| Item type |
文献 / Documents(1) |
| 公開日 |
2020-03-13 |
| アクセス権 |
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アクセス権 |
open access |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 出版社版DOI |
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関連識別子 |
https://doi.org/10.1371/journal.pbio.3000262 |
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関連名称 |
10.1371/journal.pbio.3000262 |
| 出版タイプ |
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出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
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|
タイトル |
Regulation of membrane phospholipid asymmetry by Notch-mediated flippase expression controls the number of intraepithelial TCRαβ+CD8αα+ T cells |
| タイトル別表記 |
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その他のタイトル |
Notch controls the number of intraepithelial TCRαβ+CD8αα+ T cells |
| 著者 |
石舟, 智恵子
九十九, 伸一
マエカワ, ヨウイチ
ホズミ, カツト
Chung, Doo Hyun
モトゾノ, チヒロ
ヤマサキ, ショウ
ナカノ, ヒロヤス
安友, 康二
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| 抄録 |
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内容記述 |
Intestinal intraepithelial lymphocytes (IELs) expressing CD8αα on αβ T cells (TCRαβ+CD8αα+ IELs) have suppressive capabilities in enterocolitis, but the mechanism that maintains homeostasis and cell number is not fully understood. Here, we demonstrated that the number of TCRαβ+CD8αα+ IELs was severely reduced in mice lacking recombination signal binding protein for immunoglobulin kappa J region (Rbpj) or Notch1 and Notch2 in T cells. Rbpj-deficient TCRαβ+CD8αα+ IELs expressed low levels of Atp8a2, which encodes a protein with flippase activity that regulates phospholipid asymmetry of plasma membrane such as flipping phosphatidylserine in the inner leaflet of plasma membrane. Rbpj-deficient TCRαβ+CD8αα+ IELs cannot maintain phosphatidylserine in the inner leaflet of the plasma membrane. Furthermore, depletion of intestinal macrophages restored TCRαβ+CD8αα+ IELs in Rbpj-deficient mice, suggesting that exposure of phosphatidylserine on the plasma membrane in Rbpj-deficient TCRαβ+CD8αα+ IELs acts as an “eat-me” signal. Together, these results revealed that Notch–Atp8a2 is a fundamental regulator for IELs and highlighted that membrane phospholipid asymmetry controlled by Notch-mediated flippase expression is a critical determinant in setting or balancing the number of TCRαβ+CD8αα+ IELs. |
| 書誌情報 |
en : PLOS Biology
巻 17,
号 5,
p. e3000262,
発行日 2019-05-09
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| 収録物ID |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
15449173 |
| 収録物ID |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
15457885 |
| 出版者 |
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出版者 |
PLOS |
| 権利情報 |
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|
権利情報 |
© 2019 Ishifune et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
| EID |
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|
識別子 |
351891 |
| 言語 |
|
|
言語 |
eng |
pbio_17_5_e3000262.pdf (3.34 MB)
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