{"created":"2024-10-29T01:08:13.120477+00:00","id":2006904,"links":{},"metadata":{"_buckets":{"deposit":"837e6fcd-9c29-43df-ad91-1080ae7f4849"},"_deposit":{"created_by":7,"id":"2006904","owners":[7],"pid":{"revision_id":0,"type":"depid","value":"2006904"},"status":"published"},"_oai":{"id":"oai:tokushima-u.repo.nii.ac.jp:02006904","sets":["1713853213384:1713853295607"]},"author_link":["1242","1032"],"item_10001_alternative_title_1":{"attribute_name":"タイトル別表記","attribute_value_mlt":[{"subitem_alternative_title":"Binding affinity of poly-γ-glutamate to Shiga toxin","subitem_alternative_title_language":"en"}]},"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2018-03-04","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"5","bibliographicPageStart":"e12538","bibliographicVolumeNumber":"42","bibliographic_titles":[{"bibliographic_title":"Journal of Food Biochemistry","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"We examined poly-γ-glutamate from natto, a Japanese fermented food, in the ability to adsorb Shiga toxin 1 (Stx1) and Shiga toxin 2 (Stx2). The polymer was immobilized by direct coupling to EAH-SepharoseTM. The poly-γ-glutamate-Sepharose (about 10 mg of ligand/mL of gel) adsorbed Stx2, but not Stx1: its dissociation constant (Kd) against Stx2 was calculated to be 14.0 μM. To analyze the binding site of poly-γ-glutamate against Stx2, we similarly immobilized glutamate and glutarate. Glutamate- and glutarate-Sepharoses (each 7 μmol of ligand/mL of gel) similarly adsorbed Stx2, but not Stx1; Kd values against Stx2 were calculated to be 14.0 and 30.0, respectively, μM. The common structures of PGA-, glutamate-, and glutarate-Sepharoses were considered to be glutaryl groups. When we added the mixture of Stx2 and poly-γ-glutamate-Sepharose to Caco-2 cells (a human colon epithelial cell line), poly-γ-glutamate-Sepharose was found to reduce the cytotoxicity of Stx2.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Wiley Periodicals","subitem_publisher_language":"en"}]},"item_10001_source_id_9":{"attribute_name":"収録物ID","attribute_value_mlt":[{"subitem_source_identifier":"17454514","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_1715043197608":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access"}]},"item_1718868208704":{"attribute_name":"備考","attribute_value_mlt":[{"subitem_textarea_language":"ja","subitem_textarea_value":"This is the peer reviewed version of the following article: Kanemaru, K, Goto, T, Badr, HA, Yokoigawa, K. Determination of binding affinity of poly‐γ‐glutamate to Shiga toxin. J Food Biochem. 2018; 42:e12538, which has been published in final form at https://doi.org/10.1111/jfbc.12538. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions."}]},"item_1722929371688":{"attribute_name":"出版社版DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_language":"ja","subitem_relation_name_text":"10.1111/jfbc.12538"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1111/jfbc.12538","subitem_relation_type_select":"DOI"}}]},"item_1723180141928":{"attribute_name":"EID","attribute_value_mlt":[{"subitem_identifier_type":"URI","subitem_identifier_uri":"336716"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"金丸, 芳","creatorNameLang":"ja"},{"creatorName":"カネマル, カヲリ","creatorNameLang":"ja-Kana"},{"creatorName":"Kanemaru, Kaori","creatorNameLang":"en"}],"familyNames":[{"familyName":"金丸","familyNameLang":"ja"},{"familyName":"カネマル","familyNameLang":"ja-Kana"},{"familyName":"Kanemaru","familyNameLang":"en"}],"givenNames":[{"givenName":"芳","givenNameLang":"ja"},{"givenName":"カヲリ","givenNameLang":"ja-Kana"},{"givenName":"Kaori","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1032","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"00243676","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://nrid.nii.ac.jp/ja/search/?qm=00243676"}]},{"creatorNames":[{"creatorName":"ゴトウ, ツキエ","creatorNameLang":"ja"},{"creatorName":"ゴトウ, ツキエ","creatorNameLang":"ja-Kana"},{"creatorName":"Goto, Tsukie","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Badr, Hoida Ali","creatorNameLang":"en"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"横井川, 久己男","creatorNameLang":"ja"},{"creatorName":"ヨコイガワ, クミオ","creatorNameLang":"ja-Kana"},{"creatorName":"Yokoigawa, Kumio","creatorNameLang":"en"}],"familyNames":[{"familyName":"横井川","familyNameLang":"ja"},{"familyName":"ヨコイガワ","familyNameLang":"ja-Kana"},{"familyName":"Yokoigawa","familyNameLang":"en"}],"givenNames":[{"givenName":"久己男","givenNameLang":"ja"},{"givenName":"クミオ","givenNameLang":"ja-Kana"},{"givenName":"Kumio","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1242","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"82339/profile-ja.html","nameIdentifierScheme":"徳島大学 教育研究者総覧","nameIdentifierURI":"http://pub2.db.tokushima-u.ac.jp/ERD/person/82339/profile-ja.html"},{"nameIdentifier":"60230637","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://nrid.nii.ac.jp/ja/search/?qm=60230637"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2019-11-08"}],"displaytype":"detail","filename":"jfbc_42_5_e12538.pdf","filesize":[{"value":"1.95 MB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://tokushima-u.repo.nii.ac.jp/record/2006904/files/jfbc_42_5_e12538.pdf"},"version_id":"4da3df77-6fb1-41d8-9336-54f3e6de7dcb"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Escherichia coli O157:H7","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"poly-γ-glutamate","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Shiga toxin","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Determination of binding affinity of poly‐γ‐glutamate to Shiga toxin","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Determination of binding affinity of poly‐γ‐glutamate to Shiga toxin","subitem_title_language":"en"}]},"item_type_id":"40001","owner":"7","path":["1713853295607"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2019-11-08"},"publish_date":"2019-11-08","publish_status":"0","recid":"2006904","relation_version_is_last":true,"title":["Determination of binding affinity of poly‐γ‐glutamate to Shiga toxin"],"weko_creator_id":"7","weko_shared_id":-1},"updated":"2025-02-10T07:50:05.463177+00:00"}