{"created":"2024-10-29T00:36:57.780914+00:00","id":2006500,"links":{},"metadata":{"_buckets":{"deposit":"22f49daf-543f-405e-9c34-7268165aa26d"},"_deposit":{"created_by":7,"id":"2006500","owners":[7],"pid":{"revision_id":0,"type":"depid","value":"2006500"},"status":"published"},"_oai":{"id":"oai:tokushima-u.repo.nii.ac.jp:02006500","sets":["1713853213384:1713853296295:1716267876683:1716268551628"]},"author_link":["392","874","1581","546"],"item_10001_alternative_title_1":{"attribute_name":"タイトル別表記","attribute_value_mlt":[{"subitem_alternative_title":"HOXA6-HOXA5遺伝子座の新規長鎖ノンコーディングRNAは大腸癌細胞の増殖を促進する","subitem_alternative_title_language":"ja"}]},"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2019-06-03","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"532","bibliographicVolumeNumber":"19","bibliographic_titles":[{"bibliographic_title":"BMC Cancer","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Background : Homeobox A5 (HOXA5), a member of the HOX family, plays an important role in tumor development and morphogenesis, although opposite effects on tumorigenesis have been observed, depending on the tissue type. In this study, we aimed to investigate the role of a novel transcript from the HOXA6-HOXA5 locus in colon cancer tumorigenesis.\nMethods : Human colon cancer cell lines were analyzed using next generation sequencing-based targeted mRNA capture. The effects of overexpression and silencing of HOXA5 transcripts were evaluated in vitro and using a xenograft nude mouse model.\nResults : We identified three novel transcripts (HOXA5 short, long 1, and long 2) transcribed from the HOXA6-HOXA5 locus in HCT116 colon cancer cells using next generation sequencing-based targeted mRNA capture. Knockdown of HOXA5 long 1 and long 2 transcripts did not affect cell growth, while selective silencing of HOXA5 short RNA inhibited cell growth independent of HOXA5 expression. Stable overexpression of HOXA5 short RNA promoted proliferation and migration of colon cancer cell lines HCT116, DLD1, and HT-29 and accelerated tumor growth in the xenograft mouse model. In vitro translation assays suggested HOXA5 short RNA was a functional long non-coding RNA (lncRNA). Consistent with these observations, expression of HOXA5 short RNA was upregulated in advanced colon cancer tissues. Ingenuity Pathway Analysis of differentially expressed genes between HOXA5 short RNA overexpressed and silenced HCT116 cells revealed that HOXA5 short RNA preferentially modified expression of epidermal growth factor (EGF) signal-related genes. Western blot analysis demonstrated that stable overexpression of HOXA5 short RNA increased EGF receptor levels and facilitated its phosphorylation in both HCT116 cells and xenograft tumors.\nConclusions : Our results suggested that HOXA5 short RNA, a novel lncRNA, may play a crucial role in colon tumor growth through activation of EGF signaling.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"BioMed Central","subitem_publisher_language":"en"}]},"item_10001_rights_15":{"attribute_name":"権利情報","attribute_value_mlt":[{"subitem_rights":"© The Author(s). 2019 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License ( http://creativecommons.org/licenses/by/4.0/ ), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver ( http://creativecommons.org/publicdomain/zero/1.0/ ) applies to the data made available in this article, unless otherwise stated.","subitem_rights_language":"en"}]},"item_10001_source_id_9":{"attribute_name":"収録物ID","attribute_value_mlt":[{"subitem_source_identifier":"14712407","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"AA12034763","subitem_source_identifier_type":"NCID"}]},"item_10001_version_type_20":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_be7fb7dd8ff6fe43","subitem_version_type":"NA"}]},"item_1714461018643":{"attribute_name":"報告番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第3263号"}]},"item_1714461102074":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（医学）","subitem_degreename_language":"ja"}]},"item_1714461118377":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2019-03-22"}]},"item_1714461137393":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_language":"ja","subitem_degreegrantor_name":"徳島大学"}]}]},"item_1715043197608":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access"}]},"item_1718868208704":{"attribute_name":"備考","attribute_value_mlt":[{"subitem_textarea_language":"ja","subitem_textarea_value":"内容要旨・審査要旨・論文本文の公開\n本論文は，著者Saki Saijoの学位論文として提出され，学位審査・授与の対象となっている。\n学位授与者所属 : 徳島大学大学院医科学教育部（医学専攻）"}]},"item_1718868303842":{"attribute_name":"学位記番号","attribute_value_mlt":[{"subitem_text_language":"ja","subitem_text_value":"甲医第1412号"}]},"item_1722929371688":{"attribute_name":"出版社版DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_language":"ja","subitem_relation_name_text":"10.1186/s12885-019-5715-0"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1186/s12885-019-5715-0","subitem_relation_type_select":"DOI"}}]},"item_1723180141928":{"attribute_name":"EID","attribute_value_mlt":[{"subitem_identifier_type":"URI","subitem_identifier_uri":"356645"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"西條, 早希","creatorNameLang":"ja"},{"creatorName":"サイジョウ, サキ","creatorNameLang":"ja-Kana"},{"creatorName":"Saijo, Saki","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"桑野, 由紀","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"392","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"丹下, 正一朗","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"874","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"六反, 一仁","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"1581","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"西田, 憲生","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"546","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2019-06-20"}],"displaytype":"detail","filename":"k3263_abstract_review.pdf","filesize":[{"value":"227 KB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"objectType":"abstract","url":"https://tokushima-u.repo.nii.ac.jp/record/2006500/files/k3263_abstract_review.pdf"},"version_id":"e14c4b2f-8c4e-42d7-8d19-a9c6ff7b3f12"},{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2019-07-30"}],"displaytype":"detail","filename":"k3263_fulltext.pdf","filesize":[{"value":"1.96 MB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://tokushima-u.repo.nii.ac.jp/record/2006500/files/k3263_fulltext.pdf"},"version_id":"fe5c41a7-d985-4cbd-9c8c-e69c6e172941"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"HOXA5","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"non-coding RNA","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"cell proliferation","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"colon cancer","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"epidermal growth factor signaling","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"A novel long non-coding RNA from the HOXA6-HOXA5 locus facilitates colon cancer cell growth","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"A novel long non-coding RNA from the HOXA6-HOXA5 locus facilitates colon cancer cell growth","subitem_title_language":"en"}]},"item_type_id":"40001","owner":"7","path":["1716268551628"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2019-06-20"},"publish_date":"2019-06-20","publish_status":"0","recid":"2006500","relation_version_is_last":true,"title":["A novel long non-coding RNA from the HOXA6-HOXA5 locus facilitates colon cancer cell growth"],"weko_creator_id":"7","weko_shared_id":-1},"updated":"2024-10-29T00:37:08.679957+00:00"}