{"created":"2024-10-29T00:33:48.646360+00:00","id":2006466,"links":{},"metadata":{"_buckets":{"deposit":"cf1f1094-8844-4d67-8d99-2bcaa300f711"},"_deposit":{"created_by":7,"id":"2006466","owners":[7],"pid":{"revision_id":0,"type":"depid","value":"2006466"},"status":"published"},"_oai":{"id":"oai:tokushima-u.repo.nii.ac.jp:02006466","sets":["1713853213384:1713853296295:1716267877313:1716268556148"]},"author_link":["827","718","859","589"],"item_10001_alternative_title_1":{"attribute_name":"タイトル別表記","attribute_value_mlt":[{"subitem_alternative_title":"TNF-α短期刺激による骨髄由来間葉系幹細胞の細胞動態解析","subitem_alternative_title_language":"ja"},{"subitem_alternative_title":"Effect of Short Term TNF-α Stimulation of BMSCs","subitem_alternative_title_language":"en"}]},"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2019-04-24","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"108","bibliographicPageStart":"99","bibliographicVolumeNumber":"28","bibliographic_titles":[{"bibliographic_title":"Journal of Hard Tissue Biology","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Bone marrow-derived mesenchymal stem cells (BMSCs) have considerable potential for self-renewal and multi-differentiation. Tumor necrosis factor-α (TNF-α) is an inflammatory cytokine and is involved in tissue regeneration during wound healing. It was already reported that cultured human dental pulp cells acquire stem cell properties following short-term stimulation by TNF-α. However, it has not been clarified if BMSCs acquire stem cell properties after TNF-α treatment. The purpose of this study was to investigate the effect of short-term stimulation with TNF-α on BMSCs. Rat BMSCs were cultured up to 60% confluence and then incubated with 1–100 ng/ml of recombinant rat TNF-α (rTNF-α) for a further 2 days. After reaching subconfluence, cells were passaged once to remove rTNF-α completely before subsequent assays. Cells in the control group were passaged without stimulation. Expression levels of Nanog and Oct4 stem cell markers were significantly increased in the rTNF-α 10 ng/ml stimulation group. rTNF-α stimulation did not affect cell proliferation compared with the control group. However, rTNF-α stimulation led to a delay in cell differentiation. This study suggests that short-term TNF-α stimulation of BMSCs significantly increased their stem cell phenotype, but delayed their osteogenic cell differentiation.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"THE SOCIETY FOR HARD TISSUE REGENERATIVE BIOLOGY","subitem_publisher_language":"en"}]},"item_10001_source_id_9":{"attribute_name":"収録物ID","attribute_value_mlt":[{"subitem_source_identifier":"13417649","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"1880828X","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"AA11074332","subitem_source_identifier_type":"NCID"}]},"item_10001_version_type_20":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_be7fb7dd8ff6fe43","subitem_version_type":"NA"}]},"item_1714461018643":{"attribute_name":"報告番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第3282号"}]},"item_1714461102074":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（歯学）","subitem_degreename_language":"ja"}]},"item_1714461118377":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2019-03-22"}]},"item_1714461137393":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_language":"ja","subitem_degreegrantor_name":"徳島大学"}]}]},"item_1715043197608":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access"}]},"item_1718868208704":{"attribute_name":"備考","attribute_value_mlt":[{"subitem_textarea_language":"ja","subitem_textarea_value":"内容要旨・審査要旨・論文本文の公開\n本論文は，著者Mio Naritaniの学位論文として提出され，学位審査・授与の対象となっている。\n学位授与者所属 : 徳島大学大学院口腔科学教育部（口腔科学専攻）"}]},"item_1718868303842":{"attribute_name":"学位記番号","attribute_value_mlt":[{"subitem_text_language":"ja","subitem_text_value":"甲口第447号"}]},"item_1722929371688":{"attribute_name":"出版社版DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_language":"ja","subitem_relation_name_text":"10.2485/jhtb.28.99"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.2485/jhtb.28.99","subitem_relation_type_select":"DOI"}}]},"item_1723180141928":{"attribute_name":"EID","attribute_value_mlt":[{"subitem_identifier_type":"URI","subitem_identifier_uri":"354302"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"成谷, 美緒","creatorNameLang":"ja"},{"creatorName":"ナリタニ, ミオ","creatorNameLang":"ja-Kana"},{"creatorName":"Naritani, Mio","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"井上, 美穂","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"827","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Raju, Resmi","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"宮城, 麻友","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"718","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"大島, 正充","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"859","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"松香, 芳三","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"589","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2019-06-13"}],"displaytype":"detail","filename":"k3282_abstract.pdf","filesize":[{"value":"166 KB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"objectType":"abstract","url":"https://tokushima-u.repo.nii.ac.jp/record/2006466/files/k3282_abstract.pdf"},"version_id":"ad32c222-d5a6-4768-a424-6a3cc14f51c1"},{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2019-06-13"}],"displaytype":"detail","filename":"k3282_review.pdf","filesize":[{"value":"41.7 KB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"objectType":"other","url":"https://tokushima-u.repo.nii.ac.jp/record/2006466/files/k3282_review.pdf"},"version_id":"cade9ace-2615-480c-b9ff-1730dab30c4d"},{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2019-06-13"}],"displaytype":"detail","filename":"k3282_fulltext.pdf","filesize":[{"value":"1.31 MB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://tokushima-u.repo.nii.ac.jp/record/2006466/files/k3282_fulltext.pdf"},"version_id":"21366054-af86-4f89-a8fc-5098f8a8e51b"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"TNF-α","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Bone marrow-derived mesenchymal stem cells","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Short-term stimulation","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Cell proliferation","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Cell differentiation","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Analysis of Bone Marrow-derived Mesenchymal Stem Cell Kinetics after Short-term Stimulation with Tumor Necrosis Factor-α (TNF-α)","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Analysis of Bone Marrow-derived Mesenchymal Stem Cell Kinetics after Short-term Stimulation with Tumor Necrosis Factor-α (TNF-α)","subitem_title_language":"en"}]},"item_type_id":"40001","owner":"7","path":["1716268556148"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2019-06-13"},"publish_date":"2019-06-13","publish_status":"0","recid":"2006466","relation_version_is_last":true,"title":["Analysis of Bone Marrow-derived Mesenchymal Stem Cell Kinetics after Short-term Stimulation with Tumor Necrosis Factor-α (TNF-α)"],"weko_creator_id":"7","weko_shared_id":-1},"updated":"2024-10-29T00:34:00.337689+00:00"}