{"created":"2024-10-28T08:24:39.773333+00:00","id":2006195,"links":{},"metadata":{"_buckets":{"deposit":"362f860a-9113-4f87-b4c1-668abaf30bf1"},"_deposit":{"created_by":7,"id":"2006195","owners":[7],"pid":{"revision_id":0,"type":"depid","value":"2006195"},"status":"published"},"_oai":{"id":"oai:tokushima-u.repo.nii.ac.jp:02006195","sets":["1713853213384:1713853295607"]},"author_link":["775"],"control_number":"2006195","item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2019-02-20","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"20","bibliographicVolumeNumber":"2","bibliographic_titles":[{"bibliographic_title":"Communications Chemistry","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Lipid transfer proteins mediate inter-organelle transport of membrane lipids at organelle contact sites in cells, playing fundamental roles in the lipidome and membrane biogenesis in eukaryotes. We previously developed a ceramide-mimetic compound as a potent inhibitor of the ceramide transport protein CERT. Here we develop CERT inhibitors with structures unrelated to ceramide. To this aim, we identify a seed compound with no ceramide-like structure but with the capability of forming a hydrogen-bonding network in the ceramide-binding START domain, by virtual screening of ~3 × 106 compounds. We also establish a surface plasmon resonance-based system to directly determine the affinity of compounds for the START domain. Then, we subject the seed compound to a series of in silico docking simulations, efficient chemical synthesis, affinity analysis, protein-ligand co-crystallography, and various in vivo assays. This strategy allows us to obtain ceramide-unrelated compounds that potently inhibited the function of CERT in human cultured cells.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Springer Nature","subitem_publisher_language":"en"}]},"item_10001_rights_15":{"attribute_name":"権利情報","attribute_value_mlt":[{"subitem_rights":"© 2019 The Author(s). This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.","subitem_rights_language":"en"}]},"item_10001_source_id_9":{"attribute_name":"収録物ID","attribute_value_mlt":[{"subitem_source_identifier":"23993669","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_1715043197608":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access"}]},"item_1722929371688":{"attribute_name":"出版社版DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_language":"ja","subitem_relation_name_text":"10.1038/s42004-019-0118-3"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1038/s42004-019-0118-3","subitem_relation_type_select":"DOI"}}]},"item_1723180141928":{"attribute_name":"EID","attribute_value_mlt":[{"subitem_identifier_type":"URI","subitem_identifier_uri":"349539"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"ナカオ, ナオキ","creatorNameLang":"ja"},{"creatorName":"ナカオ, ナオキ","creatorNameLang":"ja-Kana"},{"creatorName":"Nakao, Naoki","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"上野, 雅晴","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"775","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"サカイ, ショウタ","creatorNameLang":"ja"},{"creatorName":"サカイ, ショウタ","creatorNameLang":"ja-Kana"},{"creatorName":"Sakai, Shota","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"エガワ, ダイチ","creatorNameLang":"ja"},{"creatorName":"エガワ, ダイチ","creatorNameLang":"ja-Kana"},{"creatorName":"Egawa, Daichi","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"ハンザワ, ヒロユキ","creatorNameLang":"ja"},{"creatorName":"ハンザワ, ヒロユキ","creatorNameLang":"ja-Kana"},{"creatorName":"Hanzawa, Hiroyuki","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"カワサキ, ショウヘイ","creatorNameLang":"ja"},{"creatorName":"カワサキ, ショウヘイ","creatorNameLang":"ja-Kana"},{"creatorName":"Kawasaki, Shohei","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"クマガイ, ケイゴ","creatorNameLang":"ja"},{"creatorName":"クマガイ, ケイゴ","creatorNameLang":"ja-Kana"},{"creatorName":"Kumagai, Keigo","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"スズキ, マコト","creatorNameLang":"ja"},{"creatorName":"スズキ, マコト","creatorNameLang":"ja-Kana"},{"creatorName":"Suzuki, Makoto","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"コバヤシ, シュウ","creatorNameLang":"ja"},{"creatorName":"コバヤシ, シュウ","creatorNameLang":"ja-Kana"},{"creatorName":"Kobayashi, Shu","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"ハナダ, ケンタロウ","creatorNameLang":"ja"},{"creatorName":"ハナダ, ケンタロウ","creatorNameLang":"ja-Kana"},{"creatorName":"Hanada, Kentaro","creatorNameLang":"en"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2019-02-26"}],"displaytype":"detail","filename":"commschem_2_20.pdf","filesize":[{"value":"2.37 MB"}],"format":"application/pdf","licensetype":"license_0","mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://tokushima-u.repo.nii.ac.jp/record/2006195/files/commschem_2_20.pdf"},"version_id":"0d6676e7-2dda-497e-9503-5e1ad8548861"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Natural ligand-nonmimetic inhibitors of the lipid-transfer protein CERT","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Natural ligand-nonmimetic inhibitors of the lipid-transfer protein CERT","subitem_title_language":"en"}]},"item_type_id":"40001","owner":"7","path":["1713853295607"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2019-02-26"},"publish_date":"2019-02-26","publish_status":"0","recid":"2006195","relation_version_is_last":true,"title":["Natural ligand-nonmimetic inhibitors of the lipid-transfer protein CERT"],"weko_creator_id":"7","weko_shared_id":-1},"updated":"2024-12-06T00:28:21.262607+00:00"}