{"created":"2024-10-29T00:49:25.598343+00:00","id":2006662,"links":{},"metadata":{"_buckets":{"deposit":"61bf9424-925f-4cdb-8f54-0b9519c6bafe"},"_deposit":{"created_by":7,"id":"2006662","owners":[7],"pid":{"revision_id":0,"type":"depid","value":"2006662"},"status":"published"},"_oai":{"id":"oai:tokushima-u.repo.nii.ac.jp:02006662","sets":["1713853213384:1713853296295:1716267876683:1716268551628"]},"author_link":["162","746","271"],"item_10001_alternative_title_1":{"attribute_name":"タイトル別表記","attribute_value_mlt":[{"subitem_alternative_title":"マウス脳および末梢組織における年齢および性別依存的D-アミノ酸酸化酵素活性 : 加齢および神経変性への影響について","subitem_alternative_title_language":"ja"},{"subitem_alternative_title":"DAO activity exhibits age- and gender-dependence","subitem_alternative_title_language":"en"}]},"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2019-04-02","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"196","bibliographicPageStart":"187","bibliographicVolumeNumber":"166","bibliographic_titles":[{"bibliographic_title":"The Journal of Biochemistry","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"D-amino acid oxidase (DAO) is a flavoenzyme, catalyzing oxidative deamination of D-amino acids to produce corresponding α-keto acids, ammonia and hydrogen peroxide. In our search for DAO activity among various tissues, we developed a sensitive assay based on hydrogen peroxide production involving enzyme-coupled colorimetric assay with peroxidase. We first optimized buffer components to extract DAO protein from mouse tissues. Here we show that DAO activity was detected in kidney, cerebellum, medulla oblongata, midbrain and spinal cord, but not in liver. In addition, we observed that DAO activity and expression were decreased in thoracic and lumbar regions of spinal cord in aged mice when compared to young mice, indicating that decreased DAO is involved in motoneuron degeneration during senescence. We also found gender difference in DAO activity in the kidney, suggesting that DAO activity is influenced by sexual dimorphism. We newly detected DAO activity in the epididymis, although undetected in testis. Furthermore, DAO activity was significantly higher in the caput region than corpus and cauda regions of epididymis, indicating that D-amino acids present in the testis are eliminated in epididymis. Taken together, age- and gender-dependent DAO activity in each organ may underlie the human pathophysiology regulated by D-amino acid metabolism.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"The Japanese Biochemical Society","subitem_publisher_language":"en"}]},"item_10001_source_id_9":{"attribute_name":"収録物ID","attribute_value_mlt":[{"subitem_source_identifier":"0021924X","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"17562651","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"AA12096002","subitem_source_identifier_type":"NCID"},{"subitem_source_identifier":"AA00694073","subitem_source_identifier_type":"NCID"}]},"item_10001_version_type_20":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_be7fb7dd8ff6fe43","subitem_version_type":"NA"}]},"item_1714461018643":{"attribute_name":"報告番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第3316号"}]},"item_1714461102074":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（医学）","subitem_degreename_language":"ja"}]},"item_1714461118377":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2019-05-23"}]},"item_1714461137393":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_language":"ja","subitem_degreegrantor_name":"徳島大学"}]}]},"item_1715043197608":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access"}]},"item_1718868208704":{"attribute_name":"備考","attribute_value_mlt":[{"subitem_textarea_language":"ja","subitem_textarea_value":"内容要旨・審査要旨・論文要約の公開\n本論文は，著者Soo Hyeon Kimの学位論文として提出され，学位審査・授与の対象となっている。\n学位授与者所属 : 徳島大学大学院医科学教育部（医学専攻）"}]},"item_1718868303842":{"attribute_name":"学位記番号","attribute_value_mlt":[{"subitem_text_language":"ja","subitem_text_value":"甲医第1420号"}]},"item_1722929371688":{"attribute_name":"出版社版DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_language":"ja","subitem_relation_name_text":"10.1093/jb/mvz025"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1093/jb/mvz025","subitem_relation_type_select":"DOI"}}]},"item_1723180141928":{"attribute_name":"EID","attribute_value_mlt":[{"subitem_identifier_type":"URI","subitem_identifier_uri":"350159"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"キム, ス ヒョン","creatorNameLang":"ja"},{"creatorName":"キム, ス ヒョン","creatorNameLang":"ja-Kana"},{"creatorName":"Kim, Soo Hyeon","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"宍戸, 裕二","creatorNameLang":"ja"},{"creatorName":"シシド, ユウジ","creatorNameLang":"ja-Kana"},{"creatorName":"Shishido, Yuji","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"ソガベ, ヒロフミ","creatorNameLang":"ja"},{"creatorName":"ソガベ, ヒロフミ","creatorNameLang":"ja-Kana"},{"creatorName":"Sogabe, Hirofumi","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Rachadech, Wanitcha","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"頼田, 和子","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"162","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"加藤, 有介","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"746","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"福井, 清","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"271","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2019-08-19"}],"displaytype":"detail","filename":"k3316_abstract.pdf","filesize":[{"value":"101 KB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"objectType":"abstract","url":"https://tokushima-u.repo.nii.ac.jp/record/2006662/files/k3316_abstract.pdf"},"version_id":"5614a2c1-c7ca-4390-a45e-200a6c36f823"},{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2019-08-19"}],"displaytype":"detail","filename":"k3316_review.pdf","filesize":[{"value":"84.6 KB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"objectType":"other","url":"https://tokushima-u.repo.nii.ac.jp/record/2006662/files/k3316_review.pdf"},"version_id":"ae7ec1b0-1cb1-4e8a-817d-9197fed0273c"},{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2019-08-19"}],"displaytype":"detail","filename":"k3316_summary.pdf","filesize":[{"value":"117 KB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"objectType":"summary","url":"https://tokushima-u.repo.nii.ac.jp/record/2006662/files/k3316_summary.pdf"},"version_id":"e43f9a79-f62f-49f9-82f7-d80209c9989f"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"age- and gender-dependence","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"D-amino acid oxidase","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"epididymis","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"kidney","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"spinal cord","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Age- and gender-dependent D-amino acid oxidase activity in mouse brain and peripheral tissues : implication for aging and neurodegeneration","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Age- and gender-dependent D-amino acid oxidase activity in mouse brain and peripheral tissues : implication for aging and neurodegeneration","subitem_title_language":"en"}]},"item_type_id":"40001","owner":"7","path":["1716268551628"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2019-08-19"},"publish_date":"2019-08-19","publish_status":"0","recid":"2006662","relation_version_is_last":true,"title":["Age- and gender-dependent D-amino acid oxidase activity in mouse brain and peripheral tissues : implication for aging and neurodegeneration"],"weko_creator_id":"7","weko_shared_id":-1},"updated":"2024-10-29T00:49:37.870612+00:00"}