{"created":"2024-10-29T00:33:06.122378+00:00","id":2006459,"links":{},"metadata":{"_buckets":{"deposit":"db7039a5-f5df-4a7b-b671-9b4fbb030408"},"_deposit":{"created_by":7,"id":"2006459","owners":[7],"pid":{"revision_id":0,"type":"depid","value":"2006459"},"status":"published"},"_oai":{"id":"oai:tokushima-u.repo.nii.ac.jp:02006459","sets":["1713853213384:1713853296295:1716267878536:1716268699858"]},"author_link":["1430","1584","698","329","1574"],"item_10001_alternative_title_1":{"attribute_name":"タイトル別表記","attribute_value_mlt":[{"subitem_alternative_title":"ヒト腎臓におけるリン代謝機構を理解するためのオポッサム腎NaPi-IIc (Na+依存性Piトランスポーター)の解析","subitem_alternative_title_language":"ja"}]},"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2018-10-13","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3","bibliographicPageEnd":"324","bibliographicPageStart":"313","bibliographicVolumeNumber":"23","bibliographic_titles":[{"bibliographic_title":"Clinical and Experimental Nephrology","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Background The role of Na+- dependent inorganic phosphate (Pi) transporters in the human kidney is not fully clarified. Hereditary hypophosphatemic rickets with hypercalciuria (HHRH) is caused by loss-of-function mutations in the IIc Na+- dependent Pi transporter (NPT2c/Npt2c/NaPi-IIc) gene. Another Na+- dependent type II transporter, (NPT2A/Npt2a/NaPi-IIa), is also important for renal Pi reabsorption in humans. In mice, Npt2c deletion does not lead to hypophosphatemia and rickets because Npt2a compensates for the impaired Pi reabsorption. To clarify the differences between mouse and human, we investigated the relation between NaPi-IIa and NaPi-IIc functions in opossum kidney (OK) cells.\nMethods We cloned NaPi-IIc from OK cells and created opossum NaPi-IIc (oNaPi-IIc) antibodies. We used oNaPi-IIc small interference (si)RNA and investigated the role of NaPi-IIc in Pi transport in OK cells.\nResults We cloned opossum kidney NaPi-IIc cDNAs encoding 622 amino acid proteins (variant1) and examined their pH- and sodium-dependency. The antibodies reacted specifically with 75-kDa and 150-kDa protein bands, and the siRNA of NaPi-IIc markedly suppressed endogenous oNaPi-IIc in OK cells. Treatment with siRNA significantly suppressed the expression of NaPi-4 (NaPi-IIa) protein and mRNA. oNaPi-IIc siRNA also suppressed Na+/H+ exchanger regulatory factor 1 expression in OK cells.\nConclusion These findings suggest that NaPi-IIc is important for the expression of NaPi-IIa (NaPi-4) protein in OK cells. Suppression of Npt2c may downregulate Npt2a function in HHRH patients.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Japanese Society of Nephrology","subitem_publisher_language":"en"},{"subitem_publisher":"Springer","subitem_publisher_language":"en"}]},"item_10001_rights_15":{"attribute_name":"権利情報","attribute_value_mlt":[{"subitem_rights":"© Japanese Society of Nephrology 2018","subitem_rights_language":"en"}]},"item_10001_source_id_9":{"attribute_name":"収録物ID","attribute_value_mlt":[{"subitem_source_identifier":"13421751","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"14377799","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"AA11126935","subitem_source_identifier_type":"NCID"}]},"item_10001_version_type_20":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_be7fb7dd8ff6fe43","subitem_version_type":"NA"}]},"item_1714461018643":{"attribute_name":"報告番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第3273号"}]},"item_1714461102074":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（栄養学）","subitem_degreename_language":"ja"}]},"item_1714461118377":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2019-03-22"}]},"item_1714461137393":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_language":"ja","subitem_degreegrantor_name":"徳島大学"}]}]},"item_1715043197608":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access"}]},"item_1718868208704":{"attribute_name":"備考","attribute_value_mlt":[{"subitem_textarea_language":"ja","subitem_textarea_value":"内容要旨・審査要旨・論文本文の公開\n本論文は，著者Toru Fujiiの学位論文として提出され，学位審査・授与の対象となっている。\nThe final publication is available at Springer via https://doi.org/10.1007/s10157-018-1653-4\n学位授与者所属 : 徳島大学大学院栄養生命科学教育部（人間栄養科学専攻）"}]},"item_1718868303842":{"attribute_name":"学位記番号","attribute_value_mlt":[{"subitem_text_language":"ja","subitem_text_value":"甲栄第262号"}]},"item_1722929371688":{"attribute_name":"出版社版DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_language":"ja","subitem_relation_name_text":"10.1007/s10157-018-1653-4"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1007/s10157-018-1653-4","subitem_relation_type_select":"DOI"}}]},"item_1723180141928":{"attribute_name":"EID","attribute_value_mlt":[{"subitem_identifier_type":"URI","subitem_identifier_uri":"355022"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"藤井, 公","creatorNameLang":"ja"},{"creatorName":"フジイ, トオル","creatorNameLang":"ja-Kana"},{"creatorName":"Fujii, Toru","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"塩﨑, 雄治","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"1430","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"瀬川, 博子","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"1584","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"ニシグチ, シオリ","creatorNameLang":"ja"},{"creatorName":"ニシグチ, シオリ","creatorNameLang":"ja-Kana"},{"creatorName":"Nishiguchi, Shiori","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"ハナザキ, アイ","creatorNameLang":"ja"},{"creatorName":"ハナザキ, アイ","creatorNameLang":"ja-Kana"},{"creatorName":"Hanazaki, Ai","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"ノグチ, ミワ","creatorNameLang":"ja"},{"creatorName":"ノグチ, ミワ","creatorNameLang":"ja-Kana"},{"creatorName":"Noguchi, Miwa","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"キリノ, ルリ","creatorNameLang":"ja"},{"creatorName":"キリノ, ルリ","creatorNameLang":"ja-Kana"},{"creatorName":"Kirino, Ruri","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"ササキ, スミレ","creatorNameLang":"ja"},{"creatorName":"ササキ, スミレ","creatorNameLang":"ja-Kana"},{"creatorName":"Sasaki, Sumire","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"タニフジ, カズヤ","creatorNameLang":"ja"},{"creatorName":"タニフジ, カズヤ","creatorNameLang":"ja-Kana"},{"creatorName":"Tanifuji, Kazuya","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"コイケ, メグミ","creatorNameLang":"ja"},{"creatorName":"コイケ, メグミ","creatorNameLang":"ja-Kana"},{"creatorName":"Koike, Megumi","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"ヨコヤマ, ミズキ","creatorNameLang":"ja"},{"creatorName":"ヨコヤマ, ミズキ","creatorNameLang":"ja-Kana"},{"creatorName":"Yokoyama, Mizuki","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"アリマ, ユウキ","creatorNameLang":"ja"},{"creatorName":"アリマ, ユウキ","creatorNameLang":"ja-Kana"},{"creatorName":"Arima, Yuki","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"金子, 一郎","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"698","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"辰巳, 佐和子","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"329","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"イトウ, ミキコ","creatorNameLang":"ja"},{"creatorName":"イトウ, ミキコ","creatorNameLang":"ja-Kana"},{"creatorName":"Ito, Mikiko","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"宮本, 賢一","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"1574","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2019-06-13"}],"displaytype":"detail","filename":"k3273_abstract_review.pdf","filesize":[{"value":"207 KB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"objectType":"abstract","url":"https://tokushima-u.repo.nii.ac.jp/record/2006459/files/k3273_abstract_review.pdf"},"version_id":"225d4628-efc1-44ef-a0d3-9625acf405b2"},{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2020-03-04"}],"displaytype":"detail","filename":"k3273_fulltext.pdf","filesize":[{"value":"6.09 MB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://tokushima-u.repo.nii.ac.jp/record/2006459/files/k3273_fulltext.pdf"},"version_id":"e376e477-0d1e-402f-932d-d7ba783d0b1b"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Phosphate","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Transporter","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Proximal tubule","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Hereditary hypophosphatemic rickets with hypercalciuria","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Analysis of opossum kidney NaPi-IIc sodium-dependent phosphate transporter to understand Pi handling in human kidney","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Analysis of opossum kidney NaPi-IIc sodium-dependent phosphate transporter to understand Pi handling in human kidney","subitem_title_language":"en"}]},"item_type_id":"40001","owner":"7","path":["1716268699858"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2019-06-13"},"publish_date":"2019-06-13","publish_status":"0","recid":"2006459","relation_version_is_last":true,"title":["Analysis of opossum kidney NaPi-IIc sodium-dependent phosphate transporter to understand Pi handling in human kidney"],"weko_creator_id":"7","weko_shared_id":-1},"updated":"2024-10-29T00:33:14.915048+00:00"}