| Item type |
文献 / Documents(1) |
| 公開日 |
2020-04-23 |
| アクセス権 |
|
|
アクセス権 |
open access |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版社版DOI |
|
|
|
関連識別子 |
https://doi.org/10.1042/BCJ20190716 |
|
|
関連名称 |
10.1042/BCJ20190716 |
| 出版タイプ |
|
|
出版タイプ |
AM |
|
出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
| タイトル |
|
|
タイトル |
All-trans retinoic acid reduces the transcriptional regulation of intestinal sodium-dependent phosphate co-transporter gene (Npt2b) |
| タイトル別表記 |
|
|
その他のタイトル |
Regulation of Npt2b gene promoter activity by RAR/RXR-C/EBP |
| 著者 |
増田, 真志
山本, 浩範
タケイ, ユウイチロウ
ナカハシ, オトキ
アダチ, ユウイチロウ
大西, 康太
大南, 博和
(山中)奥村, 仙示
阪上, 浩
ミヤザキ, マコト
武田, 英二
竹谷, 豊
|
| 抄録 |
|
|
内容記述 |
Inorganic phosphate (Pi) homeostasis is regulated by intestinal absorption via type II sodium-dependent co-transporter (Npt2b) and by renal reabsorption via Npt2a and Npt2c. Although we previously reported that vitamin A-deficient (VAD) rats had increased urine Pi excretion through the decreased renal expression of Npt2a and Npt2c, the effect of vitamin A on the intestinal Npt2b expression remains unclear. In this study, we investigated the effects of treatment with all-trans retinoic acid (ATRA), a metabolite of vitamin A, on the Pi absorption and the Npt2b expression in the intestine of VAD rats, as well as and the underlying molecular mechanisms. In VAD rats, the intestinal Pi uptake activity and the expression of Npt2b were increased, but were reduced by the administration of ATRA. The transcriptional activity of reporter plasmid containing the promoter region of the rat Npt2b gene was reduced by ATRA in NIH3T3 cells overexpressing retinoic acid receptor (RAR) and retinoid X receptor (RXR). On the other hand, CCAAT/enhancer-binding proteins (C/EBP) induced transcriptional activity of the Npt2b gene. Knockdown of the C/EBP gene and a mutation analysis of the C/EBP responsible element in the Npt2b gene promoter indicated that C/EBP plays a pivotal role in the regulation of Npt2b gene transcriptional activity by ATRA. EMSA revealed that the RAR/RXR complex inhibits binding of C/EBP to Npt2 b gene promoter. Together, these results suggest that ATRA may reduce the intestinal Pi uptake by preventing C/EBP activation of the intestinal Npt2b gene. |
| キーワード |
|
|
主題 |
small intestine |
| キーワード |
|
|
主題 |
all-trans retinoic acid (ATRA) |
| キーワード |
|
|
主題 |
transporter |
| キーワード |
|
|
主題 |
gene promoter analysis |
| キーワード |
|
|
主題 |
CCAAT-enhancer-binding protein (C/EBP) |
| キーワード |
|
|
主題 |
phosphate homeostasis |
| キーワード |
|
|
主題 |
retinoic acid receptor (RAR) |
| キーワード |
|
|
主題 |
type IIb sodium-dependent phosphate co-transporter (Npt2b) |
| 書誌情報 |
en : Biochemical Journal
巻 477,
号 4,
p. 817-831,
発行日 2020-02-28
|
| 収録物ID |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
02646021 |
| 収録物ID |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
14708728 |
| 収録物ID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA10649144 |
| 収録物ID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA12366617 |
| 収録物ID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA12072285 |
| 出版者 |
|
|
出版者 |
Portland Press |
| 出版者 |
|
|
出版者 |
The Biochemical Society |
| 備考 |
|
|
値 |
This is not the final published Version of Record.
The final authenticated version is available online at: https://doi.org/10.1042/BCJ20190716. |
| EID |
|
|
識別子 |
363852 |
| 言語 |
|
|
言語 |
eng |
bcj_477_4_817.pdf (2.54 MB)
