-10-28T08:11:14.266344+00:00","id":2006031,"links":{},"metadata":{"_buckets":{"deposit":"8f46e531-5d6c-4f3e-9a51-12802c5303e1"},"_deposit":{"created_by":7,"id":"2006031","owners":[7],"pid":{"revision_id":0,"type":"depid","value":"2006031"},"status":"published"},"_oai":{"id":"oai:tokushima-u.repo.nii.ac.jp:02006031","sets":["1713853213384:1713853296295:1716267878536:1716268699858"]},"author_link":["761","1156","1578","1585","1583"],"control_number":"2006031","item_10001_alternative_title_1":{"attribute_name":"タイトル別表記","attribute_value_mlt":[{"subitem_alternative_title":"ステロール応答領域結合タンパク質1は腎近位尿細管細胞において25-水酸化ビタミンD3 24-水酸化酵素遺伝子の転写を活性化する","subitem_alternative_title_language":"ja"}]},"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2018-04-17","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"282","bibliographicPageStart":"275","bibliographicVolumeNumber":"500","bibliographic_titles":[{"bibliographic_title":"Biochemical and Biophysical Research Communications","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The physiological activity of the steroid derived hormone vitamin D is regulated by several enzymatic steps. Both 25-hydroxy vitamin D3 1α-hydroxylase (CYP27B1) and 25-hydroxyvitamin D3 24-hydroxylase (CYP24A1) modulate blood levels of 1,25-dihydroxyvitamin D3, an activated form of vitamin D. We previously demonstrated that CYP27B1 expression was trans-activated by sterol regulatory element binding protein 1 (SREBP1), although whether SREBP1 also regulates CYP24A1 transcription was unclear.  Here we investigated the ability of SREBP1 to affect CYP24A1 transcription. In a luciferase reporter assay, mouse and human CYP24A1 promoter activity was strongly activated by SREBP1 in opossum kidney proximal tubular cells (OK-P). Three putative SREs (pSREs) were found in the mouse Cyp24a1 gene promoter and the SREBP1 protein showed specific binding to the pSRE1 element in EMSAs. Site-directed mutagenesis of the pSRE1 element strongly decreased SREBP1-mediated Cyp24a1 gene transcription.  Moreover, siRNA-mediated SREBP1 knock-down repressed CYP24A1 expression in human renal proximal tubular epithelial cells (HKC-8). In animal studies, mice given various doses of thyroid hormone (T3) showed dose-dependent reductions in renal Srebp1c and Cyp24a1 mRNA levels. Taken together, our results suggest that SREBP1 trans-activates CYP24A1 expression through SREBP binding elements present  in the promoter.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier","subitem_publisher_language":"en"}]},"item_10001_source_id_9":{"attribute_name":"収録物ID","attribute_value_mlt":[{"subitem_source_identifier":"0006291X","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"AA00564395","subitem_source_identifier_type":"NCID"},{"subitem_source_identifier":"AA11542044","subitem_source_identifier_type":"NCID"}]},"item_10001_version_type_20":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_be7fb7dd8ff6fe43","subitem_version_type":"NA"}]},"item_1714461018643":{"attribute_name":"報告番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第3221号"}]},"item_1714461102074":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（栄養学）","subitem_degreename_language":"ja"}]},"item_1714461118377":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2018-09-27"}]},"item_1714461137393":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_language":"ja","subitem_degreegrantor_name":"徳島大学"}]}]},"item_1715043197608":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access"}]},"item_1718868208704":{"attribute_name":"備考","attribute_value_mlt":[{"subitem_textarea_language":"ja","subitem_textarea_value":"内容要旨・審査要旨・論文本文の公開\n本論文は, 著者Tomohiro Kagawaの学位論文として提出され, 学位審査・授与の対象となっている。\n学位授与者所属 : 徳島大学大学院栄養生命科学教育部（人間栄養科学専攻）"}]},"item_1718868303842":{"attribute_name":"学位記番号","attribute_value_mlt":[{"subitem_text_language":"ja","subitem_text_value":"甲栄第261号"}]},"item_1722929371688":{"attribute_name":"出版社版DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_language":"ja","subitem_relation_name_text":"10.1016/j.bbrc.2018.04.058"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1016/j.bbrc.2018.04.058","subitem_relation_type_select":"DOI"}}]},"item_1723180141928":{"attribute_name":"EID","attribute_value_mlt":[{"subitem_identifier_type":"URI","subitem_identifier_uri":"343155"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"香川, 知博","creatorNameLang":"ja"},{"creatorName":"カガワ, トモヒロ","creatorNameLang":"ja-Kana"},{"creatorName":"Kagawa, Tomohiro","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"コウザイ, ミナ","creatorNameLang":"ja"},{"creatorName":"コウザイ, ミナ","creatorNameLang":"ja-Kana"},{"creatorName":"Kozai, Mina","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"増田, 真志","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"761","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"原田, 永勝","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"1156","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"ナカハシ, オトキ","creatorNameLang":"ja"},{"creatorName":"ナカハシ, オトキ","creatorNameLang":"ja-Kana"},{"creatorName":"Nakahashi, Otoki","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"タジリ, マリ","creatorNameLang":"ja"},{"creatorName":"タジリ, マリ","creatorNameLang":"ja-Kana"},{"creatorName":"Tajiri, Mari","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"ヨシカワ, リョウヘイ","creatorNameLang":"ja"},{"creatorName":"ヨシカワ, リョウヘイ","creatorNameLang":"ja-Kana"},{"creatorName":"Yoshikawa, Ryouhei","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"ナカオ, マリ","creatorNameLang":"ja"},{"creatorName":"ナカオ, マリ","creatorNameLang":"ja-Kana"},{"creatorName":"Nakao, Mari","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"タケイ, ユウイチロウ","creatorNameLang":"ja"},{"creatorName":"タケイ, ユウイチロウ","creatorNameLang":"ja-Kana"},{"creatorName":"Takei, Yuichiro","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"イワノ, マサユキ","creatorNameLang":"ja"},{"creatorName":"イワノ, マサユキ","creatorNameLang":"ja-Kana"},{"creatorName":"Iwano, Masayuki","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"武田, 英二","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"1578","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"竹谷, 豊","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"1585","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"山本, 浩範","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"1583","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2018-12-20"}],"displaytype":"detail","filename":"k3221_abstract_review.pdf","filesize":[{"value":"199 KB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"objectType":"abstract","url":"https://tokushima-u.repo.nii.ac.jp/record/2006031/files/k3221_abstract_review.pdf"},"version_id":"5f250b82-fb89-4886-8f09-0293c5457bd6"},{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2018-12-20"}],"displaytype":"detail","filename":"k3221_fulltext.pdf","filesize":[{"value":"1.24 MB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://tokushima-u.repo.nii.ac.jp/record/2006031/files/k3221_fulltext.pdf"},"version_id":"afb7ce61-1bc7-491e-954b-826fddf4cce5"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"CYP24A1","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"SREBP1","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Thyroid hormone","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Transcriptional regulation","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Vitamin D","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Sterol regulatory element binding protein 1 trans-activates 25-hydroxy vitamin D3 24-hydroxylase gene expression in renal proximal tubular cells","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Sterol regulatory element binding protein 1 trans-activates 25-hydroxy vitamin D3 24-hydroxylase gene expression in renal proximal tubular cells","subitem_title_language":"en"}]},"item_type_id":"40001","owner":"7","path":["1716268699858"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2018-12-20"},"publish_date":"2018-12-20","publish_status":"0","recid":"2006031","relation_version_is_last":true,"title":["Sterol regulatory element binding protein 1 trans-activates 25-hydroxy vitamin D3 24-hydroxylase gene expression in renal proximal tubular cells"],"weko_creator_id":"7","weko_shared_id":-1},"updated":"2024-12-26T05:08:54.231721+00:00"}